The DOI matches its Crossref record; 78 of the 78 references it cites were found in a registry or an index.
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses
Open accessAbstract
There is need for effective and affordable vaccines against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of coronavirus spike glycoprotein receptor-binding domain (RBD) on a synthetic virus-like particle (VLP) platform, SpyCatcher003-mi3, using SpyTag/SpyCatcher technology. Low doses of RBD-SpyVLP in a prime-boost regimen induce a strong neutralising antibody response in mice and pigs that is superior to convalescent human sera. We evaluate antibody quality using ACE2 blocking and neutralisation of cell infection by pseudovirus or wild-type SARS-CoV-2. Using competition assays with a monoclonal antibody panel, we show that RBD-SpyVLP induces a polyclonal antibody response that recognises key epitopes on the RBD, reducing the likelihood of selecting neutralisation-escape mutants. Moreover, RBD-SpyVLP is thermostable and can be lyophilised without losing immunogenicity, to facilitate global distribution and reduce cold-chain dependence. The data suggests that RBD-SpyVLP provides strong potential to address clinical and logistic challenges of the COVID-19 pandemic.
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Cited by
Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models
Cohen, A. A., van Doremalen, N., Greaney, A. J., Andersen, H., Sharma, A., Starr, T. N., Keeffe, J. R., Fan, C., Schulz, J. E., Gnanapragasam, P. N. P., et al. (2022) · Science · doi:10.1126/science.abq0839 · Cited by 259
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Metadata
- Authors
- Tan, T. K., Rijal, P., Rahikainen, R., Keeble, A. H., Schimanski, L., Hussain, S., Harvey, R., Hayes, J. W. P., Edwards, J. C., McLean, R. K., Martini, V., Pedrera, M., Thakur, N., Conceicao, C., Dietrich, I., Shelton, H., Ludi, A., Wilsden, G., Browning, C., Zagrajek, A. K., Bialy, D., Bhat, S., Stevenson-Leggett, P., Hollinghurst, P., Tully, M., Moffat, K., Chiu, C., Waters, R., Gray, A., Azhar, M., Mioulet, V., Newman, J., Asfor, A. S., Burman, A., Crossley, S., Hammond, J. A., Tchilian, E., Charleston, B., Bailey, D., Tuthill, T. J., Graham, S. P., Duyvesteyn, H. M. E., Malinauskas, T., Huo, J., Tree, J. A., Buttigieg, K. R., Owens, R. J., Carroll, M. W., Daniels, R. S., McCauley, J. W., Stuart, D. I., Huang, K.-Y. A., Howarth, M., & Townsend, A. R.
- Venue
- Nature Communications
- Published
- 2021-01-22
- Volume
- 12
- Issue
- 1
- Pages
- 542-542
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3126017645
- Cited by
- 283
- References
- 78
- FWCI
- 16.88
- Type
- journal article
- Language
- en
- Open access
- gold
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- Tan, T. K., Rijal, P., Rahikainen, R., Keeble, A. H., Schimanski, L., Hussain, S., Harvey, R., Hayes, J. W. P., Edwards, J. C., McLean, R. K., Martini, V., Pedrera, M., Thakur, N., Conceicao, C., Dietrich, I., Shelton, H., Ludi, A., Wilsden, G., Browning, C., . . . Townsend, A. R. (2021). A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses. Nature Communications. https://doi.org/10.1038/s41467-020-20654-7
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
