The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis
Open accessAbstract
Phosphorus is an essential nutrient taken up by organisms in the form of inorganic phosphate (Pi). Eukaryotes have evolved sophisticated Pi sensing and signaling cascades, enabling them to stably maintain cellular Pi concentrations. Pi homeostasis is regulated by inositol pyrophosphate signaling molecules (PP-InsPs), which are sensed by SPX domain-containing proteins. In plants, PP-InsP-bound SPX receptors inactivate Myb coiled-coil (MYB-CC) Pi starvation response transcription factors (PHRs) by an unknown mechanism. Here we report that a InsP 8 –SPX complex targets the plant-unique CC domain of PHRs. Crystal structures of the CC domain reveal an unusual four-stranded anti-parallel arrangement. Interface mutations in the CC domain yield monomeric PHR1, which is no longer able to bind DNA with high affinity. Mutation of conserved basic residues located at the surface of the CC domain disrupt interaction with the SPX receptor in vitro and in planta, resulting in constitutive Pi starvation responses. Together, our findings suggest that InsP 8 regulates plant Pi homeostasis by controlling the oligomeric state and hence the promoter binding capability of PHRs via their SPX receptors.
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“In Arabidopsis , inositol pyrophosphate (InsP 8 ) concentration increases under phosphate-rich conditions and promotes the interaction between SPX domains and a four-stranded coiled-coil motif of phosphate starvation response transcription factors 36 .”
BackgroundRecent Progress of Molecular Mechanisms of DNA Methylation in Plant Response to Abiotic Stress
Lu, X., Liu, Y., Xu, J., Liu, X., Chi, Y., Li, R., Mo, L., Shi, L., Liang, S., Yu, W., et al. (2024) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2023.105599 · Cited by 22
Identification of vacuolar phosphate influx transporters in Brassica napus
Han, B., Wang, C., Wu, T., Yan, J., Jiang, A., Liu, Y., Luo, Y., Cai, H., Ding, G., Dong, X., et al. (2022) · Plant, Cell & Environment · doi:10.1111/pce.14423 · Cited by 21
A small signaling domain controls PPIP5K phosphatase activity in phosphate homeostasis
Raia, P., Lee, K., Bartsch, S. M., Rico-Resendiz, F., Portugal-Calisto, D., Vadas, O., Panse, V. G., Fiedler, D., & Hothorn, M. (2025) · Nature Communications · doi:10.1038/s41467-025-56937-0 · Cited by 9
Self-citationThe potent PHL4 transcription factor effector domain contains significant disorder
Fonda, B. D., & Murray, D. T. (2024) · Protein Science · doi:10.1002/pro.5214 · Cited by 1
A small signaling domain controls PPIP5K phosphatase activity in phosphate homeostasis
Raia, P., Lee, K., Bartsch, S. M., Rico-Resendiz, F., Portugal-Calisto, D., Vadas, O., Panse, V. G., Fiedler, D., & Hothorn, M. (2024) · doi:10.1101/2024.09.12.612650
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Metadata
- Authors
- Ried, M. K., Wild, R., Zhu, J., Pipercevic, J., Sturm, K., Broger, L., Harmel, R. K., Abriata, L. A., Hothorn, L. A., Fiedler, D., Hiller, S., & Hothorn, M.
- Venue
- Nature Communications
- Published
- 2021-01-15
- Volume
- 12
- Issue
- 1
- Pages
- 384-384
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3122999147
- Cited by
- 233
- References
- 6
- FWCI
- 19.28
- Type
- journal article
- Language
- en
- Open access
- gold
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