Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins
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Abstract
Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation was independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis.
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Specialized Ribosomes in Health and Disease
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A Ribosomal Perspective on Neuronal Local Protein Synthesis
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eIF3 mRNA selectivity profiling reveals eIF3k as a cancer‐relevant regulator of ribosome content
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Logistics of neuronal protein turnover: Numbers and mechanisms
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The Beak of Eukaryotic Ribosomes: Life, Work and Miracles
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SPIN-AI: A Deep Learning Model That Identifies Spatially Predictive Genes
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Dynamic SILAC to Determine Protein Turnover in Neurons and Glia
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Self-citationTranslational Remodeling of the Synaptic Proteome During Aging
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A deep dive into functional ribosome specialization
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- Authors
- Fusco, C. M., Desch, K., Dörrbaum, A. R., Wang, M., Staab, A., Chan, I. C. W., Vail, E., Villeri, V., Langer, J. D., & Schuman, E. M.
- Affiliations
- Max Planck Institute for Brain Research (DE)
- German Center for Neurodegenerative Diseases (DE)
- Université de Bordeaux (FR)
- Max Planck Institute of Biophysics (DE)
- Corresponding author
- Schuman, Erin M.
- ORCID
- Venue
- Nature Communications
- Published
- 2021-10-21
- Volume
- 12
- Issue
- 1
- Pages
- 6127-6127
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC8531293
- OpenAlex
- W3207078712
- Cited by
- 157
- References
- 84
- Citations by year
- 2026: 23 · 2025: 43 · 2024: 38 · 2023: 27 · 2022: 23 · 2021: 3
- FWCI
- 7.38
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DZNE Pub (submittedVersion, other-oa)
- Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) (submittedVersion, cc-by)
- Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) (submittedVersion, cc-by-nc-nd)
- Funders
- Deutsche Forschungsgemeinschaft (CRC 1080, DFG-CRC902, CRC902)
- Max-Planck-Gesellschaft (DFG CRC 1080)
- H2020 European Research Council (743216)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- ribosomal proteins, local protein synthesis, dendrites, axons, neuropil, ribosome biogenesis, oxidative stress, translation machinery, ribosome profiling, metabolic labeling
- MeSH terms
- Animals, Axons, Cells, Cultured, Female, Male, Neurons, Ribosomal Proteins, Ribosomes, RNA, Messenger, Protein Biosynthesis, Neurites, Rats, Sprague-Dawley, Neuropil, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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