Cited by 161RNA and protein synthesis mechanismsRNA Research and SplicingNeuroscience and Neuropharmacology Research

Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins

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Fusco, Claudia M., Desch, Kristina, Dörrbaum, Aline R., Wang, Mantian, Staab, Anja, Chan, Ivy C. W., Vail, Eleanor, Villeri, Veronica +2 more·2021·Nature Communications

Published version of a preprint

Abstract

Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation was independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis.

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Authors
Fusco, C. M., Desch, K., Dörrbaum, A. R., Wang, M., Staab, A., Chan, I. C. W., Vail, E., Villeri, V., Langer, J. D., & Schuman, E. M.
Affiliations
  • Max Planck Institute for Brain Research (DE)
  • German Center for Neurodegenerative Diseases (DE)
  • Université de Bordeaux (FR)
  • Max Planck Institute of Biophysics (DE)
Corresponding author
Schuman, Erin M.
Venue
Nature Communications
Published
2021-10-21
Volume
12
Issue
1
Pages
6127-6127
ISSN
2041-1723 (ISSN-L)
OpenAlex
W3207078712
Cited by
157
References
84
Citations by year
2026: 23 · 2025: 43 · 2024: 38 · 2023: 27 · 2022: 23 · 2021: 3
FWCI
7.38
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DZNE Pub (submittedVersion, other-oa)
  • Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) (submittedVersion, cc-by)
  • Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) (submittedVersion, cc-by-nc-nd)
Funders
  • Deutsche Forschungsgemeinschaft (CRC 1080, DFG-CRC902, CRC902)
  • Max-Planck-Gesellschaft (DFG CRC 1080)
  • H2020 European Research Council (743216)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
ribosomal proteins, local protein synthesis, dendrites, axons, neuropil, ribosome biogenesis, oxidative stress, translation machinery, ribosome profiling, metabolic labeling
MeSH terms
Animals, Axons, Cells, Cultured, Female, Male, Neurons, Ribosomal Proteins, Ribosomes, RNA, Messenger, Protein Biosynthesis, Neurites, Rats, Sprague-Dawley, Neuropil, Rats

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