CoChat
Sign Up
Cited by 38Trypanosoma species research and implicationsResearch on Leishmaniasis StudiesGenetic and Kidney Cyst Diseases

A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission

Open access
Bachmaier, Sabine, Giacomelli, Giacomo, Calvo-Alvarez, Estefanía, Vieira, Larissa Rezende, Van Den Abbeele, Jan, Aristodemou, Aris, Lorentzen, Esben, Gould, Matt K. +8 more·2022·Nature Communications
DOI 10.1038/s41467-022-33108-zPMC PMC9481589

Published version of a preprint

Open this paper in CoChat — free accountOpen PDF

Abstract

Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonization and SoMo. Cyclic AMP response protein 3 (CARP3) binds and regulates multiple AC isoforms. CARP3 tip localization depends on the cytoskeletal protein FLAM8. Re-localization of CARP3 away from the tip microdomain is sufficient to abolish SoMo and fly SG colonization. Since intrinsic development is normal in carp3 and flam8 knock-out parasites, AC complex-mediated tip signaling specifically controls parasite migration and thereby transmission. Participation of several developmentally regulated receptor-type AC isoforms may indicate the complexity of the in vivo signals perceived.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41467-022-33108-z

Cites

7 of 7 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Authors
Bachmaier, S., Giacomelli, G., Calvo-Alvarez, E., Vieira, L. R., Van Den Abbeele, J., Aristodemou, A., Lorentzen, E., Gould, M. K., Brennand, A., Dupuy, J.-W., Forné, I., Imhof, A., Bramkamp, M., Salmon, D., Rotureau, B., & Boshart, M.
Affiliations
  • Ludwig-Maximilians-Universität München (DE)
  • Christian-Albrechts-Universität zu Kiel (DE)
  • Universidade Federal do Rio de Janeiro (BR)
  • Aarhus University (DK)
  • Université de Bordeaux (FR)
  • Inserm (FR)
Corresponding author
  • Bachmaier, Sabine
  • Boshart, Michael
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2022-09-16
Volume
13
Issue
1
Pages
5445-5445
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-022-33108-z
PMC
PMC9481589
OpenAlex
W4296041492
Cited by
38
References
7
Citations by year
2026: 3 · 2025: 7 · 2024: 10 · 2023: 17 · 2022: 1
FWCI
6.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Open access LMU (Ludwid Maxmilian's Universitat Munchen) (acceptedVersion)
  • HAL (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ludwig-Maximilians-Universität München
  • BioNa young scientists award
  • IN.WBI excellence fellowship
  • Deutsche Forschungsgemeinschaft (443931024)
  • Institut Pasteur
Topics
  • Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
African trypanosomes, Trypanosoma brucei, adenylate cyclase, cAMP signaling, social motility, parasite migration, trypanosome transmission, ciliary signaling
MeSH terms
Cyclic AMP, Adenylyl Cyclases, Animals, Trypanosoma, Trypanosoma brucei brucei, Tsetse Flies

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review