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Cited by 11HIV Research and TreatmentT-cell and Retrovirus StudiesEnzyme Structure and Function

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The crystal structure of a simian Foamy Virus receptor binding domain provides clues about entry into host cells

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Fernández, Ignacio, Dynesen, Lasse Toftdal, Coquin, Youna, Pederzoli, Riccardo, Brun, Delphine, Haouz, Ahmed, Gessain, Antoine, Rey, Félix A., Buseyne, Florence, Backovic, Marija·2023·Nature Communications
DOI 10.1038/s41467-023-36923-0PMC PMC9988990

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Abstract

The surface envelope glycoprotein (Env) of all retroviruses mediates virus binding to cells and fusion of the viral and cellular membranes. A structure-function relationship for the HIV Env that belongs to the Orthoretrovirus subfamily has been well established. Structural information is however largely missing for the Env of Foamy viruses (FVs), the second retroviral subfamily. In this work we present the X-ray structure of the receptor binding domain (RBD) of a simian FV Env at 2.57 Å resolution, revealing two subdomains and an unprecedented fold. We have generated a model for the organization of the RBDs within the trimeric Env, which indicates that the upper subdomains form a cage-like structure at the apex of the Env, and identified residues K342, R343, R359 and R369 in the lower subdomain as key players for the interaction of the RBD and viral particles with heparan sulfate.

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Metadata

Authors
Fernández, I., Dynesen, L. T., Coquin, Y., Pederzoli, R., Brun, D., Haouz, A., Gessain, A., Rey, F. A., Buseyne, F., & Backovic, M.
Affiliations
Centre National de la Recherche Scientifique (FR)
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2023-03-06
Volume
14
Issue
1
Pages
1262-1262
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-023-36923-0
PMC
PMC9988990
OpenAlex
W4323310853
Cited by
11
References
1
Citations by year
2026: 4 · 2025: 2 · 2024: 3 · 2023: 2
FWCI
1.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Agence Nationale de la Recherche (ANR‐10‐LABX‐62‐IBEID, ANR-10, ANR-10-LABX-0062)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Keywords
simian foamy virus, receptor binding domain, envelope glycoprotein, viral entry, host cell receptors, heparan sulfate, cage-like structure, X-ray crystal structure
MeSH terms
Cell Membrane, Membrane Glycoproteins, Retroviridae, Spumavirus, Simian foamy virus

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