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Cited by 91Fungal and yeast genetics researchFungal Infections and StudiesStudies on Chitinases and Chitosanases

Structural adaptation of fungal cell wall in hypersaline environment

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Fernando, Liyanage D., Pérez-Llano, Yordanis, Dickwella Widanage, Malitha C., Jacob, Anand, Martínez-Ávila, Liliana, Lipton, Andrew S., Gunde-Cimerman, Nina, Latgé, Jean-Paul +2 more·2023·Nature Communications
DOI 10.1038/s41467-023-42693-6PMC PMC10625518

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Abstract

Halophilic fungi thrive in hypersaline habitats and face a range of extreme conditions. These fungal species have gained considerable attention due to their potential applications in harsh industrial processes, such as bioremediation and fermentation under unfavorable conditions of hypersalinity, low water activity, and extreme pH. However, the role of the cell wall in surviving these environmental conditions remains unclear. Here we employ solid-state NMR spectroscopy to compare the cell wall architecture of Aspergillus sydowii across salinity gradients. Analyses of intact cells reveal that A. sydowii cell walls contain a rigid core comprising chitin, β-glucan, and chitosan, shielded by a surface shell composed of galactomannan and galactosaminogalactan. When exposed to hypersaline conditions, A. sydowii enhances chitin biosynthesis and incorporates α-glucan to create thick, stiff, and hydrophobic cell walls. Such structural rearrangements enable the fungus to adapt to both hypersaline and salt-deprived conditions, providing a robust mechanism for withstanding external stress. These molecular principles can aid in the optimization of halophilic strains for biotechnology applications.

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  • Reduced water permeability is a common response previously observed in several Aspergillus species under stress.

    “Both C. albicans and C. auris AR386 exhibit reduced water permeability (Fig. 4a), a common response previously also observed in several Aspergillus species under stress50,53.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [53]

    The Introduction and Discussion report limited water permeability in A. sydowii, including under high-salt conditions. The Results section on other halophilic Aspergillus species reports shared cell-wall composition in A. atacamensis and A. destruens, but does not establish reduced water permeability in those species. The claim that this response was observed in several Aspergillus species is therefore broader than this source supports. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • The balance between rigid structural components and mobile, water-accessible regions allows fungi to fine-tune cell wall permeability, aiding adaptive survival.

    “Recent observations suggest that the balance between rigid structural components and mobile, water-accessible regions allows fungi to fine-tune cell wall permeability, aiding adaptive survival50,65.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [65]

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Authors
Fernando, L. D., Pérez-Llano, Y., Dickwella Widanage, M. C., Jacob, A., Martínez-Ávila, L., Lipton, A. S., Gunde-Cimerman, N., Latgé, J.-P., Batista-García, R. A., & Wang, T.
Affiliations
  • University of Georgia (US)
  • Michigan State University (US)
  • Universidad Autónoma del Estado de Morelos (MX)
  • Pacific Northwest National Laboratory (US)
  • University of Crete (GR)
Corresponding author
Wang, Tuo
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2023-11-04
Volume
14
Issue
1
Pages
7082-7082
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-023-42693-6
PMC
PMC10625518
OpenAlex
W4388348390
Cited by
91
References
4
Citations by year
2026: 26 · 2025: 47 · 2024: 17 · 2023: 1
FWCI
8.11
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • U.S. Department of Health & Human Services | National Institutes of Health (AI173270)
  • U.S. Department of Energy (DE-AC05-76RL01830)
  • Javna Agencija za Raziskovalno Dejavnost RS (P4-0432)
  • Consejo Nacional de Ciencia y Tecnología (CB-285816)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Studies on Chitinases and Chitosanases
Keywords
halophilic fungi, fungal cell wall, hypersalinity, Aspergillus sydowii, chitin synthesis, β-glucan, chitosan, galactomannan, galactosaminogalactan, α-glucan, salt stress adaptation, salt deprivation, low water activity, bioremediation
MeSH terms
Cell Wall, Chitin, Glucans, Sodium Chloride, beta-Glucans

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