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Cited by 72Bacteriophages and microbial interactionsBacterial Genetics and BiotechnologyEvolution and Genetic Dynamics

Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency

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Haudiquet, Matthieu, Le Bris, Julie, Nucci, Amandine, Bonnin, Rémy A., Domingo-Calap, Pilar, Rocha, Eduardo P. C., Rendueles, Olaya·2024·Nature Communications
DOI 10.1038/s41467-024-46147-5PMC PMC10918111
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Abstract

Bacterial evolution is affected by mobile genetic elements like phages and conjugative plasmids, offering new adaptive traits while incurring fitness costs. Their infection is affected by the bacterial capsule. Yet, its importance has been difficult to quantify because of the high diversity of confounding mechanisms in bacterial genomes such as anti-viral systems and surface receptor modifications. Swapping capsule loci between Klebsiella pneumoniae strains allowed us to quantify their impact on plasmid and phage infection independently of genetic background. Capsule swaps systematically invert phage susceptibility, revealing serotypes as key determinants of phage infection. Capsule types also influence conjugation efficiency in both donor and recipient cells, a mechanism shaped by capsule volume and conjugative pilus structure. Comparative genomics confirmed that more permissive serotypes in the lab correspond to the strains acquiring more conjugative plasmids in nature. The least capsule-sensitive pili (F-like) are the most frequent in the species’ plasmids, and are the only ones associated with both antibiotic resistance and virulence factors, driving the convergence between virulence and antibiotics resistance in the population. These results show how traits of cellular envelopes define slow and fast lanes of infection by mobile genetic elements, with implications for population dynamics and horizontal gene transfer.

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Cited by

Sorted by citations

Authors
Haudiquet, M., Le Bris, J., Nucci, A., Bonnin, R. A., Domingo-Calap, P., Rocha, E. P. C., & Rendueles, O.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Inserm (FR)
Corresponding author
  • Haudiquet, Matthieu
  • Rocha, Eduardo P. C.
  • Rendueles, Olaya
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-03-06
Volume
15
Issue
1
Pages
2032-2032
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-024-46147-5
PMC
PMC10918111
OpenAlex
W4392508205
Cited by
72
References
2
Citations by year
2026: 36 · 2025: 30 · 2024: 5 · 2023: 1
FWCI
19.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Infrastructures en Biologie Santé et Agronomie
  • Agence Nationale de la Recherche (10-LABX-62-IBEID, ANR-16-CE15-0022, ANR- 10-INBS-09, ANR 18 CE12 0001 01, ANR‐10‐LABX‐62‐IBEID, ANR 16 CE15 0022 03 PREDIRES, ANR 18 CE12 0001 01 ENCAPSULATION, ANR-16-CONV-0005, 18 CE12 0001 01, ANR-18-CE12-0001, ANR-10, ANR-10-LABX-0062, 10-INBS-09, ANR-10-LABX-62)
  • Fondation pour la Recherche Médicale (EQU201903007835)
  • Agencia Estatal de Investigación (AEI//10.13039/501100011033/, 501100011033, by MCIN/AEI/10.13039/501100011033, 10.13039/501100011033, MCIN/AEI/10.13039/501 100 011 033, 13039, .13039/501100011033, 10.13039, AEI/10)
  • European Social Fund (MCIN/AEI/10.13039/ 501100011033, 501100011033, AEI/10.13039/501100011033)
Topics
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
bacterial capsule, phage susceptibility, conjugative plasmids, conjugation efficiency, Klebsiella pneumoniae, serotypes, F pilus, horizontal gene transfer, mobile genetic elements
MeSH terms
Bacteriophages, Phenotype, Plasmids, Genome, Bacterial, Serogroup

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