The rumen microbiome: an underexplored resource for novel antimicrobial discovery
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Abstract
Abstract Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.
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The rumen remains a source of valuable bioactives for the biotechnology industry, and metagenomics is a key tool for such bioprospecting.
“The rumen also remains a source of valuable bioactives for the biotechnology industry, and metagenomics is a key tool for such bioprospecting (Oyama et al., 2017; Roumpeka et al., 2017).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Oyama et al., 2017
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- Authors
- Oyama, L. B., Girdwood, S. E., Cookson, A. R., Fernandez-Fuentes, N., Privé, F., Vallin, H. E., Wilkinson, T. J., Golyshin, P. N., Golyshina, O. V., Mikut, R., Hilpert, K., Richards, J., Wootton, M., Edwards, J. E., Maresca, M., Perrier, J., Lundy, F. T., Luo, Y., Zhou, M., Hess, M., Mantovani, H. C., Creevey, C. J., & Huws, S. A.
- Affiliations
- Aberystwyth University (GB)
- Bangor University (GB)
- Karlsruhe Institute of Technology (DE)
- St George's, University of London (GB)
- University Hospital of Wales (GB)
- Wageningen University & Research (NL)
- Centre National de la Recherche Scientifique (FR)
- Queen's University Belfast (GB)
- University of California, Davis (US)
- Corresponding author
- Huws, Sharon A.
- ORCID
- Venue
- npj Biofilms and Microbiomes
- Published
- 2017-12-01
- Volume
- 3
- Issue
- 1
- Pages
- 33-33
- ISSN
- 2055-5008 (ISSN-L)
- PMC
- PMC5711939
- OpenAlex
- W2734956387
- Cited by
- 80
- References
- 46
- Citations by year
- 2026: 8 · 2025: 12 · 2024: 9 · 2023: 7 · 2022: 9 · 2021: 9 · 2020: 8 · 2019: 14 · 2018: 4
- FWCI
- 5.76
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- npj Biofilms and Microbiomes (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- Repository KITopen (Karlsruhe Institute of Technology) (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Americanae (AECID Library) (publishedVersion, other-oa)
- Research Portal (Queen's University Belfast) (publishedVersion, cc-by)
- St George's Online Research Archive (St George's University of London) (acceptedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
- Americanae (AECID Library) (submittedVersion, other-oa)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
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- Bangor University Research Portal (Bangor University) (submittedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
- KITopen (cc-by)
- Funders
- Research Councils UK (BB/L026716/1, 172629373)
- Life Sciences Research Network Wales
- Biotechnology and Biological Sciences Research Council (M029085, 172629373, BB/M029085/1, BBS/E/W/0012843D, BB/M029085, BB/L026716/1, ERA-IB-14-030, BBS/E/W/10964A01D, BB/L026716/2)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- rumen microbiome, antimicrobial peptides, antimicrobial drug discovery, metagenome mining, methicillin-resistant Staphylococcus aureus, bacterial membrane permeability, bacterial membrane lipids, topical antimicrobial therapy, wound infection, multidrug-resistant bacteria
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