CoChat
Sign Up
Cited by 98

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Fam49/CYRI interacts with Rac1 and locally suppresses protrusions

Fort, Loic, Batista, José Miguel, Thomason, Peter A., Spence, Heather J., Whitelaw, Jamie A., Tweedy, Luke, Greaves, Jennifer, Martin, Kirsty J., Anderson, Kurt I., Brown, Peter, Lilla, Sergio, Neilson, Matthew P., Tafelmeyer, Petra, Zanivan, Sara, Ismail, Shehab, Bryant, David M., Tomkinson, Nicholas C. O., Chamberlain, Luke H., Mastick, Grant S., Insall, Robert H., Machesky, Laura M.·2018·Nature Cell Biology
DOI 10.1038/s41556-018-0198-9PMC PMC6863750

Published version of a preprint

Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41556-018-0198-9

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 4 references

Research works printed but not linked: 67

  1. Krause, M. & Gautreau, A. Steering cell migration: lamellipodium dynamics and the regulation of directional persistence. Nat. Rev. Mol. Cell Biol. 15, 577–590 (2014). · DOI
  2. Insall, R. The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration. Curr. Opin. Cell Biol. 25, 526–531 (2013). · DOI
  3. Davidson, A. J. & Insall, R. H. Actin-based motility: WAVE regulatory complex structure reopens old SCARs. Curr. Biol. 21, R66–R68 (2011). · DOI
  4. Hoeller, O. et al. Gβ regulates coupling between actin oscillators for cell polarity and directional migration. PLoS Biol. 14, e1002381 (2016). · DOI
  5. Veltman, D. M., King, J. S., Machesky, L. M. & Insall, R. H. SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. J. Cell Biol. 198, 501–508 (2012). · DOI
  6. Weiner, O. D. et al. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. PLoS Biol. 4, e38 (2006). · DOI
  7. Andrew, N. & Insall, R. H. Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions. Nat. Cell Biol. 9, 193–200 (2007). · DOI
  8. Devreotes, P. N. et al. Excitable signal transduction networks in directed cell migration. Annu. Rev. Cell Dev. Biol. 33, 103–125 (2017). · DOI
  9. Graziano, B. R. & Weiner, O. D. Self-organization of protrusions and polarity during eukaryotic chemotaxis. Curr. Opin. Cell Biol. 30, 60–67 (2014). · DOI
  10. Maritzen, T. et al. Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex. Proc. Natl Acad. Sci. USA 109, 10382–10387 (2012). · DOI
  11. Dang, I. et al. Inhibitory signalling to the Arp2/3 complex steers cell migration. Nature 503, 281–284 (2013). · DOI · Paper page
  12. Sobczyk, G. J., Wang, J. & Weijer, C. J. SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale. Nat. Commun. 5, 3319 (2014). · DOI
  13. Veltman, D. M. & Insall, R. H. WASP family proteins: their evolution and its physiological implications. Mol. Biol. Cell 21, 2880–2893 (2010). · DOI
  14. Bramham, C. R., Jensen, K. B. & Proud, C. G. Tuning specific translation in cancer metastasis and synaptic memory: control at the MNK–eIF4E axis. Trends Biochem. Sci. 41, 847–858 (2016). · DOI
  15. Bienvenut, W. V. et al. Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features. Mol. Cell. Proteomics 11, M111.015131 (2012). · DOI
  16. Broncel, M. et al. Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development. Angew. Chem. Int. Ed. Engl. 54, 5948–5951 (2015). · DOI
  17. Yang, W., Di Vizio, D., Kirchner, M., Steen, H. & Freeman, M. R. Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol. Cell. Proteomics 9, 54–70 (2010). · DOI
  18. Lanyon-Hogg, T., Faronato, M., Serwa, R. A. & Tate, E. W. Dynamic protein acylation: new substrates, mechanisms, and drug targets. Trends Biochem. Sci. 42, 566–581 (2017). · DOI
  19. Chen, B. et al. Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites. eLife 6, e29795 (2017). · DOI · Paper page
  20. Soderberg, O. et al. Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay. Methods 45, 227–232 (2008). · DOI
  21. Robinson, M. S., Sahlender, D. A. & Foster, S. D. Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria. Dev. Cell 18, 324–331 (2010). · DOI
  22. Walmsley, M. J. et al. Critical roles for Rac1 and Rac2 GTPases in B cell development and signaling. Science 302, 459–462 (2003). · DOI · Paper page
  23. Li, A. et al. Activated mutant NRas(Q61K) drives aberrant melanocyte signaling, survival, and invasiveness via a Rac1-dependent mechanism. J. Invest. Dermatol. 132, 2610–2621 (2012). · DOI
  24. Li, A. et al. Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod-driven motility and cell-cycle progression. Dev. Cell 21, 722–734 (2011). · DOI
  25. Steffen, A. et al. Rac function is crucial for cell migration but is not required for spreading and focal adhesion formation. J. Cell Sci. 126, 4572–4588 (2013).
  26. Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D. & Hall, A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70, 401–410 (1992). · DOI
  27. Martin, K. J. et al. Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments. PLoS ONE 13, e0183585 (2018). · DOI
  28. Nakamura, T., Kurokawa, K., Kiyokawa, E. & Matsuda, M. Analysis of the spatiotemporal activation of rho GTPases using Raichu probes. Methods Enzymol. 406, 315–332 (2006). · DOI
  29. Wu, Y. I. et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461, 104–108 (2009). · DOI
  30. Keren, K., Yam, P. T., Kinkhabwala, A., Mogilner, A. & Theriot, J. A. Intracellular fluid flow in rapidly moving cells. Nat. Cell Biol. 11, 1219–1224 (2009). · DOI
  31. Pankov, R. et al. A Rac switch regulates random versus directionally persistent cell migration. J. Cell Biol. 170, 793–802 (2005). · DOI
  32. Insall, R. & Andrew, N. Chemotaxis in Dictyostelium: how to walk straight using parallel pathways. Curr. Opin. Microbiol. 10, 578–581 (2007). · DOI
  33. Muinonen-Martin, A. J. et al. Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biol. 12, e1001966 (2014). · DOI
  34. Tweedy, L., Knecht, D. A., Mackay, G. M. & Insall, R. H. Self-generated chemoattractant gradients: attractant depletion extends the range and robustness of chemotaxis. PLoS Biol. 14, e1002404 (2016). · DOI
  35. Kuspa, A. & Loomis, W. F. Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc. Natl Acad. Sci. USA 89, 8803–8807 (1992). · DOI
  36. Meinhardt, H. Orientation of chemotactic cells and growth cones: models and mechanisms. J. Cell Sci. 112, 2867–2874 (1999). · DOI
  37. Neilson, M. P., Mackenzie, J. A., Webb, S. D. & Insall, R. H. Use of the parameterised finite element method to robustly and efficiently evolve the edge of a moving cell. Integr. Biol. 2, 687–695 (2010). · DOI
  38. Mack, N. A. et al. β2-syntrophin and Par-3 promote an apicobasal Rac activity gradient at cell–cell junctions by differentially regulating Tiam1 activity. Nat. Cell Biol. 14, 1169–1180 (2012). · DOI
  39. Mangan, A. J. et al. Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells. Nat. Commun. 7, 12426 (2016). · DOI
  40. Overeem, A. W., Bryant, D. M. & van Ijzendoorn, S. C. D. Mechanisms of apical–basal axis orientation and epithelial lumen positioning. Trends Cell Biol. 25, 476–485 (2015). · DOI
  41. Yagi, S., Matsuda, M. & Kiyokawa, E. Suppression of Rac1 activity at the apical membrane of MDCK cells is essential for cyst structure maintenance. EMBO Rep. 13, 237–243 (2012). · DOI
  42. Jiang, H. et al. Protein lipidation: occurrence, mechanisms, biological functions, and enabling technologies. Chem. Rev. 118, 919–988 (2018). · DOI
  43. Grobe, H., Wustenhagen, A., Baarlink, C., Grosse, R. & Grikscheit, K. A Rac1–FMNL2 signaling module affects cell–cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE 13, e0194716 (2018). · DOI
  44. Madasu, Y. et al. PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner. Mol. Biol. Cell 26, 1308–1322 (2015). · DOI
  45. Meacci, G. et al. ⍺-Actinin links extracellular matrix rigidity-sensing contractile units with periodic cell-edge retractions. Mol. Biol. Cell 27, 3471–3479 (2016). · DOI
  46. Swaminathan, K., Muller-Taubenberger, A., Faix, J., Rivero, F. & Noegel, A. A. A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of Coronin. Proc. Natl Acad. Sci. USA 111, E25–E33 (2014). · DOI
  47. Swaminathan, K. et al. Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins. Sci. Rep. 5, 14437 (2015). · DOI
  48. Gerisch, G. et al. Mobile actin clusters and traveling waves in cells recovering from actin depolymerization. Biophys. J. 87, 3493–3503 (2004). · DOI
  49. Killich, T. et al. The locomotion, shape and pseudopodial dynamics of unstimulated Dictyostelium cells are not random. J. Cell Sci. 106, 1005–1013 (1993). · DOI
  50. Tweedy, L., Susanto, O. & Insall, R. H. Self-generated chemotactic gradients-cells steering themselves. Curr. Opin. Cell Biol. 42, 46–51 (2016). · DOI
  51. Kollmar, M., Lbik, D. & Enge, S. Evolution of the eukaryotic ARP2/3 activators of the WASP family: WASP, WAVE, WASH, and WHAMM, and the proposed new family members WAWH and WAML. BMC Res. Notes 5, 88 (2012). · DOI
  52. Wheeler, T. J., Clements, J. & Finn, R. D. Skylign: a tool for creating informative, interactive logos representing sequence alignments and profile hidden Markov models. BMC Bioinformatics 15, 7 (2014). · DOI
  53. Kelley, L. A. & Sternberg, M. J. Protein structure prediction on the Web: a case study using the Phyre server. Nat. Protoc. 4, 363–371 (2009). · DOI
  54. Greaves, J. et al. Molecular basis of fatty acid selectivity in the zDHHC family of S-acyltransferases revealed by click chemistry. Proc. Natl Acad. Sci. USA 114, E1365–E1374 (2017). · DOI
  55. Vojtek, A. B. & Hollenberg, S. M. Ras–Raf interaction: two-hybrid analysis. Methods Enzymol. 255, 331–342 (1995). · DOI
  56. Bartel, P., Chien, C. T., Sternglanz, R. & Fields, S. Elimination of false positives that arise in using the two-hybrid system. Biotechniques 14, 920–924 (1993).
  57. Fromont-Racine, M., Rain, J. C. & Legrain, P. Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nat. Genet. 16, 277–282 (1997). · DOI
  58. Ran, F. A. et al. Genome engineering using the CRISPR–Cas9 system. Nat. Protoc. 8, 2281–2308 (2013). · DOI · Paper page
  59. Martin, K. J. et al. Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments. PLoS ONE 13, e0183585 (2018). · DOI
  60. Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012). · DOI · Paper page
  61. Ibarra, N., Blagg, S. L., Vazquez, F. & Insall, R. H. Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-dependent and -independent pathways. Curr. Biol. 16, 717–722 (2006). · DOI
  62. McGarry, D. J., Shchepinova, M. M., Lilla, S., Hartley, R. C. & Olson, M. F. A cell-permeable biscyclooctyne as a novel probe for the identification of protein sulfenic acids. ACS Chem. Biol. 11, 3300–3304 (2016). · DOI
  63. Cox, J. & Mann, M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat. Biotechnol. 26, 1367–1372 (2008). · DOI · Paper page
  64. Fey, P., Dodson, R. J., Basu, S. & Chisholm, R. L. One stop shop for everything Dictyostelium: dictyBase and the Dicty Stock Center in 2012. Methods Mol. Biol. 983, 59–92 (2013). · DOI
  65. Keller, A., Nesvizhskii, A. I., Kolker, E. & Aebersold, R. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal. Chem. 74, 5383–5392 (2002). · DOI
  66. Veltman, D. M., Akar, G., Bosgraaf, L. & Van Haastert, P. J. A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid 61, 110–118 (2009). · DOI
  67. Woznica, D. & Knecht, D. A. Under-agarose chemotaxis of Dictyostelium discoideum. Methods Mol. Biol. 346, 311–325 (2006).

Cited by

See all 98

Metadata

Authors
Fort, L., Batista, J. M., Thomason, P. A., Spence, H. J., Whitelaw, J. A., Tweedy, L., Greaves, J., Martin, K. J., Anderson, K. I., Brown, P., Lilla, S., Neilson, M. P., Tafelmeyer, P., Zanivan, S., Ismail, S., Bryant, D. M., Tomkinson, N. C. O., Chamberlain, L. H., Mastick, G. S., Insall, R. H., & Machesky, L. M.
ORCID
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2018-09-24
Volume
20
Issue
10
Pages
1159-1171
ISSN
  • 1465-7392
  • 1476-4679
DOI
10.1038/s41556-018-0198-9
PMC
PMC6863750
Cited by
98
References
4
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. https://app.cochat.ai/papers/10.1038/s41556-018-0198-9
The paper
Fort, L., Batista, J. M., Thomason, P. A., Spence, H. J., Whitelaw, J. A., Tweedy, L., Greaves, J., Martin, K. J., Anderson, K. I., Brown, P., Lilla, S., Neilson, M. P., Tafelmeyer, P., Zanivan, S., Ismail, S., Bryant, D. M., Tomkinson, N. C. O., Chamberlain, L. H., Mastick, G. S., . . . Machesky, L. M. (2018). Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nature Cell Biology. https://doi.org/10.1038/s41556-018-0198-9