The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Dynamic biofilm architecture confers individual and collective mechanisms of viral protection
Open accessAbstract
In nature, bacteria primarily live in surface-attached, multicellular communities, termed biofilms1–6. In medical settings, biofilms cause devastating damage during chronic and acute infections; indeed, bacteria are often viewed as agents of human disease7. However, bacteria themselves suffer from diseases, most notably in the form of viral pathogens termed bacteriophages8–12, which are the most abundant replicating entities on Earth. Phage–biofilm encounters are undoubtedly common in the environment, but the mechanisms that determine the outcome of these encounters are unknown. Using Escherichia coli biofilms and the lytic phage T7 as models, we discovered that an amyloid fibre network of CsgA (curli polymer) protects biofilms against phage attack via two separate mechanisms. First, collective cell protection results from inhibition of phage transport into the biofilm, which we demonstrate in vivo and in vitro. Second, CsgA fibres protect cells individually by coating their surface and binding phage particles, thereby preventing their attachment to the cell exterior. These insights into biofilm–phage interactions have broad-ranging implications for the design of phage applications in biotechnology, phage therapy and the evolutionary dynamics of phages with their bacterial hosts. At late stages of biofilm development, Escherichia coli cells express the curli polymer CsgA. CsgA assembles into a fibre network that protects biofilms from attack by lytic phages.
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Emergence of three-dimensional order and structure in growing biofilms
Hartmann, R., Singh, P. K., Pearce, P., Mok, R., Song, B., Díaz-Pascual, F., Dunkel, J., & Drescher, K. (2019) · Nature Physics · doi:10.1038/s41567-018-0356-9 · Cited by 283
Self-citationWang, F., Cvirkaite-Krupovic, V., Krupovic, M., & Egelman, E. H. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2207037119 · Cited by 30
The Probiotic Escherichia coli Strain Nissle 1917 Combats Lambdoid Bacteriophages stx and λ
Bury, S., Soundararajan, M., Bharti, R., von Bünau, R., Förstner, K. U., & Oelschlaeger, T. A. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00929 · Cited by 21
Metadata
- Authors
- Vidakovic, L., Singh, P. K., Hartmann, R., Nadell, C. D., & Drescher, K.
- Venue
- Nature Microbiology
- Published
- 2017-10-30
- Volume
- 3
- Issue
- 1
- Pages
- 26-31
- ISSN
- 2058-5276 (ISSN-L)
- OpenAlex
- W2766272099
- Cited by
- 265
- References
- 43
- FWCI
- 16.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Vidakovic, L., Singh, P. K., Hartmann, R., Nadell, C. D., & Drescher, K. (2017). Dynamic biofilm architecture confers individual and collective mechanisms of viral protection. Nature Microbiology. https://doi.org/10.1038/s41564-017-0050-1
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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