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Cited by 21Escherichia coli research studiesBacteriophages and microbial interactionsBacterial Genetics and Biotechnology

The Probiotic Escherichia coli Strain Nissle 1917 Combats Lambdoid Bacteriophages stx and λ

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Bury, Susanne, Soundararajan, Manonmani, Bharti, Richa, von Bünau, Rudolf, Förstner, Konrad U., Oelschlaeger, Tobias A.·2018·Frontiers in Microbiology
DOI 10.3389/fmicb.2018.00929PMC PMC5987069
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Abstract

Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coli strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype O104:H4, responsible for the great outbreak in 2011 in Germany, evolutionary developed from certain E. coli strains which got infected by stx2-encoding lambdoid phages turning the E. coli into lysogenic and subsequently Stx producing strains. Since antibiotics induce stx genes and Stx production, EHEC infected persons are not recommended to be treated with antibiotics. Therefore, EcN might be an alternative medication. However, because even commensal E. coli strains might be converted into Stx-producers after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN towards not only stx-phages but also against lambda-phages. This resistance was not based on the lack of or by mutated phage receptors. Rather it involved the expression of a phage repressor (pr) gene of a defective prophage in EcN which was able to partially protect E. coli K-12 strain MG1655 against stx and lambda phage infection. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.

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  • Preclinical studies have demonstrated efficacy of phages targeting colibactin-producing Escherichia coli.

    “and colibactin-producing Escherichia coli [66]”

    From Phage therapy in oncology: opportunities for cancer prevention and treatment (2026) · cites this paper as [66]

    The Results section ('EcN protects K-12 strains from phage infection'; page unavailable) reports that probiotic E. coli reduced stx-phage levels, not that phages targeted colibactin-producing E. coli. A search for colibactin and preclinical efficacy found no passage in our copy addressing that claim. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Bury, S., Soundararajan, M., Bharti, R., von Bünau, R., Förstner, K. U., & Oelschlaeger, T. A.
Affiliations
University of Würzburg (DE)
Corresponding author
Oelschlaeger, Tobias A.
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2018-05-29
Volume
9
Pages
929-929
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2018.00929
PMC
PMC5987069
OpenAlex
W2807130658
Cited by
21
References
6
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 1
FWCI
1.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Online Publication Service of Würzburg University (Würzburg University) (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Escherichia coli Nissle 1917, probiotics, Shiga toxin-producing Escherichia coli, enterohemorrhagic Escherichia coli, bacteriophage lambda, prophage integration, phage resistance, phage inactivation, thermostable proteins, foodborne illness prevention

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