The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Emergence of three-dimensional order and structure in growing biofilms
Open accessAbstract
Surface-attached bacterial biofilms are self-replicating active liquid crystals and the dominant form of bacterial life on Earth1–4. In conventional liquid crystals and solid-state materials, the interaction potentials between the molecules that comprise the system determine the material properties. However, for growth-active biofilms it is unclear whether potential-based descriptions can account for the experimentally observed morphologies, and which potentials would be relevant. Here, we have overcome previous limitations of single-cell imaging techniques5,6 to reconstruct and track all individual cells inside growing three-dimensional biofilms with up to 10,000 individuals. Based on these data, we identify, constrain and provide a microscopic basis for an effective cell–cell interaction potential, which captures and predicts the growth dynamics, emergent architecture and local liquid-crystalline order of Vibrio cholerae biofilms. Furthermore, we show how external fluid flows control the microscopic structure and three-dimensional morphology of biofilms. Our analysis implies that local cellular order and global biofilm architecture in these active bacterial communities can arise from mechanical cell–cell interactions, which cells can modulate by regulating the production of particular matrix components. These results establish an experimentally validated foundation for improved continuum theories of active matter and thereby contribute to solving the important problem of controlling biofilm growth. Single-cell tracking of up to 10,000 bacteria reveals the structure and dynamics of 3D biofilms—providing evidence to suggest that both local ordering and global biofilm architecture emerge from mechanical interactions.
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Cites
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The Mechanical World of Bacteria
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Self-citationPositive selection vectors for allelic exchange
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Living in the matrix: assembly and control of Vibrio cholerae biofilms
Teschler, J. K., Zamorano-Sánchez, D., Utada, A. S., Warner, C. J. A., Wong, G. C. L., Linington, R. G., & Yildiz, F. H. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3433 · Cited by 412
Molecular Architecture and Assembly Principles of Vibrio cholerae Biofilms
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Role of Multicellular Aggregates in Biofilm Formation
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Cited by
Quantitative image analysis of microbial communities with BiofilmQ
Hartmann, R., Jeckel, H., Jelli, E., Singh, P. K., Vaidya, S., Bayer, M., Rode, D. K. H., Vidakovic, L., Díaz-Pascual, F., Fong, J. C. N., et al. (2021) · Nature Microbiology · doi:10.1038/s41564-020-00817-4 · Cited by 365
Self-citationMechanomicrobiology: how bacteria sense and respond to forces
Dufrêne, Y. F., & Persat, A. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0314-2 · Cited by 299
Odd dynamics of living chiral crystals
Tan, T. H., Mietke, A., Li, J., Chen, Y., Higinbotham, H., Foster, P. J., Gokhale, S., Dunkel, J., & Fakhri, N. (2022) · Nature · doi:10.1038/s41586-022-04889-6 · Cited by 280
Self-citationBiofilms deform soft surfaces and disrupt epithelia
Cont, A., Rossy, T., Al-Mayyah, Z., & Persat, A. (2020) · eLife · doi:10.7554/elife.56533 · Cited by 70
Jelli, E., Ohmura, T., Netter, N., Abt, M., Jiménez‐Siebert, E., Neuhaus, K., Rode, D. K. H., Nadell, C. D., & Drescher, K. (2023) · Molecular Microbiology · doi:10.1111/mmi.15064 · Cited by 48
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Metadata
- Authors
- Hartmann, R., Singh, P. K., Pearce, P., Mok, R., Song, B., Díaz-Pascual, F., Dunkel, J., & Drescher, K.
- Venue
- Nature Physics
- Published
- 2018-11-26
- Volume
- 15
- Issue
- 3
- Pages
- 251-256
- ISSN
- 1745-2473 (ISSN-L)
- 1745-2481
- OpenAlex
- W2900643491
- Cited by
- 283
- References
- 35
- FWCI
- 8.48
- Type
- journal article
- Language
- en
- Open access
- bronze
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