The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Role of nanoscale antigen organization on B-cell activation probed using DNA origami
Open accessAbstract
Vaccine efficacy can be increased by arraying immunogens in multivalent form on virus-like nanoparticles to enhance B-cell activation. However, the effects of antigen copy number, spacing and affinity, as well as the dimensionality and rigidity of scaffold presentation on B-cell activation remain poorly understood. Here, we display the clinical vaccine immunogen eOD-GT8, an engineered outer domain of the HIV-1 glycoprotein-120, on DNA origami nanoparticles to systematically interrogate the impact of these nanoscale parameters on B-cell activation in vitro. We find that B-cell signalling is maximized by as few as five antigens maximally spaced on the surface of a 40-nm viral-like nanoparticle. Increasing antigen spacing up to ~25–30 nm monotonically increases B-cell receptor activation. Moreover, scaffold rigidity is essential for robust B-cell triggering. These results reveal molecular vaccine design principles that may be used to drive functional B-cell responses. DNA origami allows the precise spatial patterning of antigens to investigate the impact of antigen spacing and arrangement on B-cell activation in vitro, which is important for the design of efficient vaccination strategies.
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Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
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Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012) · Nature Methods · doi:10.1038/nmeth.2089 · Cited by 64,638
Folding DNA to create nanoscale shapes and patterns
Rothemund, P. W. K. (2006) · Nature · doi:10.1038/nature04586 · Cited by 7,354
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Bachmann, M. F., & Jennings, G. T. (2010) · Nature Reviews Immunology · doi:10.1038/nri2868 · Cited by 2,106
Differential activation of transcription factors induced by Ca2+ response amplitude and duration
Dolmetsch, R. E., Lewis, R. S., Goodnow, C. C., & Healy, J. I. (1997) · Nature · doi:10.1038/386855a0 · Cited by 1,752
Distribution and three-dimensional structure of AIDS virus envelope spikes
Zhu, P., Liu, J., Bess, J., Chertova, E., Lifson, J. D., Grisé, H., Ofek, G. A., Taylor, K. A., & Roux, K. H. (2006) · Nature · doi:10.1038/nature04817 · Cited by 780
Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors
Jardine, J., Julien, J.-P., Menis, S., Ota, T., Kalyuzhniy, O., McGuire, A., Sok, D., Huang, P.-S., MacPherson, S., Jones, M., et al. (2013) · Science · doi:10.1126/science.1234150 · Cited by 770
Self-citationThe Influence of Antigen Organization on B Cell Responsiveness
Bachmann, M. F., Rohrer, U. H., Kündig, T. M., Bürki, K., Hengartner, H., & Zinkernagel, R. M. (1993) · Science · doi:10.1126/science.8248784 · Cited by 717
B cells acquire antigen from target cells after synapse formation
Batista, F. D., Iber, D., & Neuberger, M. S. (2001) · Nature · doi:10.1038/35078099 · Cited by 626
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Veneziano, R., Ratanalert, S., Zhang, K., Zhang, F., Yan, H., Chiu, W., & Bathe, M. (2016) · Science · doi:10.1126/science.aaf4388 · Cited by 620
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Metadata
- Authors
- Veneziano, R., Moyer, T. J., Stone, M. B., Wamhoff, E.-C., Read, B. J., Mukherjee, S., Shepherd, T. R., Das, J., Schief, W. R., Irvine, D. J., & Bathe, M.
- Venue
- Nature Nanotechnology
- Published
- 2020-06-29
- Volume
- 15
- Issue
- 8
- Pages
- 716-723
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W3037346400
- Cited by
- 429
- References
- 56
- FWCI
- 17.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Veneziano, R., Moyer, T. J., Stone, M. B., Wamhoff, E.-C., Read, B. J., Mukherjee, S., Shepherd, T. R., Das, J., Schief, W. R., Irvine, D. J., & Bathe, M. (2020). Role of nanoscale antigen organization on B-cell activation probed using DNA origami. Nature Nanotechnology. https://doi.org/10.1038/s41565-020-0719-0
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