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Engineering organoids
Open accessAbstract
Organoids are in vitro miniaturized and simplified model systems of organs that have gained enormous interest for modelling tissue development and disease, and for personalized medicine, drug screening and cell therapy. Despite considerable success in culturing physiologically relevant organoids, challenges remain to achieve real-life applications. In particular, the high variability of self-organizing growth and restricted experimental and analytical access hamper the translatability of organoid systems. In this Review, we argue that many limitations of traditional organoid culture can be addressed by engineering approaches at all levels of organoid systems. We investigate cell surface and genetic engineering approaches, and discuss stem cell niche engineering based on the design of matrices that allow spatiotemporal control of organoid growth and shape-guided morphogenesis. We examine how microfluidic approaches and lessons learnt from organs-on-a-chip enable the integration of mechano-physiological parameters and increase accessibility of organoids to improve functional readouts. Applying engineering principles to organoids increases reproducibility and provides experimental control, which will, ultimately, be required to enable clinical translation.
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“Although Matrigel provides important biochemical cues, it has an ill-defined chemistry and composition, and major lot-to-lot variation [18,19], and thus offers a poor control over ECM composition [20].”
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Gong, X., Wen, Z., Liang, Z., Xiao, H., Lee, S., Wright, T., Nguyen, R. Y., Rossello‐Martinez, A., & Mak, M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.08.561456 · Cited by 2
Hofer, M., Correa, M. A. D., & Lütolf, M. P. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.26.577381 · Cited by 2
Self-citationIlluminating morphogen and patterning dynamics with optogenetic control of morphogen productionPreprint
Benzinger, D., & Briscoe, J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.11.598403 · Cited by 2
3D Microphysiological Tumor Model for Dual-Targeting CAR T Cell ImmunotherapyPreprint
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Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar DisorderPreprint
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Hyaluronan Underlies the Emergence of Form, Fate, and Function in Human CardioidsPreprint
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All-in-one approach to culture, image and quantify large numbers of organoids at all scales
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Metadata
- Authors
- Hofer, M., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Venue
- Nature Reviews Materials
- Published
- 2021-02-19
- Volume
- 6
- Issue
- 5
- Pages
- 402-420
- ISSN
- 2058-8437 (ISSN-L)
- PMC
- PMC7893133
- OpenAlex
- W4211240480
- Cited by
- 1,184
- References
- 277
- Citations by year
- 2026: 189 · 2025: 275 · 2024: 256 · 2023: 226 · 2022: 168 · 2021: 70
- FWCI
- 15.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Reviews Materials (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- National Science Foundation
- European Commission (H2020-MSCA-ITN, H2020-MSCA-ITN-2018, HORIZON2020, H2020, 668294, MSCA-ITN-2018, H2020-MSCA)
- École Polytechnique Fédérale de Lausanne
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (179447, 310030)
- Vienna Science and Technology Fund
- Board of the Swiss Federal Institutes of Technology
- Horizon 2020 Framework Programme (MSCA‐ITN‐2018, H2020-MSCA, 668,294, H2020-MSCA-ITN-2018, H2020-MSCA-ITN)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- organoids, organoid engineering, organoid culture, cell surface engineering, genetic engineering, morphogenesis, microfluidics, organ-on-a-chip, mechanobiology, reproducibility, translational medicine, drug screening, personalized medicine
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