The DOI matches its Crossref record; 90 of the 90 references it cites were found in a registry or an index.
Fast and sensitive GCaMP calcium indicators for imaging neural populations
Open accessAbstract
Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here we used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators 3–8 . The resulting ‘jGCaMP8’ sensors, based on the calcium-binding protein calmodulin and a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (half-rise times of 2 ms) and the highest sensitivity for neural activity reported for a protein-based calcium sensor. jGCaMP8 sensors will allow tracking of large populations of neurons on timescales relevant to neural computation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Coot : model-building tools for molecular graphics
Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571
Kabsch, W. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909047337 · Cited by 16,463
Refinement of Macromolecular Structures by the Maximum-Likelihood Method
Murshudov, G. N., Vagin, A. A., & Dodson, E. J. (1997) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444996012255 · Cited by 14,742
Ultrasensitive fluorescent proteins for imaging neuronal activity
Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
Self-citationilastik: interactive machine learning for (bio)image analysis
Berg, S., Kutra, D., Kroeger, T., Straehle, C. N., Kausler, B. X., Haubold, C., Schiegg, M., Ales, J., Beier, T., Rudy, M., et al. (2019) · Nature Methods · doi:10.1038/s41592-019-0582-9 · Cited by 3,368
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Miyawaki, A., Llopis, J., Heim, R., McCaffery, J. M., Adams, J. A., Ikura, M., & Tsien, R. Y. (1997) · Nature · doi:10.1038/42264 · Cited by 3,248
Xu, C., & Webb, W. W. (1996) · Journal of the Optical Society of America B · doi:10.1364/josab.13.000481 · Cited by 2,198
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
Tian, L., Hires, S. A., Mao, T., Huber, D., Chiappe, M. E., Chalasani, S. H., Petreanu, L., Akerboom, J., McKinney, S. A., Schreiter, E. R., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1398 · Cited by 1,967
Self-citationA high signal-to-noise Ca2+ probe composed of a single green fluorescent protein
Nakai, J., Ohkura, M., & Imoto, K. (2001) · Nature Biotechnology · doi:10.1038/84397 · Cited by 1,616
Cited by
Functional connectomics spanning multiple areas of mouse visual cortex
The MICrONS Consortium, Bae, J. A., Baptiste, M., Bishop, C. A., Bodor, A. L., Brittain, D., Buchanan, J., Bumbarger, D. J., Castro, M. A., Celii, B., et al. (2021) · doi:10.1101/2021.07.28.454025 · Cited by 214
Directed evolution of adeno-associated virus for efficient gene delivery to microglia
Lin, R., Zhou, Y., Yan, T., Wang, R., Li, H., Wu, Z., Zhang, X., Zhou, X., Zhao, F., Zhang, L., et al. (2022) · Nature Methods · doi:10.1038/s41592-022-01547-7 · Cited by 171
Dong, C., Zheng, Y., Long-Iyer, K., Wright, E. C., Li, Y., & Tian, L. (2022) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-110520-031137 · Cited by 99
Human iPSC 4R tauopathy model uncovers modifiers of tau propagation
Parra Bravo, C., Giani, A. M., Madero-Perez, J., Zhao, Z., Wan, Y., Samelson, A. J., Wong, M. Y., Evangelisti, A., Cordes, E., Fan, L., et al. (2024) · Cell · doi:10.1016/j.cell.2024.03.015 · Cited by 90
Gap junctions desynchronize a neural circuit to stabilize insect flight
Hürkey, S., Niemeyer, N., Schleimer, J.-H., Ryglewski, S., Schreiber, S., & Duch, C. (2023) · Nature · doi:10.1038/s41586-023-06099-0 · Cited by 64
Metadata
- Authors
- Zhang, Y., Rózsa, M., Liang, Y., Bushey, D., Wei, Z., Zheng, J., Reep, D., Broussard, G. J., Tsang, A., Tsegaye, G., Narayan, S., Obara, C. J., Lim, J.-X., Patel, R., Zhang, R., Ahrens, M. B., Turner, G. C., Wang, S. S.-H., Korff, W. L., Schreiter, E. R., Svoboda, K., Hasseman, J. P., Kolb, I., & Looger, L. L.
- Venue
- Nature
- Published
- 2023-03-15
- Volume
- 615
- Issue
- 7954
- Pages
- 884-891
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W4324344199
- Cited by
- 870
- References
- 90
- FWCI
- 93.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Fast and sensitive GCaMP calcium indicators for imaging neural populations. https://app.cochat.ai/papers/10.1038/s41586-023-05828-9
- The paper
- Zhang, Y., Rózsa, M., Liang, Y., Bushey, D., Wei, Z., Zheng, J., Reep, D., Broussard, G. J., Tsang, A., Tsegaye, G., Narayan, S., Obara, C. J., Lim, J.-X., Patel, R., Zhang, R., Ahrens, M. B., Turner, G. C., Wang, S. S.-H., Korff, W. L., . . . Looger, L. L. (2023). Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature. https://doi.org/10.1038/s41586-023-05828-9
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
