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Cited by 99Advanced Fluorescence Microscopy TechniquesNeuroscience and Neuropharmacology ResearchNeural dynamics and brain function

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Fluorescence Imaging of Neural Activity, Neurochemical Dynamics, and Drug-Specific Receptor Conformation with Genetically Encoded Sensors

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Dong, Chunyang, Zheng, Yu, Long-Iyer, Kiran, Wright, Emily C., Li, Yulong, Tian, Lin·2022·Annual Review of Neuroscience
DOI 10.1146/annurev-neuro-110520-031137PMC PMC9940643
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Abstract

Recent advances in fluorescence imaging permit large-scale recording of neural activity and dynamics of neurochemical release with unprecedented resolution in behaving animals. Calcium imaging with highly optimized genetically encoded indicators provides a mesoscopic view of neural activity from genetically defined populations at cellular and subcellular resolutions. Rigorously improved voltage sensors and microscopy allow for robust spike imaging of populational neurons in various brain regions. In addition, recent protein engineering efforts in the past few years have led to the development of sensors for neurotransmitters and neuromodulators. Here, we discuss the development and applications of these genetically encoded fluorescent indicators in reporting neural activity in response to various behaviors in different biological systems as well as in drug discovery. We also report a simple model to guide sensor selection and optimization.

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Metadata

Authors
Dong, C., Zheng, Y., Long-Iyer, K., Wright, E. C., Li, Y., & Tian, L.
Affiliations
  • University of California, Davis (US)
  • Peking University (CN)
Corresponding author
  • Li, Yulong
  • Tian, Lin
ORCID
Venue
Annual Review of Neuroscience
Publisher
Annual Reviews
Published
2022-07-08
Volume
45
Issue
1
Pages
273-294
ISSN
  • 0147-006X (ISSN-L)
  • 1545-4126
DOI
10.1146/annurev-neuro-110520-031137
PMC
PMC9940643
OpenAlex
W4220839458
Cited by
99
References
11
Citations by year
2026: 19 · 2025: 29 · 2024: 26 · 2023: 20 · 2022: 5
FWCI
3.08
Type
journal article
Language
en
Open access
Locations
  • Annual Review of Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institutes of Health (1uf1mh128336-01)
  • National Science Fund for Distinguished Young Scholars
  • National Institute of Mental Health (R01MH101214, UF1 MH128336, T32MH112507)
  • National Eye Institute (R21EY031858)
  • National Institute of Neurological Disorders and Stroke (U01NS120820, U01NS113295, R01NS129888)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
genetically encoded fluorescent indicators, calcium imaging, neural recording, voltage sensor, drug discovery, behaving animals
MeSH terms
Animals, Brain, Neurons, Receptors, Drug, Neurotransmitter Agents, Optical Imaging

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