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Plant gene editing through de novo induction of meristems
Open accessAbstract
Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, time-consuming, works for only limited species and genotypes, and causes unintended changes to the genome and epigenome. Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem induction. Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants. This induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitted to the next generation. The de novo induction of gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in plant gene editing.
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“Injection of Agrobacterium tumefaciens carrying the development regulators WUS2 and SHOOT MERISTEMLESS (STM) into tobacco seedlings led to the development of gene-edited shoots with heritable mutations (Maher et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Maher et al., 2020
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“De novo induction of geneedited meristems also avoids the tissue culture step and can be induced in aseptically grown seedlings and soil-grown plants (Maher et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Maher et al., 2020
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Cited by
Sorted by citations
Genome engineering for crop improvement and future agriculture
Gao, C. (2021) · Cell · doi:10.1016/j.cell.2021.01.005 · Cited by 1,070
“Developmental regulators can also be used to generate genome-edited dicots through de novo meristem induction, which sidesteps the need for tissue culture (Maher et al., 2020).”
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“A recent study obtained genome editing events by de novo induction of meristems, in which they co-transferred CRISPR/Cas9 systemwith developmental regulators into tobacco plants [33].”
BackgroundNanotechnology to advance CRISPR–Cas genetic engineering of plants
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Metadata
- Authors
- Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Voytas, Daniel F.
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2019-12-16
- Volume
- 38
- Issue
- 1
- Pages
- 84-89
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC6954279
- OpenAlex
- W2995711654
- Cited by
- 541
- References
- 27
- Citations by year
- 2026: 64 · 2025: 86 · 2024: 68 · 2023: 78 · 2022: 94 · 2021: 93 · 2020: 55 · 2019: 2
- FWCI
- 20.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (T32-GM008347)
- Hackett Fund of the University of Minnesota TechAccel
- TechAccel
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Cas9, single guide RNA, somatic cells, dicotyledonous plants, transgenerational inheritance, developmental regulators, epigenome, genome editing
- MeSH terms
- Gene Editing, CRISPR-Associated Protein 9, Base Sequence, Mutation, Plant Proteins, Soil, Nicotiana, Meristem, Plant Shoots, Transgenes, Plants, Genetically Modified, Seedlings
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