The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Reliability of human cortical organoid generation
Open accessAbstract
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
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“Furthermore, several studies have shown that guided cortical organoid differentiation yields highly reproducible organoids as compared to unguided organoid differentiation (72–74).”
From Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems (2025) · cites this paper as [73]
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“A number of groups were able to generate spheroids in chip-based devices171–173, but the spheroids lack spatial complexity and cell types compared to fully-developed organoids.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [173]
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Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
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Individual brain organoids reproducibly form cell diversity of the human cerebral cortex
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Trujillo, C. A., Gao, R., Negraes, P. D., Gu, J., Buchanan, J., Preissl, S., Wang, A., Wu, W., Haddad, G. G., Chaim, I. A., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.08.002 · Cited by 974
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Generation of Functional Human 3D Cortico-Motor Assembloids
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Self-citationMaturation and circuit integration of transplanted human cortical organoids
Revah, O., Gore, F., Kelley, K. W., Andersen, J., Sakai, N., Chen, X., Li, M.-Y., Birey, F., Yang, X., Saw, N. L., et al. (2022) · Nature · doi:10.1038/s41586-022-05277-w · Cited by 504
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Metadata
- Authors
- Yoon, S.-J., Elahi, L. S., Pașca, A. M., Marton, R. M., Gordon, A., Revah, O., Miura, Y., Walczak, E. M., Holdgate, G. M., Fan, H. C., Huguenard, J. R., Geschwind, D. H., & Pașca, S. P.
- Affiliations
- Stanford Medicine (US)
- University of California, Los Angeles (US)
- BD Biosciences (United States) (US)
- Corresponding author
- Pașca, Sergiu P.
- ORCID
- Venue
- Nature Methods
- Published
- 2018-12-20
- Volume
- 16
- Issue
- 1
- Pages
- 75-78
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC6677388
- OpenAlex
- W2904130550
- Cited by
- 507
- References
- 28
- Citations by year
- 2026: 66 · 2025: 74 · 2024: 57 · 2023: 59 · 2022: 67 · 2021: 70 · 2020: 76 · 2019: 35
- FWCI
- 16.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- New York Stem Cell Foundation
- Nature
- National Institutes of Health
- National Institute of Mental Health (R01MH107800, U01MH115745, U01MH115746)
- National Institute of Neurological Disorders and Stroke (R01NS034774)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD000850)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- cortical organoids, human pluripotent stem cells, brain development, xeno-free culture, feeder-free culture, disease modeling
- MeSH terms
- Cell Line, Humans, Organoids, Reproducibility of Results, Prosencephalon, Sequence Analysis, RNA, Tissue Engineering, Pluripotent Stem Cells, Single-Cell Analysis
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