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Cited by 107Zebrafish Biomedical Research ApplicationsAdvanced Fluorescence Microscopy TechniquesNeuroscience and Neuropharmacology Research

Deep three-photon imaging of the brain in intact adult zebrafish

Open access
Chow, Dawnis M., Sinefeld, David, Kolkman, Kristine E., Ouzounov, Dimitre G., Akbari, Najva, Tatarsky, Rose, Bass, Andrew, Xu, Chris +1 more·2020·Nature Methods
DOI 10.1038/s41592-020-0819-7PMC PMC7359951
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Abstract

Behaviors emerge from activity throughout the brain, but noninvasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become noninvasively accessible from embryo to sexually mature adult in a vertebrate model.

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  • The larval condition of the adult skeleton of Danionella, in which the skull roof is missing and the brain is covered only by skin, has led various researchers to turn to Danionella as the adult vertebrate model organism to study neurophysiological questions by deep imaging their brain activity in vivo.

    “It is this larval condition of their adult skeleton, in which the skull roof is missing and the brain is covered only by skin, that has led various researchers to turn to Danionella as the adult vertebrate model organism to study neurophysiological questions by deep imaging their brain activity in vivo11–14.”

    From The emerging vertebrate model species for neurophysiological studies is Danionella cerebrum, new species (Teleostei: Cyprinidae) (2021) · cites this paper as [13]

    Split into 2 atoms — 0 confirmed, 1 partially supported, 0 disputed, 0 misattributed, 1 unverifiable. Expand the claim to see per-atom verdicts.

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  • The miniature adult size and larval appearance of Danionella, combined with a diverse behavioral repertoire linked to sound production by males, have established Danionella as an important model for neurophysiological studies.

    “The miniature adult size and larval appearance of Danionella, combined with a diverse behavioral repertoire linked to sound production by males, have established Danionella as an important model for neurophysiological studies.”

    From The emerging vertebrate model species for neurophysiological studies is Danionella cerebrum, new species (Teleostei: Cyprinidae) (2021) · cites this paper as [13]

    The paper describes Danionella as a small, translucent, emerging fish model and reports imaging the brains of intact adults (Abstract; Online Methods, Structural Imaging; pages not provided). It does not describe a larval appearance, male sound production, or a diverse behavioral repertoire, and it does not say those traits established Danionella as a neurophysiological model. (LLM, conf=0.87)

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Cites

Flagged first, then by citations

Research works printed but not linked: 17

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  10. [13]Wang T Three-photon imaging of mouse brain structure and function through the intact skull. Nat Methods 15, 789– 792, doi: 10.1038/s41592-018-0115-y (2018). 30202059 PMC6188644 · DOI
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  13. [16]Satou C, Kimura Y & Higashijima S Generation of multiple classes of V0 neurons in zebrafish spinal cord: progenitor heterogeneity and temporal control of neuronal diversity. J Neurosci 32, 1771– 1783, doi: 10.1523/JNEUROSCI.5500-11.2012 (2012). 22302816 PMC6703360 · DOI
  14. [17]Bae Y-K Anatomy of zebrafish cerebellum and screen for mutations affecting its development. Developmental Biology 330, 406– 426, doi: 10.1016/j.ydbio.2009.04.013 (2009). 19371731 · DOI · Paper page
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  17. [20]Britz R, Conway KW & Ruber L Spectacular morphological novelty in a miniature cyprinid fish, Danionella dracula n. sp. Proc Biol Sci 276, 2179– 2186, doi: 10.1098/rspb.2009.0141 (2009). 19324738 PMC2677611 · DOI · Paper page

Cited by

Sorted by citations

Authors
Chow, D. M., Sinefeld, D., Kolkman, K. E., Ouzounov, D. G., Akbari, N., Tatarsky, R., Bass, A., Xu, C., & Fetcho, J. R.
Affiliations
Cornell University (US)
Corresponding author
  • Xu, Chris
  • Fetcho, Joseph R.
ORCID
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2020-04-27
Volume
17
Issue
6
Pages
605-608
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/s41592-020-0819-7
PMC
PMC7359951
OpenAlex
W3019174158
Cited by
107
References
3
Citations by year
2026: 9 · 2025: 16 · 2024: 26 · 2023: 16 · 2022: 17 · 2021: 13 · 2020: 10
FWCI
6.92
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation (DBI-1707312, IOS-1457108)
  • U.S. Department of Health & Human Services | NIH | Center for Scientific Review (1U01NS103516, IOS-1457108)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
three-photon microscopy, zebrafish brain, deep brain imaging, non-invasive optical imaging, telencephalon, cerebellum, optic tectum, adult zebrafish, brain structure and function
MeSH terms
Animals, Cerebellum, Superior Colliculi, Telencephalon, Zebrafish, Photons, Imaging, Three-Dimensional

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