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A chromosome-level genome assembly for the astaxanthin-producing microalga Haematococcus pluvialis

Bian, Chao, Liu, Chenglong, Zhang, Guiying, Tao, Ming, Huang, Danqiong, Wang, Chaogang, Lou, Sulin, Li, Hui, Shi, Qiong, Hu, Zhangli·2023·Scientific Data
DOI 10.1038/s41597-023-02427-1PMC PMC10400597
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Abstract

The green microalga Haematococcus pluvialis can synthesize high amounts of astaxanthin, which is a valuable antioxidant that has been utilized in human health, cosmetics, and aquaculture. To illustrate detailed molecular clues to astaxanthin yield, we performed PacBio HIFI along with Hi-C sequencing to construct an improved chromosome-level haplotypic genome assembly with 32 chromosomes and a genome size of 316.0 Mb. Its scaffold N50 (942.6 kb) and contig N50 (304.8 kb) have been upgraded remarkably from our previous genome draft, and a total of 32,416 protein-coding genes were predicted. We also established a high-evidence phylogenetic tree from seven representative algae species, with the main aim to calculate their divergence times and identify expanded/contracted gene families. We also characterized genome-wide localizations on chromosomes of some important genes such as five BKTs (encoding beta-carotene ketolases) that are putatively involved in astaxanthin production. In summary, we reported the first chromosome-scale map of H. pluvialis , which provides a valuable genetic resource for in-depth biomedical investigations on this momentous green alga and commercial astaxanthin bioproduction.

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  • The authors constructed the first complete telomere-to-telomere (T2T) chromosome-scale genome assembly of X-ray tetra by integrating PacBio HiFi, ONT ultra-long, and Hi-C sequencing technologies.

    “we constructed the first complete telomere-to-telomere (T2T) chromosome-scale genome assembly of the X-ray tetra by integration of PacBio HiFi, ONT ultra-long, and Hi-C sequencing technologies.”

    From A complete telomere-to-telomere chromosome-level genome assembly of X-ray tetra (Pristella maxillaris) (2025) · cites this paper as [11]

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Research works printed but not linked: 38

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Metadata

Authors
Bian, C., Liu, C., Zhang, G., Tao, M., Huang, D., Wang, C., Lou, S., Li, H., Shi, Q., & Hu, Z.
Venue
Scientific Data
Publisher
Springer Science and Business Media LLC
Published
2023-08-03
Volume
10
Issue
1
ISSN
2052-4463
DOI
10.1038/s41597-023-02427-1
PMC
PMC10400597
Cited by
26
References
1
Language
en
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