The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Generation of mutation hotspots in ageing bacterial colonies
Open accessPublished version of a preprint
Abstract
How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
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Metadata
- Authors
- Sekowska, A., Wendel, S., Fischer, E. C., Nørholm, M. H. H., & Danchin, A.
- Venue
- Scientific Reports
- Published
- 2016-12-05
- Volume
- 6
- Issue
- 1
- Pages
- 2-2
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2952699447
- Cited by
- 320
- References
- 25
- FWCI
- 19.08
- Type
- journal article
- Language
- en
- Open access
- gold
- Licence
- creativecommons.org/licenses/by/4.0 (tdm)
- creativecommons.org/licenses/by/4.0 (vor)
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