Cited by 124Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyMetabolomics and Mass Spectrometry Studies

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Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers

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Artegoitia, Virginia M., Foote, Andrew P., Lewis, Ronald M., Freetly, Harvey C.·2017·Scientific Reports

DOI 10.1038/s41598-017-02856-0PMC PMC5460109

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Abstract

The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before slaughter and at slaughter, respectively. As a result of the metabolomics analysis of rumen fluid, 33 metabolites differed between the ADG groups based on t-test, fold changes and partial least square discriminant analysis. These metabolites were primarily involved in linoleic and alpha-linolenic metabolism (impact-value 1.0 and 0.75, respectively; P < 0.05); both pathways were down-regulated in the greatest-ADG compared with least-ADG group. Ruminal biohydrogenation might be associated with the overall animal production. The fatty acids were quantified in rumen and plasma using targeted MS to validate and evaluate the simple combination of metabolites that effectively predict ADG.

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Figure 1 Ruminal metabolomic profile of the steers with the greatest average daily gain (high-ADG; open red triangle) and the least ADG (low-ADG; green plus) with similar average dry matter intake. (A) Principal component analysis for UPLC-qTOF 90 metabolites identified to differ between ADG by t-test (P < 0.1). (B) Partial Least Square-Discriminant Analysis for UPLC-qTOF 90 metabolites identified to differ between ADG by t-test (P < 0.1). One data point represents one steer.CC BY · PubMed Central

Figure 2 Ruminal metabolomics pathway analysis by MetaboAnalyst 3.0 Software on the steers with the greatest average daily gain compare to the least ADG with similar average dry matter intake according to Bos taurus KEGG pathway database. UPLC-qTOF 90 metabolites identified to differ between ADG by t-test (P < 0.1). (a) Alpha-linolenic acid metabolism, (b) linoleic metabolism (c) taurine and hypotaurine metabolism d) phenylalanine, tyrosine and tryptophan (e) sphingolipids (f) phenylalanine (g) retinol (h) histidine (i) primary bile biosynthesis (j) glycerophospholipid (k) tyrosin (l) porphyrin and chlorophyll (m) steroid hormone biosynthesis (n) glutathione (o) Riboflavin (p) Gluconeogenesis. The darker the color and larger the size represent higher P-value from enrichment analysis and greater impact from the pathway topology analysis, respectively.CC BY · PubMed Central

Figure 3 The integrative ruminal metabolic pathway changes on the steers with the greatest average daily gain compare to the least ADG with similar average dry matter intake according to Bos taurus KEGG pathway database. UPLC-qTOF 90 metabolites identified to differ between ADG by t-test (P < 0.1).CC BY · PubMed Central

Figure 4 Receiver operating curves (ROC) of fatty acids for the differential identification of feed efficiency on steers. (A) Rumen fluid ROC using the combination of pentadecanoic acid, palmitic acid, linoleic acid and alpha-linolenic acid. (B) Plasma ROC using the combination of arachidonic acid/docosahexanoic acid ratio and alpha-linolenic acid.CC BY · PubMed Central

Figure 5 Overview of workflow utilized in the identification of biomarkers of feed efficiency on crossbreed steers.CC BY · PubMed Central

Figure 6 Rumen sampling selection. The two animals groups were selected by the greatest average daily gain (high-ADG; open red triangle; n = 8) and the least ADG steers (low-ADG; green plus; n = 8) with similar average dry matter intake (dash line) from the total population (black dots; n = 144) evaluated.CC BY · PubMed Central

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Additional file 8 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751493
Additional file 5 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751485
Additional file 10 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751499
Additional file 3 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751479
Additional file 17 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751520
Additional file 13 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751508
Additional file 6 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751488
Additional file 7 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751491
Additional file 16 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751517
Additional file 20 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751529
Additional file 2 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751476
Additional file 14 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751511
Additional file 12 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751505
Additional file 15 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., Bi, Y.-L., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751514
Additional file 18 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751523
Additional file 19 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751526
Additional file 1 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Xu, S.-Y., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751473
Additional file 11 of Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
Shengyang, X. U., Feng, X.-R., Zhao, W., YanLiang, B., Diao, Q.-Y., & Tu, Y. (2024) · Figshare · doi:10.6084/m9.figshare.26751502
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Authors: Artegoitia, V. M., Foote, A. P., Lewis, R. M., & Freetly, H. C.
Affiliations: University of Nebraska–Lincoln (US)
Roman L. Hruska U.S. Meat Animal Research Center (US)
Corresponding author: Freetly, Harvey C.
ORCID: Artegoitia, Virginia M.
Foote, Andrew P.
Lewis, Ronald M.
Venue: Scientific Reports
Publisher: Springer Science and Business Media LLC
Published: 2017-06-06
Volume: 7
Issue: 1
Pages: 2864-2864
ISSN: 2045-2322 (ISSN-L)
DOI: 10.1038/s41598-017-02856-0
PMC: PMC5460109
OpenAlex: W2553397641
Cited by: 123
References: 37
Citations by year: 2026: 7 · 2025: 4 · 2024: 32 · 2023: 15 · 2022: 15 · 2021: 16 · 2020: 15 · 2019: 7 · 2018: 11
FWCI: 15.70
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0 (tdm)
creativecommons.org/licenses/by/4.0 (vor)
Locations: Scientific Reports (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, other-oa)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Lincoln (University of Nebraska) (submittedVersion)
Lincoln (University of Nebraska) (submittedVersion, cc-by)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: U.S. Department of Agriculture
Topics: Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords: feed efficiency, average daily gain, crossbred steers, high-concentrate diet, linoleic acid metabolism, alpha-linolenic acid metabolism, ruminal biohydrogenation, fatty acids, rumen metabolites, growth performance prediction
MeSH terms: Animals, Animal Feed, Breeding, Cattle, Crosses, Genetic, Fatty Acids, ROC Curve, Rumen, Extracellular Fluid, Metabolic Networks and Pathways, Metabolomics, Metabolome

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Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers

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