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Systematic analysis of gene expression patterns associated with postmortem interval in human tissues
Open accessAbstract
Postmortem mRNA degradation is considered to be the major concern in gene expression research utilizing human postmortem tissues. A key factor in this process is the postmortem interval (PMI), which is defined as the interval between death and sample collection. However, global patterns of postmortem mRNA degradation at individual gene levels across diverse human tissues remain largely unknown. In this study, we performed a systematic analysis of alteration of gene expression associated with PMI in human tissues. From the Genotype-Tissue Expression (GTEx) database, we evaluated gene expression levels of 2,016 high-quality postmortem samples from 316 donors of European descent, with PMI ranging from 1 to 27 hours. We found that PMI-related mRNA degradation is tissue-specific, gene-specific, and even genotype-dependent, thus drawing a more comprehensive picture of PMI-associated gene expression across diverse human tissues. Additionally, we also identified 266 differentially variable (DV) genes, such as DEFB4B and IFNG, whose expression is significantly dispersed between short PMI (S-PMI) and long PMI (L-PMI) groups. In summary, our analyses provide a comprehensive profile of PMI-associated gene expression, which will help interpret gene expression patterns in the evaluation of postmortem tissues.
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Metadata
- Authors
- Zhu, Y., Wang, L., Yin, Y., & Yang, E.
- Affiliations
- Peking University (CN)
- Corresponding author
- Yin, Yuxin
- Yang, Ence
- Venue
- Scientific Reports
- Published
- 2017-07-14
- Volume
- 7
- Issue
- 1
- Pages
- 5435-5435
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5511187
- OpenAlex
- W2734938713
- Cited by
- 129
- References
- 46
- Citations by year
- 2026: 12 · 2025: 15 · 2024: 17 · 2023: 9 · 2022: 19 · 2021: 21 · 2020: 13 · 2019: 15 · 2018: 8
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (111 Project, 31401132, B07001)
- Peking University
- Higher Education Discipline Innovation Project (B07001)
- National Key Research and Development Program of China (#2016YFA0500302)
- Peking University Health Science Center
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- postmortem interval, gene expression, tissue-specific gene expression, genotype-dependent gene expression, interferon-gamma, GTEx
- MeSH terms
- Autopsy, Cadaver, Genotype, Humans, Interferon-gamma, Organ Specificity, Postmortem Changes, RNA, Messenger, Time Factors, Gene Expression, Databases, Factual, Gene Expression Profiling, RNA Stability, beta-Defensins, White People
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