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Cited by 40Peroxisome Proliferator-Activated ReceptorsAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic Diseases

Ndrg1 promotes adipocyte differentiation and sustains their function

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Cai, Kai, El-Merahbi, Rabih, Loeffler, Mona, Mayer, Alexander E., Sumara, Grzegorz·2017·Scientific Reports
DOI 10.1038/s41598-017-07497-xPMC PMC5543145
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Abstract

Adipocytes play a central role in maintaining metabolic homeostasis in the body. Differentiation of adipocyte precursor cells requires the transcriptional activity of peroxisome proliferator-activated receptor-γ (Pparγ) and CCAAT/enhancer binding proteins (C/Ebps). Transcriptional activity is regulated by signaling modules activated by a plethora of hormones and nutrients. Mechanistic target of rapamacin complexes (mTORC) 1 and 2 are central for the coordination of hormonal and nutritional inputs in cells and are essential for adipogenesis. Serum glucocorticoid kinase 1 (Sgk1)-dependent phosphorylation of N-Myc downstream-regulated gene 1 (Ndrg1) is a hallmark of mTORC2 activation in cells. Moreover, Pparγ activation promotes Ndrg1 expression. However, the impact of Ndrg1 on adipocyte differentiation and function has not yet been defined. Here, we show that Ndrg1 expression and its Sgk1-dependent phosphorylation are induced during adipogenesis. Consistently, we demonstrate that Ndrg1 promotes adipocyte differentiation and function by inducing Pparγ expression. Additionally, our results indicate that Ndrg1 is required for C/Ebpα phosphorylation. Moreover, we found that Ndrg1 phosphorylation by Sgk1 promotes adipocyte formation. Taken together, we show that induction of Ndrg1 expression by Pparγ and its phosphorylation by Sgk1 kinase are required for the acquisition of adipocyte characteristics by precursor cells.

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Cited by

Sorted by citations

Authors
Cai, K., El-Merahbi, R., Loeffler, M., Mayer, A. E., & Sumara, G.
Affiliations
University of Würzburg (DE)
Corresponding author
Sumara, Grzegorz
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-08-03
Volume
7
Issue
1
Pages
7191-7191
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-07497-x
PMC
PMC5543145
OpenAlex
W2741899374
Cited by
40
References
1
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 4 · 2018: 2
FWCI
1.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Online Publication Service of Würzburg University (Würzburg University) (submittedVersion, cc-by)
Funders
Deutsche Forschungsgemeinschaft (Su 820/1-1, 820/1-1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
NDRG1, adipogenesis, adipocyte function, PPARγ, SGK1, mTORC2, protein phosphorylation, adipocyte precursors, transcriptional regulation
MeSH terms
Serum-Glucocorticoid Regulated Kinases, N-myc Downstream-Regulated Gene 1 Protein, Animals, Cell Differentiation, Immunohistochemistry, Mutation, Phosphorylation, Gene Expression, Protein Serine-Threonine Kinases, Adipocytes, Immediate-Early Proteins, Cell Cycle Proteins, CCAAT-Enhancer-Binding Protein-alpha, RNA, Small Interfering, 3T3-L1 Cells, PPAR gamma, Intracellular Signaling Peptides and Proteins, Adipogenesis, Lipid Metabolism, Mice, Gene Knockdown Techniques

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