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Cited by 20Geomagnetism and Paleomagnetism StudiesMagnetic Properties and Synthesis of FerritesMicrobial Fuel Cells and Bioremediation

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Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria

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Werckmann, Jacques, Cypriano, Jefferson, Lefèvre, Christopher T., Dembelé, Kassiogé, Ersen, Ovidiu, Bazylinski, Dennis A., Lins, Ulysses, Farina, Marcos·2017·Scientific Reports
DOI 10.1038/s41598-017-08994-9PMC PMC5557804
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Abstract

Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here we address the question: can iron transported inside MTB for the production of magnetite crystals be spatially mapped using electron microscopy? Cultured and uncultured MTB from brackish and freshwater lagoons were studied using analytical transmission electron microscopy in an attempt to answer this question. Scanning transmission electron microscopy was used at sub-nanometric resolution to determine the distribution of elements by implementing high sensitivity energy dispersive X-ray (EDS) mapping and electron energy loss spectroscopy (EELS). EDS mapping showed that magnetosomes are enmeshed in a magnetosomal matrix in which iron accumulates close to the magnetosome forming a continuous layer visually appearing as a corona. EELS, obtained at high spatial resolution, confirmed that iron was present close to and inside the lipid bilayer magnetosome membrane. This study provides important clues to magnetite formation in MTB through the discovery of a mechanism where iron ions accumulate prior to magnetite biomineralization.

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    “This biomineralization also occurs within intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane [139].”
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Metadata

Authors
Werckmann, J., Cypriano, J., Lefèvre, C. T., Dembelé, K., Ersen, O., Bazylinski, D. A., Lins, U., & Farina, M.
Affiliations
  • Universidade Federal do Rio de Janeiro (BR)
  • Centre National de la Recherche Scientifique (FR)
  • University of Nevada, Las Vegas (US)
Corresponding author
Werckmann, Jacques
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-08-15
Volume
7
Issue
1
Pages
8291-8291
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-08994-9
PMC
PMC5557804
OpenAlex
W2744758543
Cited by
20
References
1
Citations by year
2025: 2 · 2024: 5 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3
FWCI
0.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • Digital Scholarship - UNLV (University of Nevada Reno) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (1423939)
  • Agence Nationale de la Recherche (ANR-16-TERC-0025)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
  • Centre National de la Recherche Scientifique
  • Universidade Federal do Rio de Janeiro
Topics
  • Physical Sciences › Earth and Planetary Sciences › Geophysics › Geomagnetism and Paleomagnetism Studies
  • Physical Sciences › Materials Science › Materials Chemistry › Magnetic Properties and Synthesis of Ferrites
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
Keywords
magnetotactic bacteria, magnetite biomineralization, magnetosomes, iron accumulation, crystal nucleation and growth, iron transport, electron energy loss spectroscopy, energy-dispersive X-ray mapping
MeSH terms
Crystallization, Iron, Rhodospirillaceae, Liquid Crystals, Ferrosoferric Oxide, Magnetosomes

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