A novel quantification-driven proteomic strategy identifies an endogenous peptide of pleiotrophin as a new biomarker of Alzheimer’s disease
Open accessAbstract
We present a new, quantification-driven proteomic approach to identifying biomarkers. In contrast to the identification-driven approach, limited in scope to peptides that are identified by database searching in the first step, all MS data are considered to select biomarker candidates. The endopeptidome of cerebrospinal fluid from 40 Alzheimer’s disease (AD) patients, 40 subjects with mild cognitive impairment, and 40 controls with subjective cognitive decline was analyzed using multiplex isobaric labeling. Spectral clustering was used to match MS/MS spectra. The top biomarker candidate cluster (215% higher in AD compared to controls, area under ROC curve = 0.96) was identified as a fragment of pleiotrophin located near the protein’s C-terminus. Analysis of another cohort (n = 60 over four clinical groups) verified that the biomarker was increased in AD patients while no change in controls, Parkinson’s disease or progressive supranuclear palsy was observed. The identification of the novel biomarker pleiotrophin 151–166 demonstrates that our quantification-driven proteomic approach is a promising method for biomarker discovery, which may be universally applicable in clinical proteomics.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Elevated PTN is detected in the substantia nigra of patients with Parkinson's disease and in the CSF of patients with AD.
“Elevated PTN is detected in the substantia nigra of patients with Parkinson's disease and in the cerebrospinal fluid (CSF) of patients with AD. 41 , 42”
From Loss of <i>Inpp5d</i> has disease‐relevant and sex‐specific effects on glial transcriptomes (2024) · cites this paper as [42]
The Results (Validation of PTN 151–166 as a biomarker of AD; page unavailable) report elevated CSF levels in AD patients, supporting the AD portion of the sentence. The measured substance was the PTN 151–166 peptide, not full-length PTN; the paper does not establish elevated PTN in the substantia nigra of patients with Parkinson’s disease. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
R: A Language and Environment for Statistical Computing
R Core Team (2000) · doi:10.32614/r.manuals · Cited by 355,706 · see citing works
McKhann, G. M., Knopman, D. S., Chertkow, H., Hyman, B. T., Jack, C. R., Kawas, C. H., Klunk, W. E., Koroshetz, W. J., Manly, J. J., Mayeux, R., et al. (2011) · Alzheimer's & Dementia · doi:10.1016/j.jalz.2011.03.005 · Cited by 18,657 · see citing works
Self-citationParkinsonism: onset, progression, and mortality
Hoehn, M. M., & Yahr, M. D. (1967) · Neurology · doi:10.1212/wnl.17.5.427 · Cited by 12,107 · see citing works
Mild Cognitive Impairment: Clinical Characterization and Outcome
Petersen, R. C., Smith, G. E., Waring, S. C., Ivnik, R. J., Tangalos, E. G., & Kokmen, E. (1999) · Archives of Neurology · doi:10.1001/archneur.56.3.303 · Cited by 8,980 · see citing works
Eng, J. K., McCormack, A. L., & Yates, J. R. (1994) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(94)80016-2 · Cited by 6,484 · see citing works
Overdependence on degraded gist memory in Alzheimer's disease.
Gallo, D. A., Shahid, K. R., Olson, M. A., Solomon, T. M., Schacter, D. L., & Budson, A. E. (2006) · Neuropsychology · doi:10.1037/0894-4105.20.6.625 · Cited by 4,756 · see citing works
Advancing research diagnostic criteria for Alzheimer's disease: the IWG-2 criteria
Dubois, B., Feldman, H. H., Jacova, C., Hampel, H., Molinuevo, J. L., Blennow, K., DeKosky, S. T., Gauthier, S., Selkoe, D., Bateman, R., et al. (2014) · The Lancet Neurology · doi:10.1016/s1474-4422(14)70090-0 · Cited by 3,321 · see citing works
Self-citationDiagnostic Criteria for Parkinson Disease
Gelb, D. J., Oliver, E., & Gilman, S. (1999) · Archives of Neurology · doi:10.1001/archneur.56.1.33 · Cited by 2,822 · see citing works
Mild Cognitive Impairment: Ten Years Later
Petersen, R. C., Roberts, R. O., Knopman, D. S., Boeve, B. F., Geda, Y. E., Ivnik, R. J., Smith, G. E., & Jack, C. R. (2009) · Archives of Neurology · doi:10.1001/archneurol.2009.266 · Cited by 1,447 · see citing works
Mobile and localized protons: a framework for understanding peptide dissociation
Wysocki, V. H., Tsaprailis, G., Smith, L. L., & Breci, L. A. (2000) · Journal of Mass Spectrometry · doi:10.1002/1096-9888(200012)35:12 · Cited by 998 · see citing works
(2026) · Molecular Medicine Reports · doi:10.3892/mmr · Cited by 920 · see citing works
A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking
Teunissen, C. E., Petzold, A., Bennett, J. L., Berven, F. S., Brundin, L., Comabella, M., Franciotta, D., Frederiksen, J. L., Fleming, J. O., Furlan, R., et al. (2009) · Neurology · doi:10.1212/wnl.0b013e3181c47cc2 · Cited by 845 · see citing works
Self-citationIncreasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses
McAlister, G. C., Huttlin, E. L., Haas, W., Ting, L., Jedrychowski, M. P., Rogers, J. C., Kuhn, K., Pike, I., Grothe, R. A., Blethrow, J. D., et al. (2012) · Analytical Chemistry · doi:10.1021/ac301572t · Cited by 605 · see citing works
Shevchenko, A., Sunyaev, S. R., Loboda, A., Shevchenko, A., Peer Bork, B., Ens, W., & Standing, K. G. (2001) · Analytical Chemistry · doi:10.1021/ac0013709 · Cited by 580 · see citing works
Dayon, L., Hainard, A., Licker, V., Turck, N., Kuhn, K., Hochstrasser, D. F., Burkhard, P. R., & Sanchez, J. (2008) · Analytical Chemistry · doi:10.1021/ac702422x · Cited by 565 · see citing works
Clinical utility of cerebrospinal fluid biomarkers in the diagnosis of early Alzheimer's disease
Blennow, K., Dubois, B., Fagan, A. M., Lewczuk, P., de Leon, M. J., & Hampel, H. J. (2014) · Alzheimer s & Dementia · doi:10.1016/j.jalz.2014.02.004 · Cited by 455 · see citing works
Self-citationMeng, K., Rodríguez‐Peña, A., Dimitrov, T., Chen, W., Yamin, M., Noda, M., & Deuel, T. F. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.020487997 · Cited by 414 · see citing works
Optimizing Patient Care and Research: The Amsterdam Dementia Cohort
van der Flier, W. M., Pijnenburg, Y. A. L., Prins, N. D., Lemstra, A. W., Bouwman, F. H., Teunissen, C. E., van Berckel, B. N. M., Stam, C. J., Barkhof, F., Visser, P. J., et al. (2014) · Journal of Alzheimer s Disease · doi:10.3233/jad-132306 · Cited by 390 · see citing works
Self-citation<i>APOE</i> ε4: The most prevalent yet understudied risk factor for Alzheimer's disease
Michaelson, D. M. (2014) · Alzheimer's & Dementia · doi:10.1016/j.jalz.2014.06.015 · Cited by 331 · see citing works
Frese, C. K., Altelaar, M. A. F., Hennrich, M. L., Nolting, D., Zeller, M., Griep‐Raming, J., Heck, A. J. R., & Mohammed, S. (2011) · Journal of Proteome Research · doi:10.1021/pr1011729 · Cited by 314 · see citing works
mMass as a Software Tool for the Annotation of Cyclic Peptide Tandem Mass Spectra
Niedermeyer, T. H. J., & Strohalm, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0044913 · Cited by 304 · see citing works
Frese, C. K., Altelaar, M. A. F., van den Toorn, H., Nolting, D., Griep‐Raming, J., Heck, A. J. R., & Mohammed, S. (2012) · Analytical Chemistry · doi:10.1021/ac3025366 · Cited by 300 · see citing works
Raulo, E., Chernousov, M. A., Carey, D., Nolo, R. M., & Rauvala, H. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99975-8 · Cited by 284 · see citing works
Maeda, N., Nishiwaki, T., Shintani, T., Hamanaka, H., & Noda, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.35.21446 · Cited by 283 · see citing works
Clustering Millions of Tandem Mass Spectra
Frank, A. M., Bandeira, N., Shen, Z., Tanner, S. W., Briggs, S. P., Smith, R. D., & Pevzner, P. A. (2007) · Journal of Proteome Research · doi:10.1021/pr070361e · Cited by 267 · see citing works
Improving large‐scale proteomics by clustering of mass spectrometry data
Beer, I., Barnea, E., Ziv, T., & Admon, A. (2004) · PROTEOMICS · doi:10.1002/pmic.200300652 · Cited by 183 · see citing works
Chondroitin Sulfate Proteoglycans Are Associated with the Lesions of Alzheimer's Disease
DeWitt, D. A., Silver, J., Canning, D. R., & Perry, G. L. (1993) · Experimental Neurology · doi:10.1006/exnr.1993.1081 · Cited by 182 · see citing works
Tabb, D. L., MacCoss, M. J., Wu, C. C., Anderson, S., & Yates, J. R. (2003) · Analytical Chemistry · doi:10.1021/ac026424o · Cited by 163 · see citing works
Accurate Multiplexed Proteomics at the MS2 Level Using the Complement Reporter Ion Cluster
Wühr, M., Haas, W., McAlister, G. C., Peshkin, L., Rad, R., Kirschner, M. W., & Gygi, S. P. (2012) · Analytical Chemistry · doi:10.1021/ac301962s · Cited by 147 · see citing works
Himburg, H. A., Harris, J. R., Ito, T., Daher, P., Russell, J. L., Quarmyne, M., Doan, P. L., Helms, K. L., Nakamura, M., Fixsen, E., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.002 · Cited by 147 · see citing works
Kim, J., Yoon, H., Basak, J., & Kim, J. (2014) · Molecules and Cells · doi:10.14348/molcells.2014.0248 · Cited by 141 · see citing works
Yeh, H.-J., He, Y. Y., Xu, J., Hsu, C. Y., & Deuel, T. F. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-10-03699.1998 · Cited by 141 · see citing works
Differential Induction of Glioblastoma Migration and Growth by Two Forms of Pleiotrophin
Lu, K. V., Jong, K. A., Kim, G., Singh, J., Dia, E. Q., Yoshimoto, K., Wang, M. Y., Cloughesy, T. F., Nelson, S. F., & Mischel, P. S. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m502614200 · Cited by 131 · see citing works
Comi, C., Carecchio, M., Chiocchetti, A., Nicola, S., Galimberti, D., Fenoglio, C., Cappellano, G., Monaco, F., Scarpini, E., & Dianzani, U. (2010) · Journal of Alzheimer's Disease · doi:10.3233/jad-2010-1309 · Cited by 120 · see citing works
Hölttä, M., Minthon, L., Hansson, O., Holmén-Larsson, J., Pike, I., Ward, M., Kuhn, K., Rüetschi, U., Zetterberg, H., Blennow, K., et al. (2015) · Journal of Proteome Research · doi:10.1021/pr501076j · Cited by 100 · see citing works
Self-citationPleiotrophin as a central nervous system neuromodulator, evidences from the hippocampus
González-Castillo, C., Ortuño‐Sahagún, D., Guzmán-Brambila, C., Pallàs, M., & Rojas-Mayorquín, A. E. (2015) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00443 · Cited by 97 · see citing works
Mass spectrometry for translational proteomics: progress and clinical implications
Baker, E. S., Liu, T., Petyuk, V., Burnum-Johnson, K., Ibrahim, Y., Anderson, G., & Smith, R. D. (2012) · Genome Medicine · doi:10.1186/gm364 · Cited by 88 · see citing works
Wanaka, A., Carroll, S. L., & Milbrandt, J. (1993) · Developmental Brain Research · doi:10.1016/0165-3806(93)90166-8 · Cited by 85 · see citing works
Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease
Khoonsari, P. E., Månberg, A., Lönnberg, M., Mikus, M. S., Kilander, L., Lannfelt, L., Bergquist, J., Ingelsson, M., Nilsson, P., Kultima, K., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0150672 · Cited by 84 · see citing works
Mikelis, C. M., Sfaelou, E., Κουτσιούμπα, Μ., Kieffer, N., & Papadimitriou, Ε. (2009) · The FASEB Journal · doi:10.1096/fj.08-117564 · Cited by 81 · see citing works
A potential endophenotype for Alzheimer's disease: cerebrospinal fluid clusterin
Deming, Y., Xia, J., Cai, Y., Lord, J., Holmans, P., Bertelsen, S., Holtzman, D., Morris, J. C., Bales, K., Pickering, E. H., et al. (2016) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2015.09.009 · Cited by 66 · see citing works
Mathivet, T., Mazot, P., & Vigny, M. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2007.07.011 · Cited by 65 · see citing works
MS2Grouper: Group assessment and synthetic replacement of duplicate proteomic tandem mass spectra
Tabb, D. L., Thompson, M. R., Khalsa-Moyers, G., VerBerkmoes, N. C., & McDonald, W. H. (2005) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2005.04.010 · Cited by 64 · see citing works
Elevated Osteopontin Levels in Mild Cognitive Impairment and Alzheimer's Disease
Sun, Y., Yin, X.-S., Guo, H., Han, R.-K., He, R.-D., & Chi, L. (2013) · Mediators of Inflammation · doi:10.1155/2013/615745 · Cited by 63 · see citing works
Synaptic Proteins in Alzheimer's Disease
Marksteiner, J., Kaufman, W. A., Gurka, P., & Humpel, C. (2002) · Journal of Molecular Neuroscience · doi:10.1385/jmn:18:1-2:53 · Cited by 54 · see citing works
Explorative and targeted neuroproteomics in Alzheimer's disease
Brinkmalm, A., Portelius, E., Öhrfelt, A., Brinkmalm, G., Andréasson, U., Gobom, J., Blennow, K., & Zetterberg, H. (2015) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2015.01.009 · Cited by 45 · see citing works
Self-citationMarksteiner, J., Lechner, T., Kaufmann, W. A., Gurka, P., Humpel, C., Nowakowski, C., Maier, H., & Jellinger, K. A. (2000) · Acta Neuropathologica · doi:10.1007/s004010000239 · Cited by 44 · see citing works
Bernard‐Pierrot, I., Delbé, J., Caruelle, D., Barritault, D., Courty, J., & Milhiet, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m010913200 · Cited by 40 · see citing works
Glycosylation of Human Plasma Clusterin Yields a Novel Candidate Biomarker of Alzheimer’s Disease
Liang, H., Russell, C., Mitra, V., Chung, R., Hye, A., Bazenet, C. E., Lovestone, S., Pike, I., & Ward, M. A. (2015) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00892 · Cited by 37 · see citing works
Heparin affin regulatory peptide is a key player in prostate cancer cell growth and angiogenicity
Hatziapostolou, M., Delbé, J., Katsoris, P., Polytarchou, C., Courty, J., & Papadimitriou, Ε. (2005) · The Prostate · doi:10.1002/pros.20270 · Cited by 37 · see citing works
Clinical proteomics: Current status, challenges, and future perspectives
Chiou, S., & Wu, C. (2011) · The Kaohsiung Journal of Medical Sciences · doi:10.1016/j.kjms.2010.12.001 · Cited by 37 · see citing works
Activity-induced enhancement of HB-GAM expression in rat hippocampal slices
Lauri, S. E., Taira, T., Kaila, K., & Rauvala, H. (1996) · Neuroreport · doi:10.1097/00001756-199607080-00029 · Cited by 36 · see citing works
Novel cerebrospinal fluid biomarkers of axonal degeneration in frontotemporal dementia.
Mattsson, N., Rüetschi, U., Pijnenburg, Y., Blankenstein, M., Podust, V., Li, S., Fagerberg, I., Rosengren, L., Blennow, K., & Zetterberg, H. (2008) · Molecular Medicine Reports · doi:10.3892/mmr_00000025 · Cited by 34 · see citing works
Ryan, E., Shen, D., & Wang, X. (2016) · FEBS Journal · doi:10.1111/febs.13686 · Cited by 33 · see citing works
Mikelis, C. M., Λάμπρου, Μ., Κουτσιούμπα, Μ., Koutsioubas, A. G., Spyranti, Z., Zompra, A. A., Spiliopoulos, N., Vradis, A., Katsoris, P., Spyroulias, G. A., et al. (2011) · Journal of Cellular Biochemistry · doi:10.1002/jcb.23066 · Cited by 29 · see citing works
Converging Pathways of Chromogranin and Amyloid Metabolism in the Brain
Mattsson, N., Johansson, P., Hansson, O., Wallin, A., Johansson, J.-O., Andréasson, U., Andersen, O., Haghighi, S., Olsson, M., Stridsberg, M., et al. (2010) · Journal of Alzheimer s Disease · doi:10.3233/jad-2010-091651 · Cited by 20 · see citing works
Self-citationCyclin-dependent kinase 5 mediates pleiotrophin-induced endothelial cell migration
Lampropoulou, E., Logoviti, I., Κουτσιούμπα, Μ., Hatziapostolou, M., Polytarchou, C., Skandalis, S. S., Hellman, U., Fousteris, M. A., Nikolaropoulos, S., Choleva, E., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-24326-x · Cited by 19 · see citing works
Schmidt, M. F., Atkinson, P. B., & Kater, S. B. (1996) · Journal of Neurobiology · doi:10.1002/(sici)1097-4695(199611)31:3 · Cited by 11 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Analytical techniques for multiplex analysis of protein biomarkers
van Gool, A. V., Corrales, F., Čolović, M., Krstić, D., Oliver-Martos, B., Martínez-Cáceres, E., Jakaša, I., Gajski, G., Brun, V., Kyriacou, K., et al. (2020) · Espert Review of Proteomics · doi:10.1080/14789450.2020.1763174 · Cited by 94 · see citing works
Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry
Buczak, K., Kirkpatrick, J. M., Truckenmueller, F., Santinha, D., Ferreira, L., Roessler, S., Singer, S., Beck, M., & Ori, A. (2020) · Nature Protocols · doi:10.1038/s41596-020-0356-y · Cited by 78 · see citing works
Herradón, G., Ramos, P., & Gramage, E. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.00377 · Cited by 72 · see citing works
A comprehensive systematic review of CSF proteins and peptides that define Alzheimer’s disease
Pedrero-Prieto, C. M., García‐Carpintero, S., Frontiñán-Rubio, J., Llanos-González, E., García, C. A., Alcain, F. J., Lindberg, I., Durán‐Prado, M., Peinado, J. R., & Rabanal‐Ruiz, Y. (2020) · Clinical Proteomics · doi:10.1186/s12014-020-09276-9 · Cited by 61 · see citing works
Proteomic analysis of protein homeostasis and aggregation
Laskowska, E., Kuczyńska‐Wiśnik, D., & Lipińska, B. (2018) · Journal of Proteomics · doi:10.1016/j.jprot.2018.12.003 · Cited by 49 · see citing works
Arc Regulates Transcription of Genes for Plasticity, Excitability and Alzheimer’s Disease
Leung, H.-W., Foo, G., & VanDongen, A. M. J. (2022) · Biomedicines · doi:10.3390/biomedicines10081946 · Cited by 39 · see citing works
Clinical Peptidomics: Advances in Instrumentation, Analyses, and Applications
Li, L., Wu, J., Lyon, C. J., Jiang, L., & Hu, Y. (2023) · BME Frontiers · doi:10.34133/bmef.0019 · Cited by 35 · see citing works
Progress and pitfalls of using isobaric mass tags for proteome profiling
Dayon, L., & Affolter, M. (2020) · Expert Review of Proteomics · doi:10.1080/14789450.2020.1731309 · Cited by 34 · see citing works
Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation
Linnerbauer, M., Lößlein, L., Farrenkopf, D., Vandrey, O., Tsaktanis, T., Naumann, U., & Rothhammer, V. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2021.800128 · Cited by 33 · see citing works
Feng, L., Huo, Z., Xiong, J., & Li, H. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c02381 · Cited by 30 · see citing works
Latosińska, A., Frantzi, M., & Siwy, J. (2023) · Mass Spectrometry Reviews · doi:10.1002/mas.21854 · Cited by 19 · see citing works
Cyclin-dependent kinase 5 mediates pleiotrophin-induced endothelial cell migration
Lampropoulou, E., Logoviti, I., Κουτσιούμπα, Μ., Hatziapostolou, M., Polytarchou, C., Skandalis, S. S., Hellman, U., Fousteris, M. A., Nikolaropoulos, S., Choleva, E., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-24326-x · Cited by 19 · see citing works
Song, Y., Li, H., Li, Y., Xu, H., Nazir, F. H., Jiang, W., Zheng, L., & Tang, C. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.11.013 · Cited by 14 · see citing works
An Adaptive Pipeline To Maximize Isobaric Tagging Data in Large-Scale MS-Based Proteomics
Corthésy, J., Theofilatos, K., Mavroudi, S. P., Macron, C., Cominetti, O., Remlawi, M., Ferraro, F., Galindo, A. N., Kussmann, M., LIKOTHANASSIS, S. D., et al. (2018) · Journal of Proteome Research · doi:10.1021/acs.jproteome.8b00110 · Cited by 14 · see citing works
The roles of pleiotrophin in brain injuries: a narrative review of the literature
Lei, Y., Zhou, R., Mao, Q., Qiu, X., & Mu, D. (2025) · Annals of Medicine · doi:10.1080/07853890.2025.2452353 · Cited by 13 · see citing works
Rodríguez-Zapata, M., López-Rodríguez, R., Ramos-Álvarez, M. D. P., Herradón, G., Pérez-García, C., & Gramage, E. (2024) · Toxicology · doi:10.1016/j.tox.2024.153947 · Cited by 11 · see citing works
Pedrero-Prieto, C. M., Frontiñán-Rubio, J., Alcain, F. J., Durán‐Prado, M., Peinado, J. R., & Rabanal‐Ruiz, Y. (2021) · Diagnostics · doi:10.3390/diagnostics11091655 · Cited by 12 · see citing works
Loss of <i>Inpp5d</i> has disease‐relevant and sex‐specific effects on glial transcriptomes
Dabin, L. C., Kersey, H., Kim, B., Acri, D. J., Sharify, D., Lee‐Gosselin, A., Lasagna‐Reeves, C. A., Oblak, A. L., Lamb, B. T., & Kim, J. (2024) · Alzheimer's & Dementia · doi:10.1002/alz.13901 · Cited by 10 · see citing works
Zhou, Y., Li, H., Liu, X., Chi, X., Gu, Z., Cui, B., Bergquist, J., Wang, B., Tian, G., Yang, C., et al. (2023) · Journal of Proteome Research · doi:10.1021/acs.jproteome.3c00269 · Cited by 10 · see citing works
Pilot proteomic analysis of cerebrospinal fluid in Alzheimer's disease
McKetney, J., Panyard, D. J., Johnson, S. C., Carlsson, C. M., Engelman, C. D., & Coon, J. J. (2021) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.202000072 · Cited by 9 · see citing works
Drake, S., Zaman, A., Simas, T., & Fournier, A. E. (2023) · WIREs Mechanisms of Disease · doi:10.1002/wsbm.1594 · Cited by 11 · see citing works
Farrell, C., Buhidma, Y., Mumford, P., Heywood, W. E., Hällqvist, J., Flores-Aguilar, L., Andrews, E. J., Rahimzadah, N., Taso, O. S., Doran, E., et al. (2025) · Acta Neuropathologica · doi:10.1007/s00401-025-02889-0 · Cited by 9 · see citing works
İlgün, A., & Çakır, T. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-04965-8 · Cited by 11 · see citing works
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202 · Cited by 9 · see citing works
Reyes-Mata, M. P., Rojas-Mayorquín, A. E., Carrera-Quintanar, L., González-Castillo, C., Mireles-Ramírez, M. A., Guerrero-García, J. D. J., & Ortuño‐Sahagún, D. (2021) · Archives of Medical Research · doi:10.1016/j.arcmed.2021.06.005 · Cited by 9 · see citing works
Liu, T., Guo, W., Gong, M., Zhu, L., Cao, T., Huang, Y., Yang, Y., Chen, J., Yang, C., Zhang, S., et al. (2025) · Experimental Brain Research · doi:10.1007/s00221-025-07134-4 · Cited by 6 · see citing works
Positive association between PTN polymorphisms and schizophrenia in Northeast Chinese Han population
Lv, Y., Sun, Y., Dai, D., Luan, Z., Lu, H., Li, C.-J., & Luo, Y. (2020) · Psychiatric Genetics · doi:10.1097/ypg.0000000000000262 · Cited by 6 · see citing works
Sensi, S. L., Russo, M., & Tiraboschi, P. (2023) · Handbook of clinical neurology · doi:10.1016/b978-0-323-85538-9.00015-8 · Cited by 5 · see citing works
Proteomics-based aging clocks in midlife or late-life and their associated risk of dementia
Sedaghat, S., Park, S., Walker, R. F., Wang, S., Liu, J., Hughes, T. M., Sabayan, B., Tang, W., Coresh, J., Pankow, J. S., et al. (2025) · Communications Medicine · doi:10.1038/s43856-025-01096-y · Cited by 6 · see citing works
Zhang, W., Xu, Z., Zhang, X., Yan, Y., Deng, C., & Sun, N. (2024) · Talanta · doi:10.1016/j.talanta.2024.125948 · Cited by 4 · see citing works
Symptomatic vs. Disease-Modifying Therapies
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.011 · Cited by 2 · see citing works
Liu, J., Fan, J., Liu, H., Chen, M., Fu, X., Zhao, Y., Cheng, M., Guo, Y., Li, Y., Liu, J., et al. (2025) · Journal of Translational Medicine · doi:10.1186/s12967-025-07172-0 · Cited by 1 · see citing works
Disease Subtypes: The Promise and the Fallacy
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.006 · Cited by 1 · see citing works
Detecting single amino acids and small peptides by combining isobaric tags and peptidomics
Burger, B., Lereim, R. R., Berven, F. S., & Barsnes, H. (2019) · European Journal of Mass Spectrometry · doi:10.1177/1469066719857006 · Cited by 1 · see citing works
Hansson, K. (2018) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Self-citationThe Hypothesis That Refuses to Die
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.012
Диалог культур в европейском образовательном пространстве
Николаевич, К. К. (2010) · Знание. Понимание. Умение
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.020
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.013
Disease “Redefinition”: A Tough Pill to Swallow
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.005
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.008
The Shaky Six and the “Second Reality”
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.003
Predictions for the 2020s and Beyond
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.017
The Scientific and Lay Narratives
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.014
Note Added at Press Time – Reviving LOF
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.019
Espay, A. J., & Stecher, B. (2020) · doi:10.1017/9781108888202.001
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.015
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.018
The Moonshot: Population-Based Studies of Aging
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.016
Espay, A. J., & Stecher, B. (2020) · doi:10.1017/9781108888202.002
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.010
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.004
Biomarkers: The Promise and the Fallacy
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.009
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.007
Espay, A. J., & Stecher, B. (2020) · Cambridge University Press eBooks · doi:10.1017/9781108888202.021
Serum pleiotrophin levels in patients with multiple sclerosisPreprint
Civelek, E., Yıldız, Ö. K., Kablan, D., & Doğan, H. O. (2024) · Research Square · doi:10.21203/rs.3.rs-4490063/v1
Genetic Modifiers of ALS: The Impact of Chromogranin B P413L in a Bulgarian ALS Cohort
Tourtourikov, I., Todorov, T., Angelov, T., Chamova, T., Tournev, I., Mitev, V., & Тодорова, А. (2024) · Genes · doi:10.3390/genes15091197
Exploratory Blood Biomarker Patterns in a Mixed Dementia CohortPreprint
Gezegen, H., Alaylıoğlu, M., Şahin, E., Swann, O., Veleva, E., Güven, G., Yaman, Ü., Salih, D. A. M., Bılgıç, B., Hanağası, H. A., et al. (2024) · medRxiv · doi:10.1101/2024.11.07.24316927
Self-citationHuang, L., Liu, B., Huang, Q., Zhou, G., Deng, W., & Li, X. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.116046
Rathbun, A. M., Chen, C., Tian, Q., Tanaka, T., Ferrucci, L., & Shardell, M. D. (2026) · GeroScience · doi:10.1007/s11357-026-02356-y
Fontán‐Baselga, T., Cañeque‐Rufo, H., Rivera-Illades, E., Gramage, E., Zapico, J. M., de Pascual‐Teresa, B., Ramos-Álvarez, M. D. P., Vicente‐Rodríguez, M., & Herradón, G. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1839516
- Authors
- Skillbäck, T., Mattsson, N., Hansson, K., Mirgorodskaya, E., Dahlén, R., van der Flier, W., Scheltens, P., Duits, F., Hansson, O., Teunissen, C., Blennow, K., Zetterberg, H., & Gobom, J.
- Affiliations
- Sahlgrenska University Hospital (SE)
- Lund University (SE)
- University of Gothenburg (SE)
- Amsterdam Neuroscience (NL)
- Corresponding author
- Gobom, Johan
- ORCID
- Venue
- Scientific Reports
- Published
- 2017-10-17
- Volume
- 7
- Issue
- 1
- Pages
- 13333-13333
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5645330
- OpenAlex
- W2763859909
- Cited by
- 61
- References
- 58
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 7 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 28 · 2019: 2 · 2018: 4
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion, cc-by)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Torsten Söderbergs Stiftelse
- Demensförbundet
- Lunds Universitet
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet
- Sahlgrenska Universitetssjukhuset
- Magnus Bergvalls Stiftelse
- Alzheimerfonden
- Emil och Wera Cornells Stiftelse
- Parkinsonfonden
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- Alzheimer's disease, biomarker discovery, pleiotrophin, cerebrospinal fluid, spectral clustering, isobaric labeling, mild cognitive impairment, Parkinson's disease, progressive supranuclear palsy, peptide fragments
- MeSH terms
- Alzheimer Disease, Carrier Proteins, Female, Humans, Male, Peptides, ROC Curve, Reproducibility of Results, Biomarkers, Cytokines, Proteome, Proteomics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
