Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues
Open accessAbstract
L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities in vitro , and possible cytoprotective capabilities in vivo . We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SO 3 H), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases.
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A number of high-dose studies indicate ERG is safe for mammalian consumption at levels far in excess of those likely in foodstuffs, and it has been declared safe by EFSA.
“Notwithstanding the highly variable intake between individuals(358), however, a number of high-dose studies have indicated that ERG is safe for mammalian consumption at levels far in excess of those likely to be encountered in foodstuffs(125,131,359,360), and it has been declared safe by relevant committees such as those of the European Food Standards Agency(361,362).”
From The biology of ergothioneine, an antioxidant nutraceutical (2020) · cites this paper as [131]
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Gene Knockout and Metabolome Analysis of Carnitine/Organic Cation Transporter OCTN1
Kato, Y., Kubo, Y., Iwata, D., Kato, S., Sudo, T., Sugiura, T., Kagaya, T., Wakayama, T., Hirayama, A., Sugimoto, M., et al. (2010) · Pharmaceutical Research · doi:10.1007/s11095-010-0076-z · Cited by 206 · see citing works
Nakamichi, N., Nakayama, K., Ishimoto, T., Masuo, Y., Wakayama, T., Sekiguchi, H., Sutoh, K., Usumi, K., Iseki, S., & Kato, Y. (2016) · Brain and Behavior · doi:10.1002/brb3.477 · Cited by 107 · see citing works
COLOGNATO, R., LAURENZA, I., FONTANA, I., COPPEDE, F., SICILIANO, G., COECKE, S., ARUOMA, O., BENZI, L., & MIGLIORE, L. (2006) · Clinical Nutrition · doi:10.1016/j.clnu.2005.10.005 · Cited by 81 · see citing works
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The biology of ergothioneine, an antioxidant nutraceutical
Borodina, I., Kenny, L. C., McCarthy, C. M., Paramasivan, K., Pretorius, E., Roberts, T. J., van der Hoek, S. A., & Kell, D. B. (2020) · Nutrition Research Reviews · doi:10.1017/s0954422419000301 · Cited by 296 · see citing works
“to be encountered in foodstuffs(125,131,359,360), and it has been”
BackgroundFeng, L., Cheah, I. K.-M., Ng, M. M.-X., Li, J., Chan, S. M., Lim, S. L., Mahendran, R., Kua, E.-H., & Halliwell, B. (2019) · Journal of Alzheimer's Disease · doi:10.3233/jad-180959 · Cited by 112 · see citing works
Self-citationReflections of an aging free radical
Halliwell, B. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.10.010 · Cited by 75 · see citing works
Self-citationSpecificity of the ergothioneine transporter natively expressed in HeLa cells
Tucker, R. A. J., Cheah, I. K., & Halliwell, B. (2019) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2019.02.122 · Cited by 38 · see citing works
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- Authors
- Tang, R. M. Y., Cheah, I. K.-M., Yew, T. S. K., & Halliwell, B.
- Affiliations
- National University of Singapore (SG)
- Corresponding author
- Halliwell, Barry
- Venue
- Scientific Reports
- Published
- 2018-01-25
- Volume
- 8
- Issue
- 1
- Pages
- 1601-1601
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5785509
- OpenAlex
- W2800336108
- Cited by
- 163
- References
- 3
- Citations by year
- 2026: 28 · 2025: 27 · 2024: 19 · 2023: 19 · 2022: 23 · 2021: 25 · 2020: 10 · 2019: 10 · 2018: 2
- FWCI
- 5.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Medical Research Council
- National Medical Research Council (NMRC/1264/2010/082/12, NMRC/1264/2010)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Keywords
- ergothioneine, tissue distribution, oral administration, liver, brain, antioxidant amino acids, oxidative damage, dietary amino acids
- MeSH terms
- Administration, Oral, Animals, Animal Structures, Antioxidants, Chromatography, Liquid, Ergothioneine, Male, Mice, Inbred C57BL, Tandem Mass Spectrometry
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