The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Imaging of human cells exposed to an antifungal antibiotic amphotericin B reveals the mechanisms associated with the drug toxicity and cell defence
Open accessAbstract
Amphotericin B is an antibiotic used in pharmacotherapy of life-threatening mycotic infections. Unfortunately, the applicability of this antibiotic is associated with highly toxic side effects. In order to understand molecular mechanisms underlying toxicity of amphotericin B to patients, two cell lines, human normal colon epithelial cells (CCD 841 CoTr) and human colon adenocarcinoma cells (HT-29) were cultured in the presence of the drug and imaged with the application of fluorescence lifetime imaging microscopy and Raman scattering microscopy. The results of the cell viability assays confirm high toxicity of amphotericin B towards human cells. The images recorded demonstrate effective binding of amphotericin B to biomembranes. Analysis of the images reveals the operation of a defence mechanism based upon the elimination of molecules of the drug from living cells via formation of small amphotericin B-containing lipid vesicles. The fact that exosomes formed are devoid of cholesterol, as concluded on the basis of the results of Raman analysis, suggests that sequestration of sterols from the lipid phase of biomembranes is not a sole mechanism responsible for the toxic side effects of amphotericin B. Alternatively, the results imply that molecules of the drug present directly within the hydrophobic membrane core disturb the lipid membrane structure and affect their biological functions.
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Amphotericin B has serious toxicity limitations on human host cells.
“Despite these therapeutic advantages, AmB has serious toxicity limitations on the human host cells.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [108]
Both human and fungal cell biomembranes are primary targets of amphotericin B, impairing physiological processes in membranes, particularly adenocarcinoma cells.
“This is because both human and fungal cells’ biomembranes are the primary targets of the AmB. Thus, impairing the physiological processes that take place in the membranes, particularly adenocarcinoma cells [108].”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [108]
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Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival
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Metadata
- Authors
- Grela, E., Piet, M., Luchowski, R., Grudzinski, W., Paduch, R., & Gruszecki, W. I.
- Affiliations
- Maria Curie-Skłodowska University (PL)
- Medical University of Lublin (PL)
- Venue
- Scientific Reports
- Published
- 2018-09-14
- Volume
- 8
- Issue
- 1
- Pages
- 14067-14067
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6138690
- OpenAlex
- W2890319039
- Cited by
- 38
- References
- 21
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 9 · 2020: 7 · 2019: 7
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Narodowe Centrum Nauki (2015/19/B/NZ7/02159)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- amphotericin B, drug toxicity, antifungal antibiotics, cell membrane binding, exosomes, lipid vesicles, cholesterol sequestration, membrane sterols, membrane structure, colonic epithelial cells, colon adenocarcinoma cells, fluorescence lifetime imaging
- MeSH terms
- Amphotericin B, Anti-Bacterial Agents, Antifungal Agents, Cell Survival, Humans, Microscopy, Fluorescence, Spectrum Analysis, Raman, HT29 Cells
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