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Cited by 16Cardiac electrophysiology and arrhythmiasAtrial Fibrillation Management and OutcomesCardiac Arrhythmias and Treatments

An implantable system for long-term assessment of atrial fibrillation substrate in unanesthetized rats exposed to underlying pathological conditions

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Klapper-Goldstein, Hadar, Murninkas, Michael, Gillis, Roni, Mulla, Wesam, Levanon, Eran, Elyagon, Sigal, Schuster, Ronen, Danan, Dor +2 more·2020·Scientific Reports
DOI 10.1038/s41598-020-57528-3PMC PMC6969190
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Abstract

Atrial fibrillation (AF) is a progressive arrhythmia with underlying mechanisms that are not fully elucidated, partially due to lack of reliable and affordable animal models. Here, we introduce a system for long-term assessment of AF susceptibility (substrate) in ambulatory rats implanted with miniature electrodes on the atrium. Rats were subjected to excessive aldosterone (Aldo) or solvent only (Sham). An additional group was exposed to myocardial infarction (MI). AF substrate was tested two- and four-weeks post implantation and was also compared with implanted rats early post-implantation (Base). Aldo and MI increased the AF substrate and atrial fibrosis. In the MI group only, AF duration was correlated with the level of atrial fibrosis and was inversely correlated with systolic function. Unexpectedly, Shams also developed progressive AF substrate relative to Base individuals. Further studies indicated that serum inflammatory markers (IL-6, TNF-alpha) were not elevated in the shams. In addition, we excluded anxiety\depression due to social-isolation as an AF promoting factor. Finally, enhanced biocompatibility of the atrial electrode did not inhibit the gradual development of AF substrate over a testing period of up to 8 weeks. Overall, we successfully validated the first system for long-term AF substrate testing in ambulatory rats.

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Cited by

Sorted by citations

Authors
Klapper-Goldstein, H., Murninkas, M., Gillis, R., Mulla, W., Levanon, E., Elyagon, S., Schuster, R., Danan, D., Cohen, H., & Etzion, Y.
Affiliations
Ben-Gurion University of the Negev (IL)
Corresponding author
Etzion, Yoram
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-01-17
Volume
10
Issue
1
Pages
553-553
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-020-57528-3
PMC
PMC6969190
OpenAlex
W2998874206
Cited by
16
References
2
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 2
FWCI
2.01
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Israel Science Foundation (1174/14, 1233/19)
  • Israel Heart Society
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Atrial Fibrillation Management and Outcomes
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Arrhythmias and Treatments
Keywords
atrial fibrillation, atrial fibrillation substrate, aldosterone, myocardial infarction, atrial fibrosis, atrial systolic function, inflammatory markers, social isolation
MeSH terms
Aldosterone, Animals, Atrial Fibrillation, Disease Models, Animal, Electrodes, Implanted, Fibrosis, Male, Microelectrodes, Myocardial Infarction, Tumor Necrosis Factor-alpha, Interleukin-6, Rats, Sprague-Dawley, Rats

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