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Cited by 20Nanoparticles: synthesis and applicationsAntimicrobial agents and applicationsNanocomposite Films for Food Packaging

Inactivation of SARS-CoV-2 by a chitosan/α-Ag2WO4 composite generated by femtosecond laser irradiation

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Pereira, Paula Fabiana Santos, de Paula e Silva, Ana Carolina Alves, da Silva Pimentel, Bruna Natália Alves, Pinatti, Ivo Mateus, Simões, Alexandre Zirpoli, Vergani, Carlos Eduardo, Barreto-Vieira, Débora Ferreira, da Silva, Marcos Alexandre Nunes +7 more·2022·Scientific Reports
DOI 10.1038/s41598-022-11902-5PMC PMC9114143
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Abstract

In the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag 2 WO 4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag 2 WO 4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARS-CoV-2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α-Ag 2 WO 4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag 2 WO 4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 µg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag 2 WO 4 (CS6.6/α-Ag 2 WO 4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production.

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Cited by

Sorted by citations

Authors
Pereira, P. F. S., de Paula e Silva, A. C. A., da Silva Pimentel, B. N. A., Pinatti, I. M., Simões, A. Z., Vergani, C. E., Barreto-Vieira, D. F., da Silva, M. A. N., Miranda, M. D., Monteiro, M. E. S., Tucci, A., Doñate-Buendía, C., Mínguez-Vega, G., Andrés, J., & Longo, E.
Affiliations
  • Universitat Jaume I (ES)
  • Universidade Estadual Paulista (Unesp) (BR)
  • Instituto Oswaldo Cruz (BR)
  • Universidade Federal de São Carlos (BR)
Corresponding author
Longo, Elson
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2022-05-17
Volume
12
Issue
1
Pages
8118-8118
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-022-11902-5
PMC
PMC9114143
OpenAlex
W4280494846
Cited by
20
References
1
Citations by year
2026: 2 · 2025: 6 · 2024: 8 · 2023: 3 · 2022: 1
FWCI
1.27
Type
journal article
Language
en
Open access
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  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Arca - Repositório Institucional da Fiocruz (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Repositori UJI (Universitat Jaume I) (submittedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Funders
  • Ministerio de Ciencia, Innovación y Universidades (PGC2018-094417-B-I00, 2013/07296-2, UJI-B2019-30, PID2019)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (13/07296-2, 2013/072962, #2013/07296-, 2019/, AICO2020, 2014/, PGC2018-094417-B-I00, 2014/14171-4, 2017/13008-0, 2019/03722-3, UJI-B2019-30, 2019/25944-8)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (UJI-B2019-30, 2013/07296–2, PGC2018-094417-B-I00)
  • Generalitat Valenciana (UJI-B2019-37, 2013/07296-2, PID2019-110927RB-I00, AICO2020, UJI-B2019-30, PROMETEO/2020/029)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (2013/07296-2, UJI-B2019-30)
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
  • Universitat Jaume I (PROMETEO/2020/029, UJI-B2019-37, UJI- B2019-30, Project UJI-B2019-30, AICO2020, 2013/07296-2, PID2019-110927RB-I00)
  • Ministerio de Ciencia e Innovación (B-I00, PROMETEO/2020/029, PID2019, PID2019-110927RB-I00, PGC2018094417-B-I00, UJI-B2019-37)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
  • Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
Keywords
SARS-CoV-2 inactivation, silver tungstate, antimicrobial activity, Escherichia coli, methicillin-susceptible Staphylococcus aureus, Candida albicans, cytotoxicity, reactive oxygen species, COVID-19 prevention, antiviral materials
MeSH terms
COVID-19, SARS-CoV-2, Anti-Bacterial Agents, Candida albicans, Escherichia coli, Escherichia coli Infections, Humans, Lasers, Microbial Sensitivity Tests, Staphylococcal Infections, Staphylococcus aureus, Chitosan, Pandemics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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