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Cited by 20Plant tissue culture and regenerationEpigenetics and DNA MethylationPlant Molecular Biology Research

Epigenomic insight of lingonberry and health-promoting traits during micropropagation

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Sikdar, Arindam, Sharma, Umanath, Barua, Rajesh, Igamberdiev, Abir U., Debnath, Samir C.·2022·Scientific Reports
DOI 10.1038/s41598-022-16530-7PMC PMC9304418

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Abstract

Epigenetic variation plays a role in developmental gene regulation and responses to the environment. An efficient interaction of zeatin-induced cytosine methylation and secondary compounds has been displayed for the first time in tissue-culture shoots/plants of lingonberry ( Vaccinium vitis-idaea L.) cultivar Erntedank in vitro (NC1, in a liquid medium; NC2, on a semi-solid medium), ex vitro (NC3, node culture-derived plants; LC1, leaf culture-derived plants) and its cutting-propagated (ED) plants. Through methylation-sensitive amplification polymorphism (MSAP) assay, we observed highest methylated sites in leaf regenerants (LC1) from all primer combinations (108 bands), along with the highest secondary metabolites. The four types of tissue culture-derived shoots/plants (NC1, NC2, NC3, LC1) showed higher methylation bands than cutting propagated donor plants (ED) that exhibited 79 bands of methylation, which is comparatively low. Our study showed more methylation in micropropagated shoots/plants than those derived from ED plants. On the contrary, we observed higher secondary metabolites in ED plants but comparatively less in micropropagated shoots (NC1, NC2) and plants (NC3, LC1).

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  • In Vaccinium vitis-idaea, the highest methylation was observed in leaf regenerants (LC1), which also showed the highest levels of secondary metabolites.

    “In Vaccinium vitis-idaea, through MSAP analysis, the highest methylation was observed in leaf regenerants (LC1), which also showed the highest levels of secondary metabolites.”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [93]

    The Abstract reports that MSAP found the highest methylation in leaf regenerants (LC1) and describes them as having the highest secondary metabolites. The Results section, “Portraying the partnerships of cytosine methylation and secondary metabolites,” qualifies that description: LC1 had relatively high phenolic content, but low flavonoid content, the lowest proanthocyanidin content, and antioxidant activity tied with other groups. Thus the claim is supported for methylation but overgeneralizes the metabolite findings. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Micropropagated Vaccinium vitis-idaea plants (NC1, NC2, NC3, LC1) exhibited more methylation than cutting-propagated (ED) plants, but ED plants had higher levels of secondary metabolites, indicating a trade-off between methylation and metabolite production.

    “Micropropagated plants (NC1, NC2, NC3, LC1) exhibited more methylation than cutting-propagated (ED) plants, but ED plants had higher levels of secondary metabolites, indicating a trade-off between methylation and metabolite production [93].”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [93]

  • In Vaccinium vitis-idaea, more methylation events are observed in in vitro-derived plants than in those derived from cuttings.

    “More methylation events are observed in vitro-derived from cuttings of plants plants than in those derived”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [93]

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Authors
Sikdar, A., Sharma, U., Barua, R., Igamberdiev, A. U., & Debnath, S. C.
Affiliations
  • Memorial University of Newfoundland (CA)
  • Agriculture and Agri-Food Canada (CA)
Corresponding author
Debnath, Samir C.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2022-07-21
Volume
12
Issue
1
Pages
12487-12487
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-022-16530-7
PMC
PMC9304418
OpenAlex
W4286383322
Cited by
20
References
60
Citations by year
2026: 6 · 2025: 9 · 2024: 2 · 2023: 2 · 2022: 1
FWCI
1.14
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Memorial University of Newfoundland
  • Agriculture and Agri-Food Canada
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
lingonberry, epigenetics, DNA methylation, micropropagation, secondary metabolites, zeatin, methylation-sensitive amplification polymorphism, cutting propagation, tissue culture-derived plants
MeSH terms
Polymorphism, Genetic, Plant Leaves, DNA Methylation, Vaccinium vitis-idaea, Epigenomics

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