Epigenetics in plant tissue culture
Open accessAbstract
Plants produced vegetatively in tissue culture may differ from the plants from which they have been derived. Two major classes of off-types occur: genetic ones and epigenetic ones. This review is about epigenetic aberrations. We discuss recent studies that have uncovered epigenetic modifications at the molecular level, viz. , changes in DNA methylation and alterations of histone methylation or acetylation. Various studies have been carried out with animals, and with plant cells or tissues that have grown in tissue culture but only little work has been done with shoots generated by axillary branching. We present various molecular methods that are being used to measure epigenetic variation. In micropropagated plants mostly differences in DNA methylation have been examined. Epigenetic changes are thought to underlie various well-known tissue-culture phenomena including rejuvenation, habituation, and morphological changes such as flower abnormalities, bushiness, and tumorous outgrowths in, among others, oil palm, gerbera, Zantedeschia and rhododendron.
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In the Arabidopsis genome, histone modifications such as H2BK143 ubiquitination, H3K4/H3K36/H3 methylation, and H3/H4 lysine acetylation are associated with gene activation, while H3K9/H3K27/H4K20 methylation and H4R3 dimethylation are associated with gene inactivation.
“For example, it is well known that modifications such as the ubiquitination of H2BK143, mono-, di-, and trimethylation of H3K4, H3K36, and H3 and H4 lysine acetylation are associated, in Arabidopsis genome, with gene activation, while the mono-, di- and trimethylation of H3H9, H3K27, and H4K20, and the dimethylation of H4R3, are associated with gene inactivation [90,91,92,93]).”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [93]
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- Authors
- Smulders, M. J. M., & de Klerk, G. J.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Smulders, M. J. M.
- ORCID
- Smulders, M. J. M.
- Venue
- Plant Growth Regulation
- Published
- 2010-10-08
- Volume
- 63
- Issue
- 2
- Pages
- 137-146
- ISSN
- 0167-6903 (ISSN-L)
- 1573-5087
- OpenAlex
- W1997233908
- Cited by
- 249
- References
- 4
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 20 · 2023: 16 · 2022: 17 · 2021: 27 · 2020: 19 · 2019: 21 · 2018: 16 · 2017: 8 · 2016: 16 · 2015: 19 · 2014: 16 · 2013: 14 · 2012: 12
- FWCI
- 4.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Plant Growth Regulation (publishedVersion, cc-by-nc)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- epigenetics, plant tissue culture, off-types, DNA methylation, histone methylation, histone acetylation, micropropagation, rejuvenation, habituation, morphological abnormalities, oil palm, Gerbera, Zantedeschia, Rhododendron
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