The dynamics of telomere length in primary and metastatic colorectal cancer lesions
Open accessAbstract
Telomeric sequences, the structures comprised of hexanucleotide repeats and associated proteins, play a pivotal role in chromosome end protection and preservation of genomic stability. Herein we address telomere length (TL) dynamics in primary colorectal cancer (CRC) tumour tissues and corresponding liver metastases. TL was measured by multiplex monochrome real-time qPCR in paired samples of primary tumours and liver metastases along with non-cancerous reference tissues obtained from 51 patients diagnosed with metastatic CRC. Telomere shortening was observed in the majority of primary tumour tissues compared to non-cancerous mucosa (84.1%, p < 0.0001). Tumours located within the proximal colon had shorter TL than those in the rectum (p < 0.05). TL in liver metastases was not significantly different from that in primary tumours (p = 0.41). TL in metastatic tissue was shorter in the patients diagnosed with metachronous liver metastases than in those diagnosed with synchronous liver metastases (p = 0.03). The metastatic liver lesions size correlated with the TL in metastases (p < 0.05). Following the neoadjuvant treatment, the patients with rectal cancer had shortened telomeres in tumour tissue than prior to the therapy (p = 0.01). Patients with a TL ratio between tumour tissue and the adjacent non-cancerous mucosa of ≥ 0.387 were associated with increased overall survival (p = 0.01). This study provides insights into TL dynamics during progression of the disease. The results show TL differences in metastatic lesions and may help in clinical practice to predict the patient’s prognosis.
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Telomere length has emerged as a potential predictive biomarker for the clinical outcome of anti-EGFR monoclonal antibody therapy in patients with KRAS wild-type metastatic CRC.
“Furthermore, TL has emerged as a potential predictive biomarker for the clinical outcome of anti-EGFR monoclonal antibody therapy in patients with KRAS wild-type metastatic CRC.”
From Current and Future Trends of Colorectal Cancer Treatment: Exploring Advances in Immunotherapy (2024) · cites this paper as [24]
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Current and Future Trends of Colorectal Cancer Treatment: Exploring Advances in Immunotherapy
Nikolouzakis, T. K., Chrysos, E., Docea, A. O., Fragkiadaki, P., Souglakos, J., Tsiaoussis, J., & Tsatsakis, A. (2024) · Cancers · doi:10.3390/cancers16111995 · Cited by 37 · see citing works
- Authors
- Kroupa, M., Kubecek, O., Tomasova, K., Hanak, P., Krupova, M., Cervena, K., Siskova, A., Rosendorf, J., Hosek, P., Vodickova, L., Vodicka, P., Liska, V., John, S., Vymetalkova, V., & Petera, J.
- Affiliations
- Charles University (CZ)
- University Hospital Hradec Králové (CZ)
- Corresponding author
- Kroupa, Michal
- Venue
- Scientific Reports
- Published
- 2023-06-05
- Volume
- 13
- Issue
- 1
- Pages
- 9097-9097
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC10241861
- OpenAlex
- W4379471589
- Cited by
- 10
- References
- 53
- Citations by year
- 2026: 7 · 2025: 1 · 2024: 2
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- the Czech health research council of the Ministry of Health of the Czech Republic (NU22J-03-00028)
- Charles University (GA UK No. 120)
- the Czech Science Foundation (21-27902S)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Health Sciences › Medicine › Oncology › Colorectal Cancer Treatments and Studies
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Keywords
- telomere length, colorectal cancer, liver metastasis, telomere shortening, metachronous metastasis, synchronous metastases, neoadjuvant therapy, overall survival, prognosis, tumor location, metastasis size
- MeSH terms
- Colonic Neoplasms, Humans, Liver Neoplasms, Prognosis, Rectal Neoplasms, Colorectal Neoplasms, Telomere, Telomere Shortening
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