Accelerated Wound Healing Induced by Spinach Extract in Experimental Model Diabetic Rats with Streptozotocin
Open accessAbstract
Patients with diabetes often have difficult-to-heal wounds. Spinacia oleracea extract comprises anti-inflammatory and anti-oxidative compounds; this research, therefore, studied the impact of Spinacia oleracea extracts on ulcer regeneration. This study was conducted on 72 adult Wistar rats (200 [Formula: see text] 20 g). They were randomly divided into six groups of twelve. A: Diabetic group receiving normal saline. B: Non-diabetic group receiving normal saline. C: Diabetic group receiving spinach aqueous extract. D: Diabetic group receiving spinach alcoholic extract. E: preventive group that received aqueous extract for 2 months. F: preventive group that received alcoholic extract for 2 months. Ulcer regeneration, vascular endothelium growth factor, blood sugar, and weight changes were measured on days 3, 7, 14, 21, and 30. Macroscopic investigation of the wounds non-diabetic control group, diabetic group, as well as spinach aqueous and alcoholic extract groups, were compared and there were significant changes (P < 0.05). Pathologic examination in the spinach aqueous and alcoholic extract groups, and nondiabetic group than in the diabetic group revealed significant advances (P < 0.05). On the third and seventh days, Vascular endothelium growth factor detected significant differences between groups (P < 0.05). Results indicate that, in regenerating diabetic ulcers, Spinacia oleracea may be effective. It influences the ulcer structure and speed.
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Spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage.
“These results show that spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage [156,162,164,165,166,167,168,169,170].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [167]
The Abstract (page unavailable) describes spinach’s antioxidant compounds as protecting cells from oxidative damage, and the Discussion (page unavailable) reports faster wound healing with spinach extract in diabetic rats. The paper does not report a lipid-lowering effect; its Discussion mentions a hormone associated with spinach thylakoids but does not establish appetite regulation by spinach. The cited reference titles alone do not fill those gaps. (LLM, conf=0.86)
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- Authors
- Rahati, S., Kamalinezhad, M., Ebrahimi, A., Eshraghian, M., & Pishva, H.
- Affiliations
- Zahedan University of Medical Sciences (IR)
- Shahid Beheshti University (IR)
- Tehran University of Medical Sciences (IR)
- Corresponding author
- Pishva, Hamideh
- Venue
- Scientific Reports
- Published
- 2023-09-11
- Volume
- 13
- Issue
- 1
- Pages
- 14933-14933
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC10495437
- OpenAlex
- W4386607241
- Cited by
- 9
- References
- 51
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Tehran University of Medical Sciences and Health Services (21554)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Keywords
- spinach, spinach extract, diabetic wound healing, diabetic ulcers, streptozotocin-induced diabetes, vascular endothelial growth factor, aqueous extract, alcoholic extract, antioxidant activity, anti-inflammatory activity, wound regeneration
- MeSH terms
- Saline Solution, Animals, Diabetes Mellitus, Experimental, Streptozocin, Ulcer, Wound Healing, Rats, Wistar, Spinacia oleracea, Intercellular Signaling Peptides and Proteins, Rats
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