Evaluation of Chlorella vulgaris biosorption capacity for phosphate and nitrate removal from wastewater
Open accessAbstract
High wastewater production rates during the past few decades are mostly attributable to anthropogenic activities. The main components leading to the nutrient enrichment of natural water bodies are such as nitrogen, phosphorus, and other minerals. The main focus of this research was to assess the ability of using Chlorella vulgaris algae, a potent and environmentally benign material, to eliminate phosphate and nitrate ions from wastewater. FTIR results showed that the biologically active molecules that facilitate the binding of phosphate and nitrate ions unto the C. vulgaris are C=C and N–H amid. The ideal equilibrium time for adsorption was 24 h with an optimum pH of 7 and the mass ratio of algae and different anions concentration was 80%. Freundlich isotherm model was the best-fitted isotherm. Moreover, the results of the experiment fit more closely with the pseudo-second-order kinetic model than other models. Elovich kinetic model data for both ions showed that the adsorption rate was much higher than the desorption rate. The growing popularity of biosorbents in treating wastewater has led to an improvement in their affordability and availability, and C. vulgaris may now represent an environmentally friendly choice from an environmental, and economic standpoint.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Chlorella vulgaris and Scenedesmus obliquus have been successfully cultivated in wastewater and have aided in removal of pollutants like nitrogen and phosphorus.
“For instance, Chlorella vulgaris and Scenedesmus obliquus have been successfully cultivated in wastewater and have aided in the removal of pollutants like nitrogen and phosphorus [77,78].”
From A Review of Algae-Based Carbon Capture, Utilization, and Storage (Algae-Based CCUS) (2024) · cites this paper as [77]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A.O, D. (2012) · IOSR Journal of Applied Chemistry · doi:10.9790/5736-0313845 · Cited by 1,934 · see citing works
Nutrient recovery from wastewater streams by microalgae: Status and prospects
Cai, T., Park, S. Y., & Li, Y. (2012) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2012.11.030 · Cited by 1,585 · see citing works
Integrating micro-algae into wastewater treatment: a review
Mohsenpour, S. F., Hennige, S., Willoughby, N., Adeloye, A., & Gutierrez, T. (2021) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.142168 · Cited by 828 · see citing works
Microalgae-based wastewater treatment: Mechanisms, challenges, recent advances, and future prospects
Abdelfattah, A., Ali, S. S., Ramadan, H., El-Aswar, E. I., Eltawab, R., Ho, S.-H., Elsamahy, T., Li, S., El-Sheekh, M. M., Schagerl, M., et al. (2023) · Environmental Science and Ecotechnology · doi:10.1016/j.ese.2022.100205 · Cited by 812 · cited 1× · see citing works
“Due to their fast rate of development and excellent adaptation for different wastewater streams, strains within the genus C. vulgaris have been widely employed and discovered to be ideal for growth in wastewater 17 .”
BackgroundBanerjee, S., & Chattopadhyaya, M. C. (2017) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2013.06.005 · Cited by 791 · see citing works
Monolayer and multilayer adsorption isotherm models for sorption from aqueous media
Saadi, R., Saadi, Z., Fazaeli, R., & Fard, N. E. (2015) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-015-0053-7 · Cited by 712 · see citing works
Kinetic and thermodynamics of chromium ions adsorption onto low-cost dolomite adsorbent
Albadarin, A. B., Mangwandi, C., Al-Muhtaseb, A. H., Walker, G., Allen, S. J., & Ahmad, M. N. (2011) · Chemical Engineering Journal · doi:10.1016/j.cej.2011.10.080 · Cited by 538 · see citing works
Nitrogen and phosphate removal from wastewater with a mixed microalgae and bacteria culture
Delgadillo-Mirquez, L., Lopes, F., Taidi, B., & Pareau, D. (2016) · Biotechnology Reports · doi:10.1016/j.btre.2016.04.003 · Cited by 488 · see citing works
López–Luna, J., Ramírez-Montes Loida, E., Vargas, S. M., Martı́nez, A. I., Mijangos-Ricárdez, Ó. F., Gónzalez‐Chávez, M. D. C. A., Carrillo‐González, R., Solís-Domínguez, F. A., Cuevas-Díaz, M. D. C., & Vázquez, V. (2019) · SN Applied Sciences · doi:10.1007/s42452-019-0977-3 · Cited by 372 · see citing works
Nutrient Removal by Algae-Based Wastewater Treatment
Nguyen, L. N., Aditya, L., Vu, H. P., Johir, A. H., Bennar, L., Ralph, P. J., Hoang, N. B., Zdarta, J., & Nghiem, L. D. (2022) · Current Pollution Reports · doi:10.1007/s40726-022-00230-x · Cited by 154 · see citing works
AlMomani, F. A., & Bhosale, R. R. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.142654 · Cited by 138 · see citing works
Tran, H. N. (2023) · Water · doi:10.3390/w15061231 · Cited by 115 · see citing works
Nitrate and Nitrite Removal from Wastewater using Algae
Taziki, M., Ahmadzadeh, H., Murry, M. A., & Lyon, S. R. (2015) · Current Biotechnology · doi:10.2174/2211550104666150828193607 · Cited by 111 · see citing works
Nivetha, N., Srivarshine, B., Sowmya, B., Mangaiyarkarasi, R., Saravanan, P., Rajeshkannan, R., Rajasimman, M., Pham, T. H. T., Shanmugam, V., & Drăgoi, E.-N. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.137099 · Cited by 108 · see citing works
Yamashita, T., & Yamamoto, R. (2014) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph110909835 · Cited by 98 · see citing works
Margariti, C. (2019) · Heritage Science · doi:10.1186/s40494-019-0304-8 · Cited by 98 · see citing works
Bhuyar, P., Trejo, M., Dussadee, N., Unpaprom, Y., Ramaraj, R., & Whangchai, K. (2021) · Water Science & Technology · doi:10.2166/wst.2021.194 · Cited by 85 · see citing works
Ramli, A. N. M., Manap, N. W. A., Bhuyar, P., & Azelee, N. I. W. (2020) · SN Applied Sciences · doi:10.1007/s42452-020-03550-z · Cited by 79 · see citing works
Napiórkowska‐Krzebietke, A., Napiórkowska‐Krzebietke, A., Hussian, A.-E. M., El-Monem, A. M. A., Goher, M. E.-S., Abdel-Satar, A. M., & Ali, M. H. H. (2016) · Journal of Elementology · doi:10.5601/jelem.2015.20.4.1037 · Cited by 66 · see citing works
Kandimalla, P., Priyanka, V., Rao, B. C. S., & Raghavender, V. (2022) · Energy Nexus · doi:10.1016/j.nexus.2022.100122 · Cited by 65 · see citing works
Wang, Y., Zhou, Q., Li, B., Liu, B., Wu, G., Ibrahim, M. K., Xie, G., Li, H., & Sun, G. (2012) · BMC Microbiology · doi:10.1186/1471-2180-12-182 · Cited by 57 · see citing works
Abdelhay, A. W., Bsoul, A. A., Al–Othman, A., Al-Ananzeh, N., Jum’h, I., & Al-Taani, A. A. (2017) · Adsorption Science & Technology · doi:10.1177/0263617416684347 · Cited by 57 · see citing works
Challenges and opportunities for third-generation ethanol production: A critical review
Müller, C., Scapini, T., Rempel, A., ABAIDE, E. R., Camargo, A. F., Nazari, M. T., Tadioto, V., Bonatto, C., Tres, M. V., Zabot, G. L., et al. (2022) · Engineering Microbiology · doi:10.1016/j.engmic.2022.100056 · Cited by 51 · see citing works
Duan, J., Feng, Y., Yu, Y., He, S., Xue, L., & Yang, L. (2016) · Environmental Monitoring and Assessment · doi:10.1007/s10661-016-5286-3 · Cited by 41 · see citing works
Utilization of Microalgae in Aquaculture System: Biological Wastewater Treatment
Kim, K., Jung, J.-Y., & Han, H. (2019) · Emerging Science Journal · doi:10.28991/esj-2019-01183 · Cited by 37 · see citing works
Missau, J., Rodrigues, M. A. S., Bertuol, D. A., & Tanabe, E. H. (2022) · Water Science and Technology · doi:10.2166/wst.2022.332 · Cited by 25 · see citing works
Coelho, R. S., Cuellar, M. C., Franco, T. T., & van der Wielen, L. A. M. (2020) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.2174 · Cited by 23 · see citing works
Removal of phosphate and nitrate from aqueous solution using seagrass Cymodocea rotundata beads
G., N. S., Manickavasagam, M., Santhanam, P., Dinesh Kumar, S., & Prabhavathi, P. A. (2015) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb2015.14468 · Cited by 22 · see citing works
Kumaraguru, K., Saravanan, P., Kannan, R. R., & Saravanan, V. (2021) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-01795-1 · Cited by 22 · see citing works
Kasalkar, H. V., & Wagh, N. S. (2022) · Nature Environment and Pollution Technology · doi:10.46488/nept.2022.v21i03.007 · Cited by 18 · see citing works
Lin, X., Luo, L., Mao, Z., Wang, H., Chu, S., Wang, H., & Luo, S. (2022) · Polymers · doi:10.3390/polym14204349 · Cited by 15 · see citing works
Paul, P., Parbat, S., & Aditya, G. (2022) · RSC Advances · doi:10.1039/d2ra03852h · Cited by 15 · see citing works
Nagarajan, L., Kumaraguru, K., Saravanan, P., Rajeshkannan, R., & Rajasimman, M. (2021) · Environmental Technology · doi:10.1080/09593330.2021.1938243 · Cited by 12 · see citing works
Habtegebriel, H., & Valdramidis, V. (2023) · Algal Research · doi:10.1016/j.algal.2023.103205 · Cited by 10 · see citing works
Preparation of Porous Carbon Materials as Adsorbent Materials from Phosphorus-Doped Watermelon Rind
Wei, Y., Li, P., Yang, C., Li, X., Yi, D., & Wu, W. (2023) · Water · doi:10.3390/w15132433 · Cited by 9 · see citing works
Kiss, B., Gergely, S., Salgó, A., & Németh, Á. (2018) · Periodica Polytechnica Chemical Engineering · doi:10.3311/ppch.12863 · Cited by 5 · see citing works
Nguyen, T. A. H., Ngo, H. H., Guo, W., Pham, T. Q., Cao, T. H., & Nguyen, T. H. (2019) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/266/1/012004 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
A Review of Algae-Based Carbon Capture, Utilization, and Storage (Algae-Based CCUS)
Li, G., & Yao, J. (2024) · Gases · doi:10.3390/gases4040024 · Cited by 80 · see citing works
Göncü, S., Uygun, B. Ş., & Atakan, S. (2025) · International Journal of Environmental Science and Technology · doi:10.1007/s13762-025-06380-x · Cited by 34 · see citing works
Salimi, A., Ghanbarizadeh, P., Mirvakili, A., & Moheimani, N. R. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.179938 · Cited by 17 · see citing works
Lead biosorption from industrial wastewater using Cladophora
Metwally, A. M., Aly, S. A., Makled, S. O., & Abdelkader, A. M. (2025) · Scientific Reports · doi:10.1038/s41598-025-29920-4 · Cited by 5 · see citing works
Raza, N., Ali, Z., Manzoor, S., Azzouz, A., Aziz, K., Hashim, S., Khairy, M., Salem, M. E., & Chaudhary, A. A. (2025) · RSC Advances · doi:10.1039/d5ra08065g · Cited by 4 · see citing works
Gincley, B., Khan, F. A., Alam, M. M., Hartnett, E., Kim, G.-Y., Molitor, H. R., Fisher, A., Bradley, I., Guest, J. S., & Pinto, A. J. (2024) · Water Research · doi:10.1016/j.watres.2025.123801 · Cited by 6 · see citing works
Nature-Based Approaches for Managing Bioavailable Phosphorus in Aquatic Ecosystems
Pavlíková, M., Odehnalová, K., Zezulka, Š., Maršálková, E., Lamaczová, A., & Maršálek, B. (2025) · Hydrology · doi:10.3390/hydrology12090236 · Cited by 3 · see citing works
Manjunath, S. V., Girish, C. R., Sreenivasa Murthy, A., Shiva Prasad, N., Manjunath, N. K., R, R. H., Sachidananda, B., Shuvanjan, B., & Sutheerth, A. (2025) · Chemical Engineering Journal Advances · doi:10.1016/j.ceja.2025.100964 · Cited by 3 · see citing works
Doctolero, J. S., Saha, J., Rice, T. A., Hussain, A. S., & Brown, P. B. (2025) · Aquaculture · doi:10.1016/j.aquaculture.2025.743277 · Cited by 2 · see citing works
Zhao, J., Wu, L., Yu, Z., Liu, S., Ma, W., Cui, Y., Hou, W., Wang, L., Tian, X., Sun, Y., et al. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1766843 · Cited by 2 · see citing works
Bala, R., Singh, A., Arora, D., Dhankhar, R., Bala, K., & Chhikara, S. K. (2025) · Environment Conservation Journal · doi:10.36953/ecj.31173008 · Cited by 2 · see citing works
Tenza, N. P., & Mahlambi, P. N. (2025) · Water and Environment Journal · doi:10.1111/wej.70004 · Cited by 2 · see citing works
Nematkhah, A., Nazarinia, M., Mohammadi, T., Zarghami, S., & Mohammadi, M. (2026) · Desalination · doi:10.1016/j.desal.2026.119951 · Cited by 1 · see citing works
Yuan, W., Shu, B., Fu, H., & Wu, Y. (2025) · Environmental Engineering Research · doi:10.4491/eer.2025.419
Evaluation of Nitrate Contamination in Groundwater and Sustainable Solution for Its Removal
Dheeraj, K. R., Shaji, A., Shaji, A. K., Jaison, A., & Krishnankutty, S. V. (2026) · Lecture notes in civil engineering · doi:10.1007/978-3-032-04149-4_16
Low-cost date seed and sawdust-derived biochars for nitrate removal from aqueous solutions
Fazli, E., Ahmadaali, K., Salajegheh, A., & Ghasemi, A. (2026) · Discover Water · doi:10.1007/s43832-026-00345-y
Zhang, R., Wang, Y., Wang, K., & Han, C. (2026) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2026.04.020
Chukwulo, C., Zaky, S. K., Agee, J. T., Chia, M. A., Otogo, R. A., & Uyovbisere, E. E. (2026) · International Journal of Phytoremediation · doi:10.1080/15226514.2026.2688538
Clay−Microalgae Biocomposite for Enhanced Nitrate Removal from Water
Khodadadei, M. T., Mansouri, H., & Salari, H. (2026) · Colloid Journal · doi:10.1134/s1061933x25601192
Ghazal, M. F., Ismael, W. H., Doha, N. M. E., Ali, D. F. I., & Salem, G. M. (2026) · Beni-Suef University Journal of Basic and Applied Sciences · doi:10.1186/s43088-026-00801-7
Bolshanina, S., Piatyshkina, P., Ivchenko, V., Grytsenko, N., & Yanovska, A. (2026) · Environmental Problems · doi:10.23939/ep2026.03.303
Miraboutalebi, S. M., Truong, D. Q., Vigneswaran, S., Naidu, G., & Nguyễn, T. V. (2026) · Water Conservation Science and Engineering · doi:10.1007/s41101-026-00574-1
The Eco-Sustainability of Agricultural Drainage Water Bioremediation by Chlorella VulgarisPreprint
El-Sayed, A. E.-K. B., Reda, M. M., El-Kassas, H., & Mostafa, M. B. M. (2026) · Research Square · doi:10.21203/rs.3.rs-10923460/v1
Ahmad, I., Mahboob, M., & Kumar, L. S. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-51012-0.00003-2
Adhithya, S., Nithya, K., Sathish, A., & Kumar, V. (2026) · Applied Soil Ecology · doi:10.1016/j.apsoil.2026.107561
- Authors
- Asaad, A. A., & Amer, A. S.
- Affiliations
- National Water Research Center (EG)
- Corresponding author
- Asaad, Amany A.
- ORCID
- Amer, Amany S.
- Venue
- Scientific Reports
- Published
- 2024-01-09
- Volume
- 14
- Issue
- 1
- Pages
- 884-884
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC10776767
- OpenAlex
- W4390783593
- Cited by
- 25
- References
- 37
- Citations by year
- 2026: 13 · 2025: 10 · 2024: 2
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Water Research Center
- Topics
- Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
- Physical Sciences › Environmental Science › Waste Management and Disposal › Phosphorus and nutrient management
- Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
- Keywords
- Chlorella vulgaris, biosorption, phosphate removal, nitrate removal, wastewater treatment, microalgae, Freundlich isotherm, pseudo-second-order kinetics, Elovich kinetics, adsorption equilibrium, pH effect, nutrient enrichment
- MeSH terms
- Nitrates, Phosphates, Phosphorus, Chlorella vulgaris, Wastewater
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
