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Cited by 21Mesenchymal stem cell researchExtracellular vesicles in diseasePluripotent Stem Cells Research

Unveiling the Immunomodulatory and regenerative potential of iPSC-derived mesenchymal stromal cells and their extracellular vesicles

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Buitrago, July Constanza, Morris, Sarah L., Backhaus, Astrid, Kaltenecker, Gesa, Kaipa, Jagan Mohan, Girard, Cyrille, Schneider, Stefan, Gruber, Jens·2024·Scientific Reports
DOI 10.1038/s41598-024-75956-3PMC PMC11480492
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Abstract

Induced pluripotent stem cell (iPSC)-derived mesenchymal stromal cells (iMSCs) offer a promising alternative to primary mesenchymal stromal cells (MSCs) and their derivatives, particularly extracellular vesicles (EVs), for use in advanced therapy medicinal products. In this study we evaluated the immunomodulatory and regenerative potential of iMSCs as well as iMSC-EVs, alongside primary human umbilical cord-derived mesenchymal stromal cells (hUCMSCs). Our findings demonstrate that iMSCs exhibit comparable abilities to hUCMSCs in regulating lymphocyte proliferation and inducing an anti-inflammatory phenotype in monocytes. We also observed decreased TNFα levels and increased IL-10 induction, indicating a potential mechanism for their immunomodulatory effects. Furthermore, iMSC-EVs also showed effective immunomodulation by inhibiting T cell proliferation and inducing macrophage polarization similar to their parental cells. Additionally, iMSC-EVs exhibited pro-regenerative potential akin to hUCMSC-EVs in in vitro scratch assays. Notably, priming iMSCs with pro-inflammatory cytokines significantly enhanced the immunomodulatory potential of iMSC-EVs. These results underscore the considerable promise of iMSCs and iMSCs-EVs as an alternate source for MSC-derived therapeutics, given their potent immunomodulatory and regenerative properties.

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Authors
Buitrago, J. C., Morris, S. L., Backhaus, A., Kaltenecker, G., Kaipa, J. M., Girard, C., Schneider, S., & Gruber, J.
Affiliations
Universidad del Rosario (CO)
Corresponding author
  • Buitrago, July Constanza
  • Gruber, Jens
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2024-10-15
Volume
14
Issue
1
Pages
24098-24098
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-024-75956-3
PMC
PMC11480492
OpenAlex
W4403438509
Cited by
21
References
1
Citations by year
2026: 11 · 2025: 10
FWCI
5.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by-nc-nd)
Funders
Universidad del Rosario
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
extracellular vesicles, immunomodulation, regenerative capacity, lymphocyte proliferation, macrophage polarization, T cell proliferation, IL-10, TNF-α, advanced therapy medicinal products
MeSH terms
Extracellular Vesicles, Cell Differentiation, Cells, Cultured, Humans, Macrophages, Monocytes, Umbilical Cord, Cytokines, Cell Proliferation, Immunomodulation, Induced Pluripotent Stem Cells, Mesenchymal Stem Cells

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