Cited by 199RNA modifications and cancerRNA and protein synthesis mechanismsNuclear Structure and Function

Architecture and assembly of mammalian H/ACA small nucleolar and telomerase ribonucleoproteins

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Wang, Chen, Meier, U Thomas·2004·The EMBO Journal

Abstract

Mammalian H/ACA small nucleolar RNAs and telomerase RNA share common sequence and secondary structure motifs that form ribonucleoprotein particles (RNPs) with the same four core proteins, NAP57 (also dyskerin or in yeast Cbf5p), GAR1, NHP2, and NOP10. The assembly and molecular interactions of the components of H/ACA RNPs are unknown. Using in vitro transcription/translation in combination with immunoprecipitation of core proteins, UV-crosslinking, and electrophoretic mobility shift assays, we demonstrate the following. NOP10 associates with NAP57 as a prerequisite for NHP2 binding. Although NHP2 on its own binds RNA nonspecifically, this NAP57-NOP10-NHP2 core trimer specifically recognizes H/ACA RNAs. GAR1 associates independently with NAP57 near the pseudouridylase core of mature H/ACA RNPs. In contrast to other RNPs whose assembly is initiated by protein-RNA interactions, the four H/ACA core proteins form a protein-only particle that associates with H/ACA RNAs. Nonetheless, functional H/ACA snoRNPs assembled in cytosolic extracts are stable and do not exchange their RNA components, suggesting that new particle formation requires de novo synthesis.

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Authors
Wang, C., & Meier, U. T.
Affiliations
Albert Einstein College of Medicine (US)
Corresponding author
Meier, U Thomas
Venue
The EMBO Journal
Published
2004-03-25
Volume
23
Issue
8
Pages
1857-1867
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W2122834552
Cited by
199
References
67
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 12 · 2022: 6 · 2021: 19 · 2020: 4 · 2019: 7 · 2018: 6 · 2017: 4 · 2016: 10 · 2015: 7 · 2014: 7 · 2013: 9 · 2012: 11
FWCI
6.41
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
American Cancer Society
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
telomerase RNA, ribonucleoprotein assembly, dyskerin, NOP10, NHP2, GAR1, RNA-protein interactions, RNA recognition, snoRNA processing, telomerase
MeSH terms
Animals, Cell Extracts, Humans, Mammals, Mutation, Nuclear Proteins, Protein Binding, RNA, Ultraviolet Rays, Uridine, RNA-Binding Proteins, Ribonucleoproteins, Small Nuclear, Telomerase, RNA, Small Nucleolar, Ribonucleoproteins, Small Nucleolar, Rats

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