The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
The inner nuclear membrane protein Emerin regulates β‐catenin activity by restricting its accumulation in the nucleus
Open accessAbstract
Emerin is a type II inner nuclear membrane (INM) protein of unknown function. Emerin function is likely to be important because, when it is mutated, emerin promotes both skeletal muscle and heart defects. Here we show that one function of Emerin is to regulate the flux of beta-catenin, an important transcription coactivator, into the nucleus. Emerin interacts with beta-catenin through a conserved adenomatous polyposis coli (APC)-like domain. When GFP-emerin is expressed in HEK293 cells, beta-catenin is restricted to the cytoplasm and beta-catenin activity is inhibited. In contrast, expression of an emerin mutant, lacking its APC-like domain (GFP-emerinDelta), dominantly stimulates beta-catenin activity and increases nuclear accumulation of beta-catenin. Human fibroblasts that are null for emerin have an autostimulatory growth phenotype. This unusual growth phenotype arises through enhanced nuclear accumulation and activity of beta-catenin and can be replicated in wild-type fibroblasts by transfection with constitutively active beta-catenin. Our results support recent findings that suggest that INM proteins can influence signalling pathways by restricting access of transcription coactivators to the nucleus.
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“Emerin is a structural integral protein which acts to stabilize chromatin and regulate gene expression [108].”
From DNA repair deficiency and senescence in concussed professional athletes involved in contact sports (2019) · cites this paper as [108]
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Citations
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Metadata
- Authors
- Markiewicz, E., Tilgner, K., Barker, N., van de Wetering, M., Clevers, H., Dorobek, M., Hausmanowa‐Petrusewicz, I., Ramaekers, F. C., Broers, J. L., Blankesteijn, W. M., Salpingidou, G., Wilson, R. G., Ellis, J. A., & Hutchison, C. J.
- Affiliations
- Durham University (GB)
- Hubrecht Institute for Developmental Biology and Stem Cell Research (NL)
- Polish Academy of Sciences (PL)
- Maastricht University (NL)
- James Cook University Hospital (GB)
- King's College London (GB)
- Corresponding author
- Hutchison, Christopher J
- ORCID
- Venue
- The EMBO Journal
- Published
- 2006-07-20
- Volume
- 25
- Issue
- 14
- Pages
- 3275-3285
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC1523183
- OpenAlex
- W2115754063
- Cited by
- 246
- References
- 57
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 6 · 2023: 5 · 2022: 10 · 2021: 10 · 2020: 15 · 2019: 10 · 2018: 13 · 2017: 12 · 2016: 17 · 2015: 16 · 2014: 18 · 2013: 13 · 2012: 17
- FWCI
- 5.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- TU/e Research Portal (publishedVersion)
- Durham Research Online (Durham University) (acceptedVersion)
- TU/e Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Durham Research Online (Durham University) (submittedVersion)
- Durham Research Online (Durham University) (submittedVersion, cc-by)
- Research Portal (King's College London) (submittedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (submittedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Funders
- Wellcome Trust
- Royal Society
- Muscular Dystrophy UK
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Keywords
- emerin, inner nuclear membrane, β-catenin, nuclear accumulation, transcriptional coactivator, nuclear trafficking, Wnt signaling
- MeSH terms
- Cell Line, Cells, Cultured, Humans, Membrane Proteins, Nuclear Envelope, Nuclear Proteins, Thymopoietins, Signal Transduction, Trans-Activators, Muscular Dystrophy, Emery-Dreifuss, beta Catenin
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