The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Detecting cAMP‐induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator
Open accessAbstract
Epac1 is a guanine nucleotide exchange factor for Rap1 that is activated by direct binding of cAMP. In vitro studies suggest that cAMP relieves the interaction between the regulatory and catalytic domains of Epac. Here, we monitor Epac1 activation in vivo by using a CFP–Epac–YFP fusion construct. When expressed in mammalian cells, CFP–Epac–YFP shows significant fluorescence resonance energy transfer (FRET). FRET rapidly decreases in response to the cAMP‐raising agents, whereas it fully recovers after addition of cAMP‐lowering agonists. Thus, by undergoing a cAMP‐induced conformational change, CFP–Epac–YFP serves as a highly sensitive cAMP indicator in vivo . When compared with a protein kinase A (PKA)‐based sensor, Epac‐based cAMP probes show an extended dynamic range and a better signal‐to‐noise ratio; furthermore, as a single polypeptide, CFP–Epac–YFP does not suffer from the technical problems encountered with multisubunit PKA‐based sensors. These properties make Epac‐based FRET probes the preferred indicators for monitoring cAMP levels in vivo .
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Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP
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Self-citationDiscrete Microdomains with High Concentration of cAMP in Stimulated Rat Neonatal Cardiac Myocytes
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Self-citationThe structural basis of the activation of Ras by Sos
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Fluorescence ratio imaging of cyclic AMP in single cells
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Novel Single Chain cAMP Sensors for Receptor-induced Signal Propagation
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A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK
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Self-citationSpatio-temporal images of growth-factor-induced activation of Ras and Rap1
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Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in Aplysia Sensory Neurons
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Epac: a new cAMP target and new avenues in cAMP research
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Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Fluorescent protein FRET: the good, the bad and the ugly
Piston, D. W., & Kremers, G.-J. (2007) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.08.003 · Cited by 849
Epac proteins: multi-purpose cAMP targets
Bos, J. L. (2006) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2006.10.002 · Cited by 532
Self-citationCyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte
Norris, R. P., Ratzan, W. J., Freudzon, M., Mehlmann, L. M., Krall, J., Movsesian, M. A., Wang, H., Ke, H., Nikolaev, V. O., & Jaffe, L. A. (2009) · Development · doi:10.1242/dev.035238 · Cited by 516
Connexin channel permeability to cytoplasmic molecules
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Metadata
- Authors
- Ponsioen, B., Zhao, J., Riedl, J., Zwartkruis, F., van der Krogt, G., Zaccolo, M., Moolenaar, W. H., Bos, J. L., & Jalink, K.
- Venue
- EMBO reports
- Published
- 2004-12
- Volume
- 5
- Issue
- 12
- Pages
- 1176-1180
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2126704964
- Cited by
- 444
- References
- 21
- FWCI
- 9.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Ponsioen, B., Zhao, J., Riedl, J., Zwartkruis, F., van der Krogt, G., Zaccolo, M., Moolenaar, W. H., Bos, J. L., & Jalink, K. (2004). Detecting cAMP‐induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator. EMBO reports. https://doi.org/10.1038/sj.embor.7400290
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