Cited by 799Protein Kinase Regulation and GTPase SignalingReceptor Mechanisms and SignalingProtein Tyrosine Phosphatases

The structural basis of the activation of Ras by Sos

Boriack-Sjodin, P. Ann, Margarit, S. Mariana, Bar-Sagi, Dafna, Kuriyan, John·1998·Nature

Abstract

The crystal structure of human H-Ras complexed with the Ras guanine-nucleotide-exchange-factor region of the Son of sevenless (Sos) protein has been determined at 2.8 A resolution. The normally tight interaction of nucleotides with Ras is disrupted by Sos in two ways. First, the insertion into Ras of an alpha-helix from Sos results in the displacement of the Switch 1 region of Ras, opening up the nucleotide-binding site. Second, side chains presented by this helix and by a distorted conformation of the Switch 2 region of Ras alter the chemical environment of the binding site for the phosphate groups of the nucleotide and the associated magnesium ion, so that their binding is no longer favoured. Sos does not impede the binding sites for the base and the ribose of GTP or GDP, so the Ras-Sos complex adopts a structure that allows nucleotide release and rebinding.

Integrity signals

Registry

Crossref · 10.1038/28548

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Boriack-Sjodin, P. A., Margarit, S. M., Bar-Sagi, D., & Kuriyan, J.
Affiliations
  • State University of New York (US)
  • Howard Hughes Medical Institute (US)
Venue
Nature
Published
1998-07
Volume
394
Issue
6691
Pages
337-343
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W1824220821
Cited by
799
References
46
Citations by year
2026: 17 · 2025: 34 · 2024: 37 · 2023: 28 · 2022: 40 · 2021: 27 · 2020: 33 · 2019: 36 · 2018: 24 · 2017: 30 · 2016: 25 · 2015: 28 · 2014: 26 · 2013: 29 · 2012: 15
FWCI
15.82
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
Keywords
Ras activation, SO, Son of Sevenless, H-Ras, guanine nucleotide exchange factor, crystal structure, nucleotide binding site, GTP binding, GDP binding, nucleotide release
MeSH terms
Binding Sites, Catalysis, Cloning, Molecular, Escherichia coli, Guanine, Guanosine Triphosphate, Humans, Membrane Proteins, Models, Molecular, Molecular Sequence Data, Mutation, Peptide Elongation Factor Tu, Peptide Elongation Factors, Protein Binding, Protein Conformation, Structure-Activity Relationship, Crystallography, X-Ray, ras Proteins, Son of Sevenless Proteins, GTP Phosphohydrolase-Linked Elongation Factors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review