The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter
Open accessAbstract
Kaede is a natural photoconvertible fluorescent protein found in the coral Trachyphyllia geoffroyi . It contains a tripeptide, His 62‐Tyr 63‐Gly 64, which acts as a green chromophore that is photoconvertible to red following (ultra‐) violet irradiation. Here, we report the molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus , and its in vitro evolution conferring green‐to‐red photoconvertibility. Substitution of the His 62‐Tyr 63‐Gly 64 sequence into the native protein provided only negligible photoconversion. On the basis of the crystal structure, semi‐rational mutagenesis of the amino acids surrounding the chromophore was performed, leading to the generation of an efficient highlighter, KikGR. Within mammalian cells, KikGR is more efficiently photoconverted and is several‐fold brighter in both the green and red states than Kaede. In addition, KikGR was successfully photoconverted using two‐photon excitation microscopy at 760 nm, ensuring optical cell labelling with better spatial discrimination in thick and highly scattering tissues.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Tsien, R. Y. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.509 · Cited by 6,143
Crystal Structure of the Aequorea victoria Green Fluorescent Protein
Ormö, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996) · Science · doi:10.1126/science.273.5280.1392 · Cited by 2,242
Creating new fluorescent probes for cell biology
Zhang, J., Campbell, R. E., Ting, A. Y., & Tsien, R. Y. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm976 · Cited by 1,981
Fluorescent proteins from nonbioluminescent Anthozoa species
Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L., & Lukyanov, S. A. (1999) · Nature Biotechnology · doi:10.1038/13657 · Cited by 1,838
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
Patterson, G. H., & Lippincott-Schwartz, J. (2002) · Science · doi:10.1126/science.1074952 · Cited by 1,612
The molecular structure of green fluorescent protein
Yang, F., Moss, L. G., & Phillips, G. N. (1996) · Nature Biotechnology · doi:10.1038/nbt1096-1246 · Cited by 1,576
An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., & Miyawaki, A. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.202320599 · Cited by 1,027
Self-citationEosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion
Wiedenmann, J., Ivanchenko, S., Oswald, F., Schmitt, F., Röcker, C., Salih, A., Spindler, K.-D., & Nienhaus, G. U. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0403668101 · Cited by 678
Photoswitchable cyan fluorescent protein for protein tracking
Chudakov, D. M., Verkhusha, V. V., Staroverov, D. B., Souslova, E. A., Lukyanov, S., & Lukyanov, K. A. (2004) · Nature Biotechnology · doi:10.1038/nbt1025 · Cited by 361
Sawano, A. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.16.e78 · Cited by 351
Cited by
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Fluorescent probes for super-resolution imaging in living cells
Fernández-Suárez, M., & Ting, A. Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2531 · Cited by 1,306
Principles of two-photon excitation microscopy and its applications to neuroscience
Svoboda, K., & Yasuda, R. (2006) · Neuron · doi:10.1016/j.neuron.2006.05.019 · Cited by 1,027
Advances in fluorescent protein technology
Shaner, N. C., Patterson, G. H., & Davidson, M. W. (2007) · Journal of Cell Science · doi:10.1242/jcs.005801 · Cited by 787
Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light
Gurskaya, N. G., Verkhusha, V. V., Shcheglov, A. S., Staroverov, D. B., Chepurnykh, T. V., Fradkov, A. F., Lukyanov, S., & Lukyanov, K. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1191 · Cited by 725
Metadata
- Authors
- Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., & Miyawaki, A.
- Venue
- EMBO reports
- Published
- 2005-03
- Volume
- 6
- Issue
- 3
- Pages
- 233-238
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2140588274
- Cited by
- 326
- References
- 15
- FWCI
- 12.58
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter. https://app.cochat.ai/papers/10.1038/sj.embor.7400361
- The paper
- Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., & Miyawaki, A. (2005). Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO reports. https://doi.org/10.1038/sj.embor.7400361
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
