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A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
Abstract
We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange.
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Metadata
- Authors
- Patterson, G. H., & Lippincott-Schwartz, J.
- Venue
- Science
- Published
- 2002-09-13
- Volume
- 297
- Issue
- 5588
- Pages
- 1873-1877
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1988326309
- Cited by
- 1,612
- References
- 0
- FWCI
- 32.99
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
