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The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Rapid and highly efficient transduction by double-stranded adeno-associated virus vectors in vitro and in vivo

Wang, Z, Ma, H-I, Li, J, Sun, L, Zhang, J, Xiao, X·2003·Gene Therapy
DOI 10.1038/sj.gt.3302133
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Abstract

Adeno-associated virus (AAV) is a promising gene vector based on a single-stranded (ss) DNA virus. Its transgene expression requires the conversion of ssDNA to double-stranded (ds) genome, a slow process responsible for the delayed transduction and occasional inefficiency. By mutating the inverted terminal repeat, we have made novel AAV vectors that predominantly package the self-complementary dsDNA genome. The dsAAV consistently demonstrated superior and accelerated transduction in vitro and in vivo. Dramatic increases in transgene expression were observed in most of the cell lines examined, including B16 melanoma and 3LL lung cancer that are difficult to be transduced by the conventional ssAAV vectors. Similar increases were also observed in vivo in a variety of tissues including muscle and liver. The dsAAV transduced a vast majority of the hepatocytes for more than 6 months, while the ssAAV transduced only a small fraction. In addition to circumventing the requirement for DNA synthesis, the dsAAV exhibited higher in vivo DNA stability and more effective circularization than the ssAAV, suggesting potential molecular mechanisms for the faster, stronger and prolonged transgene expression.

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Research works printed but not linked: 25

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Metadata

Authors
Wang, Z., Ma, H.-I., Li, J., Sun, L., Zhang, J., & Xiao, X.
Venue
Gene Therapy
Publisher
Springer Science and Business Media LLC
Published
2003-11-17
Volume
10
Issue
26
Pages
2105-2111
ISSN
  • 0969-7128
  • 1476-5462
DOI
10.1038/sj.gt.3302133
Cited by
367
References
6
Language
en
Licence

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CoChat. (2026). Citation check: Rapid and highly efficient transduction by double-stranded adeno-associated virus vectors in vitro and in vivo. https://app.cochat.ai/papers/10.1038/sj.gt.3302133
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Wang, Z., Ma, H.-I., Li, J., Sun, L., Zhang, J., & Xiao, X. (2003). Rapid and highly efficient transduction by double-stranded adeno-associated virus vectors in vitro and in vivo. Gene Therapy. https://doi.org/10.1038/sj.gt.3302133