DNA helicase‐mediated packaging of adeno‐associated virus type 2 genomes into preformed capsids
Open accessAbstract
Helicases not only catalyse the disruption of hydrogen bonding between complementary regions of nucleic acids, but also move along nucleic acid strands in a polar fashion. Here we show that the Rep52 and Rep40 proteins of adeno-associated virus type 2 (AAV-2) are required to translocate capsid-associated, single-stranded DNA genomes into preformed empty AAV-2 capsids, and that the DNA helicase function of Rep52/40 is essential for this process. Furthermore, DNase protection experiments suggest that insertion of AAV-2 genomes proceeds from the 3' end, which correlates with the 3'-->5' processivity demonstrated for the Rep52/40 helicase. A model is proposed in which capsid-immobilized helicase complexes act as molecular motors to 'pump' single-stranded DNA across the capsid boundary.
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After one single-stranded genome is fully displaced, the DNA is translocated into the capsid in a 3′ to 5′ direction by the helicase/ATPase domain of the small Rep proteins.
“After one single-stranded genome is fully displaced, the DNA is reported to be translocated into the capsid in a 3′ to 5′ direction by the helicase/ATPase domain of the small Rep proteins [53].”
From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [53]
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- Authors
- King, J. A., Dubielzig, R., Grimm, D., & Kleinschmidt, J. A.
- Affiliations
- German Cancer Research Center (DE)
- Medigene (Germany) (DE)
- Corresponding author
- Kleinschmidt, Jürgen A.
- Venue
- The EMBO Journal
- Published
- 2001-06-15
- Volume
- 20
- Issue
- 12
- Pages
- 3282-3291
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC150213
- OpenAlex
- W2051072233
- Cited by
- 338
- References
- 3
- Citations by year
- 2026: 13 · 2025: 25 · 2024: 24 · 2023: 15 · 2022: 10 · 2021: 21 · 2020: 12 · 2019: 16 · 2018: 9 · 2017: 14 · 2016: 12 · 2015: 13 · 2014: 10 · 2013: 15 · 2012: 9
- FWCI
- 6.01
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Keywords
- adeno-associated virus type 2, DNA packaging, DNA helicase, single-stranded DNA, molecular motors, viral genome assembly
- MeSH terms
- Dependovirus, Capsid, Cell Line, Transformed, DNA Helicases, DNA-Binding Proteins, DNA, Viral, Humans, Viral Proteins, Genome, Viral, Virus Assembly
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