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Normal proportion and expression of maturation markers in migratory dendritic cells in the absence of germs or Toll-like receptor signaling
Abstract
Dendritic cells (DCs) play major roles in immunosurveillance. In peripheral tissues, 'immature' DCs are dedicated to capturing antigens. Detection of pathogens through Toll-like receptors (TLRs) triggers DC migration to the lymph nodes (LNs), where they acquire a 'mature' phenotype specialized at presenting antigens. However, DCs migrate from tissues and mature even in the absence of overt infections. This has been attributed to detection of commensal flora in the skin, the gut or other peripheral tissues in the steady state. To test this assumption, we have analyzed the DCs contained in the lymphoid organs of germ-free mice and of mice lacking the TLR adapter molecules, MyD88 and TRIF. We show that the proportion and expression of maturation markers in DC immigrants in the LNs of these mice are similar to those in normal mice. These results suggest that DC migration from tissues, followed by their phenotypic maturation, is regulated in the steady state by an inherent program of DC differentiation or by the release of low levels of inflammatory signals from normal tissues.
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“Of note, we did not observe differences in DC proportions in the spleen, tumor-draining or inguinal lymph nodes from SPF and GF mice that were either naive or tumor-bearing (Figures S2D and S2E), agreeing with previous reports (Ganal et al., 2012; Wilson et al., 2008).”
From Microbiota triggers STING-type I IFN-dependent monocyte reprogramming of the tumor microenvironment (2021) · cites this paper as Wilson et al., 2008
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Metadata
- Authors
- Wilson, N. S., Young, L. J., Kupresanin, F., Naik, S. H., Vremec, D., Heath, W. R., Akira, S., Shortman, K., Boyle, J., Maraskovsky, E., Belz, G. T., & Villadangos, J. A.
- Affiliations
- Genentech (US)
- Walter and Eliza Hall Institute of Medical Research (AU)
- Research Institute for Microbial Diseases, The University of Osaka (JP)
- CSL (Australia) (AU)
- The Walter and Eliza Hall Institute of Medical Research Parkville Victoria Australia
- Corresponding author
- Belz, Gabrielle T
- ORCID
- Venue
- Immunology & Cell Biology
- Publisher
- Wiley
- Published
- 2007-11-20
- Volume
- 86
- Issue
- 2
- Pages
- 200-205
- ISSN
- 0004-945X (ISSN-L)
- 0818-9641
- 1440-1711
- OpenAlex
- W1969532079
- Cited by
- 102
- References
- 38
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 10 · 2016: 5 · 2015: 8 · 2014: 7 · 2013: 9 · 2012: 8
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Immunology and Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Health and Medical Research Council of Australia
- University of Melbourne Research Scholarship
- Leukemia and Lymphoma Society Scholarship
- Wellcome Trust Senior Overseas Fellowship
- Howard Hughes Medical Institute International Fellowships
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- dendritic cells, dendritic cell migration, dendritic cell maturation, Toll-like receptors, MyD88, TRIF, germ-free mice, lymph nodes, steady-state immunity, commensal microbiota, inflammatory signals
- MeSH terms
- Animals, Bacteria, Cell Movement, Dendritic Cells, Germ-Free Life, Mice, Inbred C57BL, Signal Transduction, Adaptor Proteins, Vesicular Transport, Toll-Like Receptors, Mice, Myeloid Differentiation Factor 88
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