High resolution X-ray computed tomography: an emerging tool for small animal cancer research
Open accessAbstract
Dedicated high-resolution small animal imaging systems have recently emerged as important new tools for cancer research. These new imaging systems permit researchers to noninvasively screen animals for mutations or pathologies and to monitor disease progression and response to therapy. One imaging modality, X-ray microcomputed tomography (microCT) shows promise as a cost-effective means for detecting and characterizing soft-tissue structures, skeletal abnormalities, and tumors in live animals. MicroCT systems provide high-resolution images (typically 50 microns or less), rapid data acquisition (typically 5 to 30 minutes), excellent sensitivity to skeletal tissue and good sensitivity to soft tissue, particularly when contrast-enhancing media are employed. The development of microCT technology for small animal imaging is reviewed, and key considerations for designing small animal microCT imaging protocols are summarized. Recent studies on mouse prostate, lung and bone tumor models are overviewed.
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The dose of radiation in both CT and PET that would need to be delivered to the fish to achieve adequate resolution may have independent biological effects.
“Another concern is the dose of radiation in both CT and PET that would need to be delivered to the fish to achieve adequate resolution, which may have independent biological effects (Paulus et al., 2000; Pickhardt et al., 2005”
From Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis (2008) · cites this paper as Paulus et al., 2000
The Radiation Dose section calls radiation exposure an important concern in microCT studies and compares the estimated dose in mice with published lethal-dose values; Table 1 reports organ doses for high-resolution and screening scans. This supports a concern about CT radiation, but the paper does not establish the dose needed for adequate resolution in fish or address biological effects from PET imaging. (LLM, conf=0.82)
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- Authors
- Paulus, M. J., Gleason, S. S., Kennel, S. J., Hunsicker, P. R., & Johnson, D. K.
- Affiliations
- Oak Ridge National Laboratory (US)
- Corresponding author
- Paulus, Michael J.
- ORCID
- Paulus, Michael J.
- Venue
- Neoplasia
- Publisher
- Elsevier BV
- Published
- 2000-01
- Volume
- 2
- Issue
- 1-2
- Pages
- 62-70
- ISSN
- 1476-5586 (ISSN-L)
- PMC
- PMC1531867
- OpenAlex
- W2044754414
- Cited by
- 490
- References
- 2
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 6 · 2023: 9 · 2022: 10 · 2021: 15 · 2020: 16 · 2019: 13 · 2018: 24 · 2017: 22 · 2016: 21 · 2015: 12 · 2014: 16 · 2013: 21 · 2012: 30
- FWCI
- 3.32
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Neoplasia (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- U.S. Department of Energy
- Laboratory Directed Research and Development
- Oak Ridge National Laboratory
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Physical Sciences › Engineering › Biomedical Engineering › Advanced X-ray and CT Imaging
- Keywords
- micro-computed tomography, small animal imaging, tumor detection, non-invasive imaging, imaging protocols, bone tumors, skeletal abnormalities, soft tissue imaging
- MeSH terms
- Animals, Diagnostic Imaging, Neoplasms, Experimental, Tomography, X-Ray Computed, X-Rays, Models, Statistical, Mice
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