The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
8-oxoguanine causes spontaneous de novo germline mutations in mice
Open accessAbstract
Spontaneous germline mutations generate genetic diversity in populations of sexually reproductive organisms and are thus regarded as a driving force of evolution. However, the cause and mechanism remain unclear. 8-oxoguanine (8-oxoG) is a candidate molecule that causes germline mutations, because it makes DNA more prone to mutation and is constantly generated by reactive oxygen species in vivo. We show here that endogenous 8-oxoG caused de novo spontaneous and heritable G to T mutations in mice, which occurred at different stages in the germ cell lineage and were distributed throughout the chromosomes. Using exome analyses covering 40.9 Mb of mouse transcribed regions, we found increased frequencies of G to T mutations at a rate of 2 × 10−7 mutations/base/generation in offspring of Mth1/Ogg1/Mutyh triple knockout (TOY-KO) mice, which accumulate 8-oxoG in the nuclear DNA of gonadal cells. The roles of MTH1, OGG1 and MUTYH are specific for the prevention of 8-oxoG-induced mutation and 99% of the mutations observed in TOY-KO mice were G to T transversions caused by 8-oxoG; therefore, we concluded that 8-oxoG is a causative molecule for spontaneous and inheritable mutations of the germ lineage cells.
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Cites
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224
Rate of de novo mutations and the importance of father’s age to disease risk
Kong, A., Frigge, M. L., Masson, G., Besenbacher, S., Sulem, P., Magnusson, G., Gudjonsson, S. A., Sigurdsson, A., Jonasdottir, A., Jonasdottir, A., et al. (2012) · Nature · doi:10.1038/nature11396 · Cited by 2,134
Drake, J. W., Charlesworth, B., Charlesworth, D., & Crow, J. F. (1998) · Genetics · doi:10.1093/genetics/148.4.1667 · Cited by 1,932
Xie, Y., Yang, H., Cunanan, C., Okamoto, K., Shibata, D., Pan, J., Barnes, D. E., Lindahl, T., McIlhatton, M., Fishel, R., et al. (2004) · Cancer Research · doi:10.1158/0008-5472.can-03-3834 · Cited by 291
Keays, D. A., Clark, T. G., Campbell, T. G., Broxholme, J., & Valdar, W. (2007) · Mammalian Genome · doi:10.1007/s00335-006-0065-z · Cited by 77
Keays, D. A., Clark, T. G., & Flint, J. (2006) · Mammalian Genome · doi:10.1007/s00335-005-0101-4 · Cited by 55
Cited by
Causes and consequences of oxidative stress in spermatozoa
Aitken, R. J., Gibb, Z., Baker, M. A., Drevet, J., & Gharagozloo, P. (2015) · Reproduction, Fertility and Development · doi:10.1071/rd15325 · Cited by 428
8-Oxoguanine: from oxidative damage to epigenetic and epitranscriptional modification
Hahm, J. Y., Park, J., Jang, E.-S., & Chi, S. W. (2022) · Experimental & Molecular Medicine · doi:10.1038/s12276-022-00822-z · Cited by 317
Determinants of Mutation Rate Variation in the Human Germline
Ségurel, L., Wyman, M. J., & Przeworski, M. (2014) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev-genom-031714-125740 · Cited by 289
Xavier, M. J., Roman, S. D., Aitken, R. J., & Nixon, B. (2019) · Human Reproduction Update · doi:10.1093/humupd/dmz017 · Cited by 257
Overlooked roles of DNA damage and maternal age in generating human germline mutations
Gao, Z., Moorjani, P., Sasani, T. A., Pedersen, B. S., Quinlan, A. R., Jorde, L. B., Amster, G., & Przeworski, M. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1901259116 · Cited by 229
“In cattle and humans, the high-frequency mosaic mutations that are likely to have arisen in early embryonic development are enriched for C >A transversions (53, 55, 57), potentially reflecting the accumulation of the oxidative DNA damage 8-hydroxyguanine in oocytes and the last stages of spermatogenesis (67, 68, 71) t…”
Background
Metadata
- Authors
- Ohno, M., Sakumi, K., Fukumura, R., Furuichi, M., Iwasaki, Y., Hokama, M., Ikemura, T., Tsuzuki, T., Gondo, Y., & Nakabeppu, Y.
- Venue
- Scientific Reports
- Published
- 2014-04-15
- Volume
- 4
- Issue
- 1
- Pages
- 4689-4689
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W1985357654
- Cited by
- 176
- References
- 6
- FWCI
- 5.23
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ohno, M., Sakumi, K., Fukumura, R., Furuichi, M., Iwasaki, Y., Hokama, M., Ikemura, T., Tsuzuki, T., Gondo, Y., & Nakabeppu, Y. (2014). 8-oxoguanine causes spontaneous de novo germline mutations in mice. Scientific Reports. https://doi.org/10.1038/srep04689
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