Liquid chromatography-mass spectrometry platform for both small neurotransmitters and neuropeptides in blood, with automatic and robust solid phase extraction
Open accessAbstract
Neurons communicate via chemical signals called neurotransmitters (NTs). The numerous identified NTs can have very different physiochemical properties (solubility, charge, size etc.), so quantification of the various NT classes traditionally requires several analytical platforms/methodologies. We here report that a diverse range of NTs, e.g. peptides oxytocin and vasopressin, monoamines adrenaline and serotonin and amino acid GABA, can be simultaneously identified/measured in small samples, using an analytical platform based on liquid chromatography and high-resolution mass spectrometry (LC-MS). The automated platform is cost-efficient as manual sample preparation steps and one-time-use equipment are kept to a minimum. Zwitter-ionic HILIC stationary phases were used for both on-line solid phase extraction (SPE) and liquid chromatography (capillary format, cLC). This approach enabled compounds from all NT classes to elute in small volumes producing sharp and symmetric signals and allowing precise quantifications of small samples, demonstrated with whole blood (100 microliters per sample). An additional robustness-enhancing feature is automatic filtration/filter back-flushing (AFFL), allowing hundreds of samples to be analyzed without any parts needing replacement. The platform can be installed by simple modification of a conventional LC-MS system.
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In Prader-Willi syndrome, Tauber and colleagues observed significant improvements in trusting behaviours, reductions in sad mood, and less disruptive behaviour in the two days following a single dose of oxytocin in 24 adults, but a second study found no significant changes in developmental or emotional behaviours and an increase in temper outbursts.
“Tauber and colleagues observed significant improvements in trusting behaviours, reductions in sad mood, and less disruptive behaviour in the two days following a single dose of oxytocin in 24 adults with PWS.61 A second study of PWS tested effects of twice-daily oxytocin administration over a two month period, in a crossover placebo-controlled design in 30 adolescents and adults with PWS, aged”
From Beyond the hype and hope: critical considerations for intranasal oxytocin research in autism spectrum disorder (2017) · cites this paper as [61]
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Lefevre, A., Mottolese, R., Dirheimer, M., Mottolese, C., Duhamel, J.-R., & Sirigu, A. (2017) · Scientific Reports · doi:10.1038/s41598-017-17674-7 · Cited by 105 · see citing works
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- Authors
- Johnsen, E., Leknes, S., Wilson, S. R., & Lundanes, E.
- Affiliations
- University of Oslo (NO)
- Oslo University Hospital (NO)
- Corresponding author
- Johnsen, Elin
- Wilson, Steven Ray
- Venue
- Scientific Reports
- Published
- 2015-03-20
- Volume
- 5
- Issue
- 1
- Pages
- 9308-9308
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5380133
- OpenAlex
- W2092379136
- Cited by
- 65
- References
- 41
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 10 · 2018: 11 · 2017: 8 · 2016: 5 · 2015: 4
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- NORA - Norwegian Open Research Archives (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- neurotransmitters, neuropeptides, whole blood analysis, automated solid-phase extraction, capillary liquid chromatography, high-resolution mass spectrometry, oxytocin, vasopressin, epinephrine, serotonin, GABA, monoamines
- MeSH terms
- Automation, Chromatography, Liquid, Humans, Neuropeptides, Mass Spectrometry, Neurotransmitter Agents, Solid Phase Extraction
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