Engineering a Brain Cancer Chip for High-throughput Drug Screening
Open accessAbstract
Glioblastoma multiforme (GBM) is the most common and malignant of all human primary brain cancers, in which drug treatment is still one of the most effective treatments. However, existing drug discovery and development methods rely on the use of conventional two-dimensional (2D) cell cultures, which have been proven to be poor representatives of native physiology. Here, we developed a novel three-dimensional (3D) brain cancer chip composed of photo-polymerizable poly(ethylene) glycol diacrylate (PEGDA) hydrogel for drug screening. This chip can be produced after a few seconds of photolithography and requires no silicon wafer, replica molding, and plasma bonding like microfluidic devices made of poly(dimethylsiloxane) (PDMS). We then cultured glioblastoma cells (U87), which formed 3D brain cancer tissues on the chip, and used the GBM chip to perform combinatorial treatment of Pitavastatin and Irinotecan. The results indicate that this chip is capable of high-throughput GBM cancer spheroids formation, multiple-simultaneous drug administration, and a massive parallel testing of drug response. Our approach is easily reproducible, and this chip has the potential to be a powerful platform in cases such as high-throughput drug screening and prolonged drug release. The chip is also commercially promising for other clinical applications, including 3D cell culture and micro-scale tissue engineering.
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A 3D brain tumor chip with PEGDA hydrogel as a carrier can be used as a glioma chip model for drug screening and release tests.
“They injected mepitastatin and irinotecan into the cells, and the results showed that this model can be used as a glioma chip model for drug screening and release tests.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [197]
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- Authors
- Fan, Y., Nguyen, D. T., Akay, Y., Xu, F., & Akay, M.
- Affiliations
- University of Houston (US)
- Xi'an Jiaotong University (CN)
- Corresponding author
- Akay, Metin
- Venue
- Scientific Reports
- Published
- 2016-05-06
- Volume
- 6
- Issue
- 1
- Pages
- 25062-25062
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4858657
- OpenAlex
- W2346064008
- Cited by
- 216
- References
- 32
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 18 · 2023: 22 · 2022: 29 · 2021: 32 · 2020: 23 · 2019: 23 · 2018: 23 · 2017: 14 · 2016: 6
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- glioblastoma, high-throughput drug screening, PEGDA hydrogel, U87 glioblastoma cells, tumor spheroids, pitavastatin, irinotecan, combination therapy, photolithography, microfluidics, 3D cell culture, drug release
- MeSH terms
- Antineoplastic Agents, Brain Neoplasms, Drug Evaluation, Preclinical, Humans, Reproducibility of Results, Spheroids, Cellular, Cell Culture Techniques, Cell Line, Tumor, High-Throughput Screening Assays
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