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Cited by 25Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

Epsin1 modulates synaptic vesicle retrieval capacity at CNS synapses

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Kyung, Jae Won, Bae, Jae Ryul, Kim, Dae-Hwan, Song, Woo Keun, Kim, Sung Hyun·2016·Scientific Reports
DOI 10.1038/srep31997PMC PMC4997357
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Abstract

Synaptic vesicle retrieval is an essential process for continuous maintenance of neural information flow after synaptic transmission. Epsin1, originally identified as an EPS15-interacting protein, is a major component of clathrin-mediated endocytosis. However, the role of Epsin1 in synaptic vesicle endocytosis at CNS synapses remains elusive. Here, we showed significantly altered synaptic vesicle endocytosis in neurons transfected with shRNA targeting Epsin1 during/after neural activity. Endocytosis was effectively restored by introducing shRNA-insensitive Epsin1 into Epsin1-depleted neurons. Domain studies performed on neurons in which domain deletion mutants of Epsin1 were introduced after Epsin1 knockdown revealed that ENTH, CLAP, and NPFs are essential for synaptic vesicle endocytosis, whereas UIMs are not. Strikingly, the efficacy of the rate of synaptic vesicle retrieval (the “endocytic capacity”) was significantly decreased in the absence of Epsin1. Thus, Epsin1 is required for proper synaptic vesicle retrieval and modulates the endocytic capacity of synaptic vesicles.

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  • Epsin1 has been implicated in endocytosis at synapses and can interact with both the plasma membrane and F-actin through its N-terminal ENTH domain and C-terminal tail, respectively.

    “Epsin1 has been implicated in endocytosis at synapses71 and can interact with both the plasma membrane and F-actin through its N-terminal ENTH domain and C-terminal tail, respectively (Supplementary Fig. 7a)72.”

    From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [71]

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Authors
Kyung, J. W., Bae, J. R., Kim, D.-H., Song, W. K., & Kim, S. H.
Affiliations
  • Kyung Hee University (KR)
  • Hanyang University (KR)
  • Gwangju Institute of Science and Technology (KR)
Corresponding author
Kim, Sung Hyun
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-08-25
Volume
6
Issue
1
Pages
31997-31997
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep31997
PMC
PMC4997357
OpenAlex
W2515587612
Cited by
25
References
1
Citations by year
2026: 2 · 2025: 2 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 4 · 2018: 2
FWCI
0.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
epsin1, synaptic vesicle retrieval, synaptic vesicle endocytosis, clathrin-mediated endocytosis, ENTH domain, neuronal activity, shRNA knockdown
MeSH terms
Protein Domains, Animals, Cells, Cultured, Endocytosis, Neurons, Synapses, Synaptic Vesicles, Synaptophysin, Rats, Sprague-Dawley, Adaptor Proteins, Vesicular Transport, RNA Interference, RNA, Small Interfering, Vesicle-Associated Membrane Protein 2, Glucose Transporter Type 1, Rats

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